LOCUS       sequence-233799-        4847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Intermediate plasmid 3 to build tobacco etch virus recombinant 
            clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Daros JA
  TITLE     Use of Potyvirus Vectors to Produce Carotenoids in Plants.
  JOURNAL   Methods Mol Biol. 2020;2083:303-312. doi: 
            10.1007/978-1-4939-9952-1_23.
  PUBMED    31745931
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2020"; volume: "2083:303-312. doi"; pages: " 
            10.1007/978-1-4939-9952-1_23"
COMMENT     Sequence Label: pTV3
FEATURES             Location/Qualifiers
     source          1..4847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1984..2004
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(3374..3391)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3508..4053)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3588..3607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4071..4730)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4645..4664
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cctaaggatt tcccaccatt tcctcaaaag ctgaaattta gagagccaca aagggaagag
       61 cgcatatgtc ttgtgacaac caacttccaa actaagagca tgtctagcat ggtgtcagac
      121 actagttgca cattcccttc atctgatggc atattctgga agcattggat tcaaaccaag
      181 gatgggcagt gtggcagtcc attagtatca actagagatg ggttcattgt tggtatacac
      241 tcagcatcga atttcaccaa cacaaacaat tatttcacaa gcgtgccgaa aaacttcatg
      301 gaattgttga caaatcagga ggcgcagcag tgggttagtg gttggcgatt aaatgctgac
      361 tcagtattgt gggggggcca taaagttttc atgagcaaac ctgaagagcc ttttcagcca
      421 gttaaggaag cgactcaact catgagtgaa ttggtgtact cgcaagggga gaagaggaaa
      481 tgggtcgtgg aagcactgtc agggaacttg aggccagtgg ctgagtgtcc cagtcagtta
      541 gtcacaaagc atgtggttaa aggaaagtgt cccctctttg agctctactt gcagttgaat
      601 ccagaaaagg aagcatattt taaaccgatg atgggagcat ataagccaag tcgacttaat
      661 agagaggcgt tcctcaagga cattctaaaa tatgctagtg aaattgagat tgggaatgtg
      721 gattgtgact tgctggagct tgcaataagc atgctcatca caaagctcaa ggcgttagga
      781 ttcccaactg tgaactacat cactgaccca gaggaaattt ttagtgcatt gaatatgaaa
      841 gcagctatgg gagcactata caaaggcaag aagaaagaag ctctcagcga gctcacacta
      901 gatgagcagg aggcaatgct caaagcaagt tgcctgcgac tgtatacggg aaagctggga
      961 atttggaatg gctcattgaa agcagagttg cgtccaattg agaaggttga aaacaacaaa
     1021 acgcgaactt tcacagcagc accaatagac actcttcttg ctggtaaagt ttgcgtggat
     1081 gatttcaaca atcaatttta tgatctcaac ataaaggcac catggacagt tggtatgact
     1141 aagttttatc aggggtggaa tgaattgatg gaggctttac caagtgggtg ggtgtattgt
     1201 gacgctgatg gttcgcaatt cgacagttcc ttgactccat tcctcattaa tgctgtattg
     1261 aaagtgcgac ttgccttcat ggaggaatgg gatattggtg agcaaatgct gcgaaatttg
     1321 tacactgaga tagtgtatac accaatcctc acaccggatg gtactatcat taagaagcat
     1381 aaaggcaaca atagcgggca accttcaaca gtggtggaca acacactcat ggtcattatt
     1441 gcaatgttat acacatgtga gaagtgtgga atcaacaagg aagagattgt gtattacgtc
     1501 aatggcgatg acctattgat tgccattcac ccagataaag ctgagaggtt gagtggattc
     1561 aaagaatctt tcggagagtt gggcctgaaa tatgaatttg actgcaccac cagggacaag
     1621 acacagttgt ggttcatgtc acacagggct ttggagaggg atggcatgta tataccaaag
     1681 ctagaagaag aaaggattgt ttctattttg gaatgggaca gatccaaaga gccgtcacat
     1741 aggcttgaag ccatctgtgc atcaatgatc gaagcatggg gttatgacaa gctggttgaa
     1801 gaaatccgca atttctatgc atgggttttg gaacaagcgc cgtattcaca gcttgcagaa
     1861 gaaggaaagg cgccatatct ggctgagact gcgcttaagt ttttgtacac atctcagcac
     1921 ggaacaaact ctgagataga agagtattta aaagtgttgt atgattacga tattccaacg
     1981 actgagaatc tttattttca gagtggcact gtgggtgctg gtgttgacgc tggtaagaag
     2041 aaagatcaaa aggatgataa agtcgctgag caggcttcaa aggataggga tgttaatgct
     2101 ggaacttcag gaacattctc agttccacga ataaatgcta tggccacaaa acttcaatat
     2161 ccaaggatga ggggagaggt ggttgtaaac ttgaatcacc ttttaggata caagccacag
     2221 caaattgatt tgtcaaatgc tcgagccaca catgagcagt ttgccgcgtg gcatcaggca
     2281 gtgatgacag cctatggagt gaatgaagag caaatgaaaa tattgctaaa tggatttatg
     2341 gtgtggtgca tagaaaatgg gacttcccca aatttgaacg gaacttgggt tatgatggat
     2401 ggtgaggagc aagtttcata cccgctgaaa ccaatggttg aaaacgcgca gccaacactg
     2461 aggcaaatta tgacacactt cagtgacctg gctgaagcgt atattgagat gaggaatagg
     2521 gagcgaccat acatgcctag gtatggtcta cagagaaaca ttacagacat gagtttgtca
     2581 cgctatgcgt tcgacttcta tgagctaact tcaaaaacac ctgttagagc gagggaggcg
     2641 catatgcaaa tgaaagctgc tgcagtacga aacagtggaa ctaggttatt tggtcttgat
     2701 ggcaacgtgg gtactgcaga ggaagacact gaacggcaca cagcgcacga tgtgaaccgt
     2761 aacatgcaca cactattagg ggtccgccag tgatagtttc tgcgtgtctt tgctttccgc
     2821 ttttaagctt attgtaatat atatgaatag ctattcacag tgggacttgg tcttgtgttg
     2881 aatggtatct tatatgtttt aatatgtctt attagtctca ttacttaggc gaacgacaaa
     2941 gtgaggtcac ctcggtctaa ttctcctatg tagtgcgaga aaaaaaaaaa aaaaaaaaaa
     3001 aaaaaaaaaa aaaaaaaaaa aaaagatctc gctgaaatca ccagtctctc tctacaaatc
     3061 tatctctctc tattttctcc ataaataatg tgtgagtagt ttcccgataa gggaaattag
     3121 ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     3181 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccaggggccc
     3241 gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg
     3301 cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga
     3361 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
     3421 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg
     3481 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca
     3541 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
     3601 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     3661 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     3721 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     3781 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
     3841 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
     3901 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
     3961 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
     4021 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa ttacgccccg
     4081 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     4141 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     4201 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     4261 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     4321 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     4381 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     4441 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     4501 attgccatac ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     4561 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     4621 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     4681 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     4741 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     4801 tggtgaaagt tggaacccgg gggcggcggc cgcggcggac gtcggcg
//
