LOCUS       sequence-233792-        2928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for 
            easy cloning and mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Qi R, Otting G
  TITLE     Mutant T4 DNA polymerase for easy cloning and mutagenesis.
  JOURNAL   PLoS One. 2019 Jan 23;14(1):e0211065. doi: 
            10.1371/journal.pone.0211065. eCollection 2019.
  PUBMED    30673756
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-01-23"; volume: "14(1):e0211065. doi"; pages: " 
            10.1371/journal.pone.0211065. eCollection 2019"
COMMENT     Sequence Label: pETMCSI-T4P_Y320A
FEATURES             Location/Qualifiers
     source          1..2928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             56..78
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(2867..2885)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2881..2928
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaaattaata cgactcacta tagggagacc acaacggttt ccctctagaa ataattttgt
       61 ttaactttaa gaaggagata tacatatgaa agaattttat atcagcatcg aaaccgttgg
      121 caataatatc gttgagcgct acatcgatga aaacggcaaa gaacgtactc gcgaggtgga
      181 gtatctgcca accatgtttc gtcactgcaa agaagagtct aaatataaag acatttatgg
      241 caagaattgt gccccacaga aatttccgtc tatgaaggat gcgcgtgact ggatgaagcg
      301 gatggaggat atcgggctgg aggcgttagg catgaacgat tttaaattag cctacatttc
      361 ggatacctat ggctccgaga tcgtatatga tcgtaaattt gttcgcgtag cgaactgtga
      421 catcgaggtg acgggggata aattcccgga cccaatgaaa gcagaatatg aaatcgacgc
      481 tatcactcac tatgacagta tcgatgaccg cttctatgtc ttcgatttac tgaatagcat
      541 gtacggttca gtttccaaat gggatgcaaa actggctgcg aagcttgatt gcgaaggagg
      601 tgatgaagtt ccgcaggaaa ttctggatcg ggtcatttat atgccattcg ataacgaacg
      661 tgacatgctc atggagtata tcaacctctg ggagcaaaaa cgccccgcga tctttacagg
      721 ttggaacatc gagggtttcg atgtgccgta cattatgaac cgtgtcaaaa tgatccttgg
      781 ggaacgttct atgaaacgtt tcagtccgat cgggcgcgtg aaatccaagc ttattcaaaa
      841 tatgtatgga agcaaagaaa tttacagcat tgatggggtt tcaatcctgg attatttgga
      901 cctttataaa aaattcgctt ttactaacct gccgtcattt tcgctggaaa gtgtagcaca
      961 gcacgaaaca aaaaaaggta agcttccgta tgatgggcca atcaataaac ttcgtgaaac
     1021 gaaccatcag cgctatattt ccgcaaacat tatcgacgta gaaagcgtgc aggcaatcga
     1081 taaaattcgc ggttttattg atctggtact gagcatgtcg tattatgcaa aaatgccatt
     1141 tagtggtgta atgagcccga tcaaaacctg ggatgcgatt atcttcaata gcctgaaagg
     1201 tgagcataaa gttattccgc agcagggctc tcacgtgaaa caaagctttc cgggcgcgtt
     1261 tgtgtttgaa ccgaaaccaa tcgctcgtcg ctacattatg agctttgact taacatctct
     1321 ctacccgtcc attattcggc aagtcaatat cagtccggaa acgattcggg gccaatttaa
     1381 agttcacccg atccatgagt atattgcagg caccgcgcca aaaccgtccg acgaatacag
     1441 ctgctcgcca aacggttgga tgtatgacaa acatcaggaa ggaattatcc caaaagagat
     1501 cgctaaagtc ttctttcaac gcaaagattg gaaaaaaaaa atgtttgcgg aggaaatgaa
     1561 cgcggaagcg attaagaaaa ttatcatgaa aggtgctggg agctgtagca cgaaaccgga
     1621 ggtggagcgt tatgtgaaat ttagcgacga ttttctgaat gaactcagca attatacgga
     1681 gtctgttctg aactctctga ttgaagaatg tgaaaaggcg gcgaccctgg cgaatacaaa
     1741 tcagttaaat cggaaaattc ttattaatag tctgtatggt gcgctcggga acatccattt
     1801 tcgttattac gacctgcgga atgccaccgc aattaccatt tttggccagg tcggaatcca
     1861 gtggattgcc cgtaaaatta atgagtatct caacaaggtc tgtggcacca acgacgaaga
     1921 tttcatcgct gcgggtgata cagatagcgt ttatgtctgt gtcgacaagg ttattgaaaa
     1981 agttgggctg gatcggttca aagaacagaa cgatctcgta gaatttatga atcagttcgg
     2041 caaaaaaaag atggagccta tgattgacgt cgcttaccgc gagctgtgtg attacatgaa
     2101 caatcgcgaa caccttatgc acatggatcg cgaggcaatt tcatgtccgc ctctgggctc
     2161 gaaaggtgta gggggtttct ggaaagcaaa aaaacgttat gcgctcaatg tttacgatat
     2221 ggaagataaa cgcttcgcgg agccgcacct caaaatcatg ggaatggaaa cacagcagag
     2281 ctctaccccg aaagctgttc aagaagccct ggaggaaagt attcgtcgta ttctgcaaga
     2341 aggtgaggag agcgtgcaag aatattacaa gaactttgaa aaggagtatc gtcagctgga
     2401 ttacaaagta attgcggagg ttaaaactgc aaatgacatt gcaaaatatg atgataaggg
     2461 ttggcccggg tttaaatgtc ctttccacat tcgtggcgtc ctgacgtatc gtcgcgccgt
     2521 cagcgggctg ggtgtcgcgc cgatcttgga cggcaacaaa gttatggtcc tccctctgcg
     2581 tgaagggaac ccttttggtg ataaatgtat tgcgtggcct tcgggcaccg aattaccgaa
     2641 agaaatccgt tccgatgtgc tttcgtggat cgatcatagc actttgttcc agaaatcttt
     2701 tgttaaacct ctggcgggca tgtgcgaaag cgcgggtatg gattacgaag aaaaagcgtc
     2761 actggacttt ctgtttggtt gaattcgagc tcccgggtac catggcatgc atcgatagat
     2821 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     2881 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttg
//
