LOCUS       sequence-233815-        4785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human full-length LSD1 to be expressed in a 
            baculovirus/insect cell expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Inui K, Zhao Z, Yuan J, Jayaprakash S, Le LTM, Drakulic S, Sander B,
            Golas MM
  TITLE     Stepwise assembly of functional C-terminal REST/NRSF transcriptional
            repressor complexes as a drug target.
  JOURNAL   Protein Sci. 2017 May;26(5):997-1011. doi: 10.1002/pro.3142. Epub 
            2017 Mar 12.
  PUBMED    28218430
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3142"; 
            journalName: "Protein Sci"; date: "2017-05"; volume: "26"; issue: 
            "5"; pages: "997-1011"
COMMENT     Sequence Label: pIDS-LSD1fl
FEATURES             Location/Qualifiers
     source          1..4785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..125
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     primer_bind     complement(2769..2788)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2813..2861
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    3063..3096
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      3098..3454
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             3919..4710
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgatactagt atacggacct ttaattcaac ccaacacaat atattatagt taaataagaa
       61 ttattatcaa atcatttgta tattaattaa aatactatac tgtaaattac attttattta
      121 caatcactcg acgaagactt gatcacccgg gatctcgaga tgttgtccgg aaagaaggcc
      181 gccgctgccg ccgccgccgc tgccgctgct gccacaggta ccgaagctgg tcctggaacc
      241 gccggaggtt ctgaaaacgg atccgaagtc gctgcccaac ccgctggtct gtcaggtccc
      301 gccgaagttg gacctggagc tgtgggcgaa cgtactccaa ggaaaaagga accccctcgt
      361 gcctcccctc ccggtggact ggctgagcca cctggatccg ccggacctca ggctggtccc
      421 acagtcgtgc ccggttccgc tacacccatg gagacaggta tcgctgaaac ccctgaaggt
      481 cgtcgtacct cacgtcgtaa gcgtgccaag gtggaataca gagaaatgga tgagagtctg
      541 gctaacttgt cagaggatga gtactactct gaagaggaac gcaacgctaa ggccgagaag
      601 gagaaaaagt tgcctccccc tccaccacag gctcctccag aggaggaaaa cgaatcagaa
      661 cccgaagagc caagcggtgt cgagggcgct gctttccaaa gccgtttgcc tcatgatcgt
      721 atgacatccc aggaggccgc ctgcttccct gacatcatca gcggccccca acagacacag
      781 aaagttttcc tcttcatcag gaacagaacc ctccaactgt ggctcgacaa cccaaagatt
      841 cagctcactt tcgaggctac actgcaacaa ctcgaagctc cttataactc tgatactgtg
      901 ctggtccaca gagtccactc ttacctcgaa cgccatggtc tgatcaactt cggaatctac
      961 aagcgcatta agcccctgcc taccaaaaag actggtaaag tgatcatcat cggttcaggc
     1021 gttagcggcc tggctgctgc ccgccagctc caaagtttcg gtatggacgt caccctgctg
     1081 gaggctaggg accgcgtcgg aggcagggtt gctacattca ggaagggcaa ctacgttgcc
     1141 gacctcggcg ccatggttgt taccggcctg ggtggtaacc ccatggctgt ggtgagcaag
     1201 caggtcaaca tggaactcgc caagattaag caaaagtgcc ctctctacga agctaatggc
     1261 caggccgtgc caaaggaaaa ggacgaaatg gtggaacagg agttcaaccg cctgctggag
     1321 gccactagct acctctccca ccagctggac ttcaacgtgc tgaataacaa acctgttagt
     1381 ctgggtcaag ccctggaagt ggttatccag ctgcaagaga agcacgttaa ggacgagcag
     1441 atcgaacact ggaagaagat cgttaagact caagaagagc tgaaagaact cctcaacaag
     1501 atggttaacc tgaaggaaaa aatcaaggag ctgcaccaac aatacaagga ggcctcagaa
     1561 gttaagccac ccagagacat cactgctgag ttcctggtca agtctaagca cagggatctg
     1621 accgctctgt gcaaggaata cgacgaactg gctgagactc agggcaagct ggaggagaag
     1681 ctccaggagt tggaggccaa cccacccagt gacgtttacc tgtcttcccg tgaccgccag
     1741 atcctggatt ggcacttcgc caacttggag tttgctaatg ccaccccatt gagtaccctc
