LOCUS       sequence-233785-       10900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and 
            CD28-CD3z signaling domain. Contains a stuffer fragment flanked by 
            BsmBI sites at the epitope cloning site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Joglekar AV, Leonard MT, Jeppson JD, Swift M, Li G, Wong S, Peng S, 
            Zaretsky JM, Heath JR, Ribas A, Bethune MT, Baltimore D
  TITLE     T cell antigen discovery via signaling and antigen-presenting 
            bifunctional receptors.
  JOURNAL   Nat Methods. 2019 Feb;16(2):191-198. doi: 10.1038/s41592-018-0304-8.
            Epub 2019 Jan 28.
  PUBMED    30700902
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0304-8"; journalName: "Nat Methods"; date: 
            "2019-02"; volume: "16"; issue: "2"; pages: "191-198"
COMMENT     Sequence Label: pCCLc-MND-B2705-SABR-Backbone
FEATURES             Location/Qualifiers
     source          1..10900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        255..634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        635..838
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     789..809
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             853..1033
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1080..1205
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1698..1931
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2116..2160
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2457..2574
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             7038..7103
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             7188..7217
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     LTR             7600..7833
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7905..8026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7942..7961)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7996..8015
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8066..8201
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8221..8240)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8222..8240)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8250..8267)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8250..8266)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8259..8281)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8408..8863
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8495..8514)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8705..8726
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8889..8993
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8994..9062,9063..9854)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9212..9231)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10025..10613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10514..10533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10767..10784
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagcttg
      181 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
      241 attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      301 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      541 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      601 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      661 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      721 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      781 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
      841 tttagtgaac cggggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      901 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      961 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1021 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     1081 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     1141 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     1201 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1261 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1321 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1381 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1441 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1501 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     1561 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1621 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1681 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1741 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1801 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1861 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1921 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1981 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     2041 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     2101 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     2161 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     2221 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     2281 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     2341 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     2401 aacggatctc gacggtatcg ataagctaat tcacaaatgg cagtattcat ccacaatttt
     2461 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2521 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     2581 tattacaggg acagcagaga tccagtttgg gaattagctt gatcgattag tccaatttgt
     2641 taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc accagctgaa
     2701 gcctatagag tacgagccat agatagaata aaagatttta tttagtctcc agaaaaaggg
     2761 gggaatgaaa gaccccacct gtaggtttgg caagctagga tcaaggttag gaacagagag
     2821 acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     2881 ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc
     2941 tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc tcagcagttt
     3001 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
     3061 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
     3121 caataaaaga gcccacaacc cctcactcgg cgcgatctag atctcgaatc gaattcatgg
     3181 cgacgggttc aagaacttcc ctacttcttg catttggcct gctttgtttg ccgtggttac
     3241 aggagggctc ggcaggagac ggttgtaaat gagcacacaa aatacacatg ctaaaatatt
     3301 atattctatg acctttataa aatcaaccaa aatcttcttt ttaataactt tagtatcaat
     3361 aattagaatt tttatgttcc tttttgcaaa cttttaataa aaatgagcaa aataaaaaaa
     3421 cgctagtttt agtaactcgc gttgttttct tcacctttaa taatagctac tccaccactt
     3481 gttcctaagc ggtcagctcc tgcttcaatc attttttgag catcttcaaa tgttctaact
     3541 ccaccagctg ctttaactaa agcattgtct ttaacaactg acttcattag tttaacatct
     3601 tcaaatgttg cacctgattt tgaaaatcct gttgatgttt taacaaattc taatccagct
     3661 tcaacagcta tttcacaagc tttcatgatt tcttcttttg ttaataaaca attttccata
     3721 atacatttaa caacatgtga tccagctgct ttttttacag ctttcatgtc ttctaaaact
     3781 aattcataat ttttgtcttt taatgcacca atatttaata ccatatcaat ttctgttgca
     3841 ccatctttaa ttgcttcaga aacttcgaat gcttttgtag ctgttgtgca tgcacctaga
     3901 ggaaaaccta caacatttgt tattcctaca tttgtgcctt ttaataattc tttacaatag
     3961 cttgttcaat atgaattaac acaaactgtt gcaaaatcaa attcaattgc ttcatcacat
     4021 aattgtttaa tttcagcttt cgtagcatct tgttttaata atgtgtgatc tatatatttg
     4081 tttagtttca ttttttctcc tatatattca tttttaattt taattcttta ataatttcgt
     4141 ctactttaac tttagcgttt tgaacagatt caccaacacc tataaaataa atttttagtt
     4201 taggttcagt tccacttggg cgaacagcaa atcatgactt atcttctaaa taaaatttta
     4261 gtaagtcttg tcctggcata ttatacattc catcgatgta gtcttcaaca ttaacaactt
