LOCUS       sequence-233798-        5124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Intermediate plasmid 1 to build tobacco etch virus recombinant 
            clones.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Daros JA
  TITLE     Use of Potyvirus Vectors to Produce Carotenoids in Plants.
  JOURNAL   Methods Mol Biol. 2020;2083:303-312. doi: 
            10.1007/978-1-4939-9952-1_23.
  PUBMED    31745931
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2020"; volume: "2083:303-312. doi"; pages: " 
            10.1007/978-1-4939-9952-1_23"
COMMENT     Sequence Label: pTV1
FEATURES             Location/Qualifiers
     source          1..5124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     256..276
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        265..311
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(3673..3690)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3807..4352)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3887..3906)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4370..5029)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4944..4963
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcggccgcga ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga
       61 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc
      121 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
      181 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
      241 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
      301 tttggagagg aaaataacaa atctcaacac aacatataca aaacaaacga atctcaagca
      361 atcaagcatt ctacttctat tgcagcaatt taaatcattt cttttaaagc aaaagcaatt
      421 ttctgaaaat tttcaccatt tacgaacgat agccatggca ctcatctttg gcacagtcaa
      481 cgctaacatc ctgaaggaag tgttcggtgg agctcgtatg gcttgcgtta ccagcgcaca
      541 tatggctgga gcgaatggaa gcattttgaa gaaggcagaa gaaacctctc gtgcaatcat
      601 gcacaaacca gtgatcttcg gagaagacta cattaccgag gcagacttgc cttacacacc
      661 actccattta gaggtcgatg ctgaaatgga gcggatgtat tatcttggtc gtcgcgcgct
      721 cacccatggc aagagacgca aagtttctgt gaataacaag aggaacagga gaaggaaagt
      781 ggccaaaacg tacgtggggc gtgattccat tgttgagaag attgtagtgc cccacaccga
      841 gagaaaggtt gataccacag cagcagtgga agacatttgc aatgaagcta ccactcaact
      901 tgtgcataat agtatgccaa agcgtaagaa gcagaaaaac ttcttgcccg ccacttcact
      961 aagtaacgtg tatgcccaaa cttggagcat agtgcgcaaa cgccatatgc aggtggagat
     1021 cattagcaag aagagcgtcc gagcgagggt caagagattt gagggctcgg tgcaattgtt
     1081 cgcaagtgtg cgtcacatgt atggcgagag gaaaagggtg gacttacgta ttgacaactg
     1141 gcagcaagag acacttctag accttgctaa aagatttaag aatgagagag tggatcaatc
     1201 gaagctcact tttggttcaa gtggcctagt tttgaggcaa ggctcgtacg gacctgcgca
     1261 ttggtatcga catggtatgt tcattgtacg cggtcggtcg gatgggatgt tggtggatgc
     1321 tcgtgcgaag gtaacgttcg ctgtttgtca ctcaatgaca cattatagcg acaaatcaat
     1381 ctctgaggca ttcttcatac catactctaa gaaattcttg gagttgaggc cagatggaat
     1441 ctcccatgag tgtacaagag gagtatcagt tgagcggtgc ggtgaggtgg ctgcaatcct
     1501 gacacaagca ctttcaccgt gtggtaagat cacatgcaaa cgttgcatgg ttgaaacacc
     1561 tgacattgtt gagggtgagt cgggagacag tgtcaccaac caaggtaagc tcctagcaat
     1621 gctgaaagaa cagtatccag atttcccaat ggccgagaaa ctactcacaa ggtttttgca
     1681 acagaaatca ctagtaaata caaatttgac agcctgcgtg agcgtcaaac aactcattgg
     1741 tgaccgcaaa caagctccat tcacacacgt actggctgtc agcgaaattc tgtttaaagg
     1801 caataaacta acaggggccg atctcgaaga ggcaagcaca catatgcttg aaatagcaag
     1861 gttcttgaac aatcgcactg aaaatatgcg cattggccac cttggttctt tcagaaataa
     1921 aatctcatcg aaggcccatg tgaataacgc actcatgtgt gataatcaac ttgatcagaa
     1981 tgggaatttt atttggggac taaggggtgc acacgcaaag aggtttctta aaggattttt
     2041 cactgagatt gacccaaatg aaggatacga taagtatgtt atcaggaaac atatcagggg
     2101 tagcagaaag ctagcaattg gcaatttgat aatgtcaact gacttccaga cgctcaggca
     2161 acaaattcaa ggcgaaacta ttgagcgtaa agaaattggg aatcactgca tttcaatgcg
     2221 gaatggtaat tacgtgtacc catgttgttg tgttactctt gaagatggta aggctcaata
     2281 ttcggatcta aagcatccaa cgaagagaca tctggtcatt ggcaactctg gcgattcaaa
     2341 gtacctagac cttccagttc tcaatgaaga gaaaatgtat atagctaatg aaggttattg
     2401 ctacatgaac attttctttg ctctactagt gaatgtcaag gaagaggatg caaaggactt
     2461 caccaagttt ataagggaca caattgttcc aaagcttgga gcgtggccaa caatgcaaga
     2521 tgttgcaact gcatgctact tactttccat tctttaccca gatgtcctga gtgctgaatt
     2581 acccagaatt ttggttgatc atgacaacaa aacaatgcat gttttggatt cgtatgggtc
     2641 tagaacgaca ggataccaca tgttgaaaat gaacacaaca tcccagctaa ttgaattcgt
     2701 tcattcaggt ttggaatccg aaatgaaaac ttacaatgtt ggagggatga accgagatat
     2761 ggtcacacaa ggtgcaattg agatgttgat caagtccata tacaaaccac atctcatgaa
     2821 gcagttactt gaggaggagc catacataat tgtcctggca atagtctccc cttcaatttt
     2881 aattgccatg tacaactctg gaacttttga gcaggcgtta caaatgtggt tgccaaatac
     2941 aatgaggtta gctaacctcg ctgccatctt gtcagccttg gcgcaaaagt taactttggc
     3001 agacttgttc gtccagcagc gtaatttgat taatgagtat gcgcaggtaa ttttggacaa
     3061 tctgattgac ggtgtcaggg ttaaccattc gctatcccta gcaatggaaa ttgttactat
     3121 taagctggcc acccaagaga tggacatggc gttgagggaa ggtggctatg ctgtgacctc
     3181 tgaaaaggtg catgaaatgt tggaaaaaaa ctatgtaaag gctttgaagg atgcatggga
     3241 cgaattaact tggttggaaa aattctccgc aatcaggcat tcaagaaagc tcttgaaatt
     3301 tgggcgaaag cctttaatca tgaaaaacac cgtagattgc ggcggacata tagacttgtc
     3361 tgtgaaatcg cttttcaagt tccacttgga actcctgaag ggaaccatct caagagccgt
     3421 aaatggtggt gcaagaaagg taagagtagc gaagaatgcc atgacaaaag gggtttttct
     3481 caaaatctac agcatgcttc ctgacgtcgg cgcctaaggg gcgagatctg gcggggcccg
     3541 cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc
     3601 ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat
     3661 atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga
     3721 aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga
     3781 agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac
     3841 gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg
     3901 tttccccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
     3961 attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc
     4021 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt
     4081 atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc
     4141 agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa
     4201 ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt
     4261 tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc
     4321 aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat tacgccccgc
     4381 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     4441 cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     4501 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     4561 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     4621 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     4681 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     4741 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     4801 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     4861 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     4921 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     4981 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     5041 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     5101 ggtgaaagtt ggaacccggg ggcg
//
