LOCUS       sequence-233796-        2463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human full-length FCER1G to be expressed in a 
            baculovirus/insect cell expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Le LTM, Nyengaard JR, Golas MM, Sander B
  TITLE     Vectors for Expression of Signal Peptide-Dependent Proteins in 
            Baculovirus/Insect Cell Systems and Their Application to Expression 
            and Purification of the High-Affinity Immunoglobulin Gamma Fc 
            Receptor I in Complex with Its Gamma Chain.
  JOURNAL   Mol Biotechnol. 2018 Jan;60(1):31-40. doi: 
            10.1007/s12033-017-0041-8.
  PUBMED    29143175
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biotechnol"; date: "2018-01"; volume: "60"; issue: "1"; pages: 
            "31-40"
COMMENT     Sequence Label: pGS-FCER1G-1
FEATURES             Location/Qualifiers
     source          1..2463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..125
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     primer_bind     complement(447..466)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    491..539
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    741..774
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      776..1132
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             1597..2388
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgatactagt atacggacct ttaattcaac ccaacacaat atattatagt taaataagaa
       61 ttattatcaa atcatttgta tattaattaa aatactatac tgtaaattac attttattta
      121 caatcactcg acgaagactt gatcacccgg gatctcgaga tgatccccgc tgtggttctt
      181 ctgcttctcc tcttggttga gcaggctgct gctctcggag aaccacaact ctgttacatc
      241 ctggacgcga tcttgtttct gtacggtatc gtgctcaccc tgctgtactg tcgtcttaaa
      301 attcaagttc gcaaggcagc cattaccagt tatgagaaaa gcgacggtgt ctataccggc
      361 ctctccacgc gtaaccagga gacttacgag actctgaaac atgaaaagcc cccccaataa
      421 ggtaccggga gatgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
      481 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
      541 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
      601 ggaccaatac gcccgcgttt cttccttttc cccaccccaa cccccaagtt cgggtgaagg
      661 cccagggctc gcagccaacg tcggggcggc aagccctgcc atagccacta cgggtacgtt
      721 taaacccatg tgcctggcag ataacttcgt ataatgtatg ctatacgaag ttatggtacg
      781 tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt
      841 ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc
      901 tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact
      961 taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa
     1021 gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct
     1081 tagagcctct caaagcaatt ttgagtgaca caggaacact taacggctga catgggaatt
     1141 agcttcacgc tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta
     1201 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctggg aggcagaata
     1261 aatgatcata tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc
     1321 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa gtagcgtatg
     1381 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     1441 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     1501 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     1561 cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa
     1621 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     1681 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     1741 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     1801 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     1861 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     1921 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     1981 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     2041 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     2101 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     2161 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     2221 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     2281 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     2341 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt
     2401 cgttcaagcc gacggatcta tgtcgggtgc ggagaaagag gtaatgaaat ggcacctagg
     2461 tat
//