     4321 taagtccagc aatttgagtt aagggtgttg ctctcaatga tttcattaat ggttcaattt
     4381 ttaatttctt ttcttctggt ttaaaattca agtttaaagt gaaagtgtaa tatgcaccca
     4441 tttctttaaa taaatcttct aaatagtcta ctaatgtttt attttgtttt ttataaaatc
     4501 aagcagcctc tgctattaat atagaagctt gtattccatc tttatctcta gctgagtcat
     4561 caattacata tccataactt tcttcataag caaaaacaaa atttaatccg ttatcttctt
     4621 ctttagcaat ttctctaccc attcatttaa atccagttaa agtttttaca atattaactc
     4681 catatttttc atgagcgatt ctatcaccca aatcacttgt tacaaaactt gaatatagag
     4741 ccggattttt tggaatgcta tttaagcgtt ttagatttga taattttcaa tcaattaaaa
     4801 ttggtcctgt ttgatttcca tctaatctta caaaatgacc atcatgtttt attgccattc
     4861 caaatctgtc agcatctggg tcattcataa taataatatc tgcatcatgt ttaataccat
     4921 attcaagcgg tatttttcat gcaggatcaa attctggatt tggatttaca acatttttaa
     4981 atgtttcatc ttcaaatgca tgctcttcaa cctcaataac gttatatcct gattcacgta
     5041 atatttttgg ggtaaattta gttcctgttc cattaactgc gctaaaaata atttttaaat
     5101 cttttttagc ttcttgctct tttttgtacg tctctggatg cggagggtcc ggcgggggag
     5161 gtagcggtgg tggaggaagc atccagcgta ctccaaagat tcaggtttac tcacgtcatc
     5221 cagcagagaa tggaaagtca aatttcctga attgctatgt gtctgggttt catccatccg
     5281 acattgaagt tgacttactg aagaatggag agagaattga aaaagtggag cattcagact
     5341 tgtctttcag caaggactgg tctttctatc tcttgtacta cactgaattc acccccactg
     5401 aaaaagatga gtatgcctgc cgtgtgaacc atgtgacttt gtcacagccc aagatagtta
     5461 agtgggatcg agacatggga ggtggtggca gcggtggagg gggctccggc ggtgggggat
     5521 ccggcggcgg cggaagcggc tcccactcca tgaggtattt ccacacctcc gtgtcccggc
     5581 ccggccgcgg ggagccccgc ttcatcaccg tgggctacgt ggacgacacg ctgttcgtga
     5641 ggttcgacag cgacgccgcg agtccgagag aggagccgcg ggcgccgtgg atagagcagg
     5701 aggggccgga gtattgggac cgggagacac agatctgcaa ggccaaggca cagactgacc
     5761 gagaggacct gcggaccctg ctccgctgct acaaccagag cgaggccggg tctcacaccc
     5821 tccagaatat gtatggctgc gacgtggggc cggacgggcg cctcctccgc gggtaccacc
     5881 aggacgccta cgacggcaag gattacatcg ccctgaacga ggacctgagc tcctggaccg
     5941 ccgcggacac ggcggctcag atcacccagc gcaagtggga ggcggcccgt gtggcggagc
     6001 agctgagagc ctacctggag ggcgagtgcg tggagtggct ccgcagatac ctggagaacg
     6061 ggaaagaaac actgcagcgc gcggaccccc caaagacaca cgtgacccac caccccatct
     6121 ctgaccatga ggccaccctg aggtgctggg ccctgggctt ctaccctgcg gagatcacac
     6181 tgacctggca gcgggatggc gaggaccaaa ctcaggacac tgagcttgtg gagaccagac
     6241 cagcaggaga tagaaccttc cagaagtggg cagctgtggt ggtgccttct ggagaagagc
     6301 agagatacac atgccatgta cagcatgagg ggctgccgaa gcccctcacc ctgagatggg
     6361 agccgtcttc ccagtccacc gtccccatcg tgggcattgt tgctggcctg gctgtcctag
     6421 cagttgtggt catcggagct gtggtcgctg ctgtgatgtg taggaggaag agctcaggtg
     6481 gaaaaggagg gagctactct caggctgcgt gcagcgacag tgcccagggc tctgatgtgt
     6541 ctctcacagc tcggagcaag aggtctcgcg gtgggcattc cgactacatg aacatgaccc
     6601 ctagaaggcc tggcccaacc agaaagcact accagccata cgcccctccc agagatttcg
     6661 ccgcttatcg aagcgtgaag ttctcccgaa gcgcagatgc cccagcctat cagcagggac
     6721 agaatcagct gtacaacgag ctgaacctgg gaagacggga ggaatacgat gtgctggaca
     6781 aaaggcgggg cagagatcct gagatgggcg gcaaaccaag acggaagaac ccccaggaag
     6841 gtctgtataa tgagctgcag aaagacaaga tggctgaggc ctactcagaa atcgggatga
     6901 agggcgaaag aaggagagga aaaggccacg acggactgta ccaggggctg agtacagcaa
     6961 caaaagacac ctatgacgct ctgcacatgc aggctctgcc accaagaagg gcaaaacgtt
     7021 cgggttcggg tgcgccagta aagcagacat taaactttga tttgctgaaa cttgcaggtg
     7081 atgtagagtc aaatccaggt ccaggggcag gtgccaccgg ccgcgccatg gacgggccgc
     7141 gcctgctgct gttgctgctt ctgggggtgt cccttggagg tgccaaggag cagaagctga
     7201 tcagcgagga ggacctggag atccctggcc gttggattac acggtccaca cccccagagg
     7261 gctcggacag cacagccccc agcacccagg agcctgaggc acctccagaa caagacctca
     7321 tagccagcac ggtggcaggt gtggtgacca cagtgatggg cagctcccag cccgtggtga
     7381 cccgaggcac caccgacaac ctcatccctg tctattgctc catcctggct gctgtggttg
     7441 tgggtcttgt ggcctacata gccttcaaga ggtgagaatt cgttaacctc gagggatccc
     7501 ccggggtcga ctgatcaaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     7561 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7621 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7681 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7741 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7801 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     7861 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     7921 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7981 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     8041 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8101 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8161 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     8221 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     8281 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8341 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     8401 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8461 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8521 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8581 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     8641 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8701 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     8761 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     8821 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     8881 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8941 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9001 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9061 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9121 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9181 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9241 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9301 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9361 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9421 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9481 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9541 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9601 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9661 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9721 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9781 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9841 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9901 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9961 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10021 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10081 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10141 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10201 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10261 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10321 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10381 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10441 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10501 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10561 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10621 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10681 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10741 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    10801 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    10861 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
//
