LOCUS       sequence-233794-        3843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes full-length engineered CD64 with C-terminal Twin-Strep tag 
            to be expressed in a baculovirus/insect cell expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Le LTM, Nyengaard JR, Golas MM, Sander B
  TITLE     Vectors for Expression of Signal Peptide-Dependent Proteins in 
            Baculovirus/Insect Cell Systems and Their Application to Expression 
            and Purification of the High-Affinity Immunoglobulin Gamma Fc 
            Receptor I in Complex with Its Gamma Chain.
  JOURNAL   Mol Biotechnol. 2018 Jan;60(1):31-40. doi: 
            10.1007/s12033-017-0041-8.
  PUBMED    29143175
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biotechnol"; date: "2018-01"; volume: "60"; issue: "1"; pages: 
            "31-40"
COMMENT     Sequence Label: pGS-CD64-2
FEATURES             Location/Qualifiers
     source          1..3843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1925..1930,1931..1947,1948..1953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2134..2225
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2150..2167
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(3201..3206,3207..3230,3231..3266,3267..3290)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     polyA_signal    3423..3557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3460..3479)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3514..3533
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3678..3843
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg taactataac ggtcctaagg tagcgagttt aaacactagt
     2101 atcgattcgc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     2161 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     2221 taaatattcc ggattattca taccgtccca ccatcgggcg cggatcccgg tccgaagcgc
     2281 gcggaattca tgggtgcagt gcttcgcagc ttgctcgcct gttccttctg cgtcctgctg
     2341 cgtgctgcga attcggtacc gctagccaag tggatactcc aaaggccgtg ataaccttgc
     2401 aaccaccttg ggtgtccgtc tttcaggaag agtcggttac actgcactgt gaggtcccac
     2461 acttgcccgg tagttccagc actcagtggt tccttaacgg tacggccatc cagacgtcaa
     2521 cccctactta ccgcatcacc agtgcttctg ttgacgactc gggtgaatac aggtgccaac
     2581 gtggtttgtc cggccgcagt gatccaatcc aactggaggt tcaccgtggt tggctgctgc
     2641 tgcaggtgag tagccgtgtg ttgacggagg gagaacccct ggccctgcgc tgccatgctt
     2701 ggaaggataa acttgtatac aacgtattgt actatcgtaa cggcaaggca ttcaagttct
     2761 tccactggaa cagcaacctc acgatcctga aaacaaatat gtctcacagc ggcacatatc
     2821 attgtagcgg catgggtaag caccgctaca cgtcagctgg catttccgta acagtgaagg
     2881 agctgttccc agcgccagtc cttacggcat cggtaacctc gccgcttctc gaaggcaatc
     2941 ccgttacact cagctgcgag accaaactgc tcctccaacg tcctggtctg cagttgtact
     3001 tctcattcta catgggttca aagactctca ggggaaggga cacatcgagc gagtatcaga
     3061 tacttaccgc cagacgcgag gactccggcc tgtactggtg tgaagctgcc accgaggatg
     3121 gcaacgttct taagcgctct cccgaattgg agttgcaagt gctcggcctg cagctgccaa
     3181 cgcctgtagt cgactctaga agtgcttgga gtcatccaca attcgaaaaa ggtggaggtt
     3241 ccggaggtgg atcgggaggt tctgcctggt ctcatccaca atttgagaag taaaagcttg
     3301 tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     3361 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     3421 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3481 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3541 cttatcatgt ctggatctga tcactgcttg agcctagaag atccggctgc taacaaagcc
     3601 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactatcata acccctaggg
     3661 tatacccatc taattggaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
     3721 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
     3781 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
     3841 aca
//
