LOCUS       sequence-233803-        2390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid donor for expression of proteins under the control of a 
            minimal HSP70 promoter in baculovirus/insect cell systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Golas MM, Jayaprakash S, Le LTM, Zhao Z, Heras Huertas V, Jensen IS,
            Yuan J, Sander B
  TITLE     Modulating the Expression Strength of the Baculovirus/Insect Cell 
            Expression System: A Toolbox Applied to the Human Tumor Suppressor 
            SMARCB1/SNF5.
  JOURNAL   Mol Biotechnol. 2018 Nov;60(11):820-832. doi: 
            10.1007/s12033-018-0107-2.
  PUBMED    30178299
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biotechnol"; date: "2018-11"; volume: "60"; issue: "11"; pages:
            "820-832"
COMMENT     Sequence Label: pGS-BacD-23003
FEATURES             Location/Qualifiers
     source          1..2390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     197..218
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(322..341)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    366..414
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    616..649
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      651..1007
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             1371..2165
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQLTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1425..1444)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2035..2054
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gatactagtc gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca
       61 aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa
      121 caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca
      181 accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg aatacaagaa
      241 gacttgatca cccgggatct cgagccatgg tgctagcagc tgatgcatag catgcggtac
      301 cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
      361 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
      421 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattgggacc
      481 aatacgcccg cgtttcttcc ttttccccac cccaaccccc aagttcgggt gaaggcccag
      541 ggctcgcagc caacgtcggg gcggcaagcc ctgccatagc cactacgggt acgtttaaac
      601 ccatgtgcct ggcagataac ttcgtataat gtatgctata cgaagttatg gtacgtacta
      661 agctctcatg tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac
      721 gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg
      781 tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat
      841 agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt
      901 taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag
      961 cctctcaaag caattttgag tgacacagga acacttaacg gctgacatgg gaattagctt
     1021 cacgctgccg caagcactca gggcgcaagg gctgctaaag gaagcggaac acgtagaaag
     1081 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
     1141 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
     1201 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
     1261 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
     1321 ggcgcagggg atcaagatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     1381 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     1441 gggcacaact gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     1501 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     1561 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     1621 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     1681 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     1741 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     1801 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     1861 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     1921 tcgtcgtgac acatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     1981 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     2041 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     2101 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     2161 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     2221 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     2281 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccgg
     2341 gatctatgtc gggtgcggag aaagaggtaa tgaaatggca cctaggtatc
//
