LOCUS       sequence-233810-        3267 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gli activity reporter, mouse transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Balaskas N, Ribeiro A, Panovska J, Dessaud E, Sasai N, Page KM, 
            Briscoe J, Ribes V
  TITLE     Gene regulatory logic for reading the Sonic Hedgehog signaling 
            gradient in the vertebrate neural tube.
  JOURNAL   Cell. 2012 Jan 20;148(1-2):273-84. doi: 10.1016/j.cell.2011.10.047.
  PUBMED    22265416
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-01-20"; volume: "148(1-2):273-84. doi"; pages: " 
            10.1016/j.cell.2011.10.047"
COMMENT     Sequence Label: GBSGFP insulator
FEATURES             Location/Qualifiers
     source          1..3267
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..29
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     14..30
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(1422..1424,1425..1427,1428..2141)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1422..1424,1425..1427,1428..2138)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1467..1488)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1728..1747)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2075..2096
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2274..2408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2311..2330)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2365..2384
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cgagcggccg cttcgaggtc gacggtatcg ataagcttga ttcgaggatc cttatgacgg
       61 aggctaacaa gcagggaaca cccaagtaga agctggctgt cagatcctta tgacggaggc
      121 taacaagcag ggaacaccca agtagaagct ggctgtcaga tccttatgac ggaggctaac
      181 aagcagggaa cacccaagta gaagctggct gtcagatcct tatgacggag gctaacaagc
      241 agggaacacc caagtagaag ctggctgtca gatccttatg acggaggcta acaagcaggg
      301 aacacccaag tagaagctgg ctgtcagatc cttatgacgg aggctaacaa gcagggaaca
      361 cccaagtaga agctggctgt cagatcctta tgacggaggc taacaagcag ggaacaccca
      421 agtagaagct ggctgtcaga tccttatgac ggaggctaac aagcagggaa cacccaagta
      481 gaagctggct gtcagatctg tcgaatcgaa ttcctgcagc ccggcgcgcc cgggcgatcg
      541 cgggtaccga gctccaggaa catccaaact gagcagccgg ggtccccccc accccccacc
      601 ccgcccctcc cggcaacttt gagcctgtgc tgggacagag cctctagttc ctaaattagt
      661 ccatgaggtc agaggcagca ctgccattgt aacgcgattg gagaggatca cgtcaccgga
      721 cacgccccca ggcatctccc tgggtctcct aaacttggcg gggagaagtt ttagccctta
      781 agttttagcc tttaaccccc atattcagaa ctgtgcgagt tggcgaaacc ccacaaatca
      841 caacaaactg tacacaacac cgagctagag gtgatctttc ttgtccattc cacacaggcc
      901 ttagtaatgc gtcgccatag caacagtgtc actagtagca ccagcacttc cccacaccct
      961 ccccctcagg aatccgtact ctccagtgaa ccccagaaac ctctggagag ttctggacaa
     1021 gggcggaacc cacaactccg attactcaag ggaggcgggg aagctccacc agacgcgaaa
     1081 ctgctggaag attcctggcc ccaaggcctc ctccggctcg ctgattggcc cagcggagag
     1141 tgggcggggc cggtgaagac tccttaaagg cgcagggcgg cgagcaggtc accagacgct
     1201 gacagctact cagaaccaaa tctggttcca tccagagaca agcgaagaca agagaagcag
     1261 agcgagcggc gcgttcccga tcctcggcca ggaccagcct tccccagagc atccctgccg
     1321 cggagcgcaa ccttcccagg agcatccctg ccgcggagcg caactttccc cggagcatcc
     1381 acgccgcgga gcgcagcctt ccagaagcag agcgcggcgc catggtgagc aagggcgagg
     1441 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1501 agttcagcgt gtccggcgag ggcgagggcg atgccacnta cggcaagctg accctgaagt
     1561 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1621 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1681 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1741 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1801 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1861 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1921 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1981 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     2041 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2101 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagat
     2161 aagtaatgat cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc
     2221 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2281 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2341 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2401 tctggatccc cgggtaccga gctcgctaga gggacagccc ccccccaaag cccccaggga
     2461 tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg
     2521 tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga
     2581 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac
     2641 ctggggggat acggggaaaa agctttaggc tgaaagagag atttagaatg acagaatcat
     2701 agaacggcct gggttgcaaa ggagcacagt gctcatccag atccaacccc ctgctatgtg
     2761 cagggtcatc aaccagcagc ccaggctgcc cagagccaca tccagcctgg ccttgaatgc
     2821 ctgcagggat ggggcatcca cagcctcctt gggcaacctg ttcagtgcgt caccaccctc
     2881 tgggggaaaa actgcctcct catatccaac ccaaacctcc cctgtctcag tgtaaagcca
     2941 ttcccccttg tcctatcaag ggggagtttg ctgtgacatt gttggtctgg ggtgacacat
     3001 gtttgccaat tcagtgcatc acggagaggc agatcttggg gataaggaag tgcaggacag
     3061 catggacgtg ggacatgcag gtgttgaggg ctctgggaca ctctccaagt cacagcgttc
     3121 agaacagcct taaggataag aagataggat agaaaggaca aagagcaagt taaaacccag
     3181 catggagagg agcacaaaaa ggccacagac actgctggtc cctgtgtctg agcctgcatg
     3241 tttgatggtg tctggatgca agcagaa
//