     1801 tctctcaagc actgggacca agatgatgac ttcgagttca ccggcagcca cttgactgtg
     1861 cgcaacggtt actcatgtgt tccagtcgcc ttggccgagg gcctcgacat taagttgaat
     1921 acagctgtca ggcaagtgag gtacactgct tctggctgtg aggtcatcgc tgttaacaca
     1981 cgcagcacat cccagacatt tatttacaaa tgtgacgctg tcctctgtac cttgccactg
     2041 ggcgtgctca aacagcaacc ccccgctgtg cagttcgtgc cccctctccc agagtggaag
     2101 accagcgccg tccagcgcat gggtttcgga aacctgaata aggtggtctt gtgctttgat
     2161 agggttttct gggatccttc cgtcaacctg ttcggccatg tgggttctac tactgcctct
     2221 cgcggagaac tgttcttgtt ttggaacctc tacaaagctc caattctctt ggccctcgtt
     2281 gccggcgagg ccgccggaat catggaaaac atctccgacg acgtgattgt tggccgttgt
     2341 ttggccatcc tgaagggcat cttcggatca tccgccgtgc cccaacctaa ggaaactgtg
     2401 gtgagccgct ggcgtgctga cccatgggct cgtggcagct actcatacgt cgctgctgga
     2461 tcttccggca atgactatga cctgatggct cagccaatca caccaggtcc tagcatccct
     2521 ggcgcccctc agcccatccc ccgcctcttt ttcgccggag aacacacaat ccgtaactac
     2581 ccagccacag tgcacggagc tctcctgtca ggactccgcg aggccggtcg tatcgccgac
     2641 cagttcctcg gcgccatgta cactctgcca cgccaagcca ctccaggtgt ccccgcccag
     2701 caaagccctt caatgtaagc tagcagctga tgcatagcat gcggtaccgg gagatggggg
     2761 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     2821 aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     2881 ccagggctgg cactctgtcg ataccccacc gagaccccat tgggaccaat acgcccgcgt
     2941 ttcttccttt tccccacccc aacccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     3001 cgtcggggcg gcaagccctg ccatagccac tacgggtacg tttaaaccca tgtgcctggc
     3061 agataacttc gtataatgta tgctatacga agttatggta cgtactaagc tctcatgttt
     3121 cacgtactaa gctctcatgt ttaacgtact aagctctcat gtttaacgaa ctaaaccctc
     3181 atggctaacg tactaagctc tcatggctaa cgtactaagc tctcatgttt cacgtactaa
     3241 gctctcatgt ttgaacaata aaattaatat aaatcagcaa cttaaatagc ctctaaggtt
     3301 ttaagtttta taagaaaaaa aagaatatat aaggctttta aagcttttaa ggtttaacgg
     3361 ttgtggacaa caagccaggg atgtaacgca ctgagaagcc cttagagcct ctcaaagcaa
     3421 ttttgagtga cacaggaaca cttaacggct gacatgggaa ttagcttcac gctgccgcaa
     3481 gcactcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca gtccgcagaa
     3541 acggtgctga ccccggatga atgtcagctg ggaggcagaa taaatgatca tatcgtcaat
     3601 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     3661 cacactgctt ccggtagtca ataaaccggt aagtagcgta tgcgctcacg caactggtcc
     3721 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     3781 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     3841 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     3901 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     3961 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     4021 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     4081 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     4141 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     4201 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     4261 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     4321 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     4381 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     4441 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     4501 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     4561 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     4621 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     4681 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacggatc
     4741 tatgtcgggt gcggagaaag aggtaatgaa atggcaccta ggtat
//
