LOCUS       sequence-233817-        4230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a human C-terminal LSD1 fragment to be expressed in a 
            baculovirus/insect cell expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Inui K, Zhao Z, Yuan J, Jayaprakash S, Le LTM, Drakulic S, Sander B,
            Golas MM
  TITLE     Stepwise assembly of functional C-terminal REST/NRSF transcriptional
            repressor complexes as a drug target.
  JOURNAL   Protein Sci. 2017 May;26(5):997-1011. doi: 10.1002/pro.3142. Epub 
            2017 Mar 12.
  PUBMED    28218430
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3142"; 
            journalName: "Protein Sci"; date: "2017-05"; volume: "26"; issue: 
            "5"; pages: "997-1011"
COMMENT     Sequence Label: pIDS-LSD1-C
FEATURES             Location/Qualifiers
     source          1..4230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..125
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     primer_bind     complement(2214..2233)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2258..2306
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    2508..2541
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      2543..2899
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             3364..4155
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgatactagt atacggacct ttaattcaac ccaacacaat atattatagt taaataagaa
       61 ttattatcaa atcatttgta tattaattaa aatactatac tgtaaattac attttattta
      121 caatcactcg acgaagactt gatcacccgg gatctcgaga tgccaagcgg tgtcgagggc
      181 gctgctttcc aaagccgttt gcctcatgat cgtatgacat cccaggaggc cgcctgcttc
      241 cctgacatca tcagcggccc ccaacagaca cagaaagttt tcctcttcat caggaacaga
      301 accctccaac tgtggctcga caacccaaag attcagctca ctttcgaggc tacactgcaa
      361 caactcgaag ctccttataa ctctgatact gtgctggtcc acagagtcca ctcttacctc
      421 gaacgccatg gtctgatcaa cttcggaatc tacaagcgca ttaagcccct gcctaccaaa
      481 aagactggta aagtgatcat catcggttca ggcgttagcg gcctggctgc tgcccgccag
      541 ctccaaagtt tcggtatgga cgtcaccctg ctggaggcta gggaccgcgt cggaggcagg
      601 gttgctacat tcaggaaggg caactacgtt gccgacctcg gcgccatggt tgttaccggc
      661 ctgggtggta accccatggc tgtggtgagc aagcaggtca acatggaact cgccaagatt
      721 aagcaaaagt gccctctcta cgaagctaat ggccaggccg tgccaaagga aaaggacgaa
      781 atggtggaac aggagttcaa ccgcctgctg gaggccacta gctacctctc ccaccagctg
      841 gacttcaacg tgctgaataa caaacctgtt agtctgggtc aagccctgga agtggttatc
      901 cagctgcaag agaagcacgt taaggacgag cagatcgaac actggaagaa gatcgttaag
      961 actcaagaag agctgaaaga actcctcaac aagatggtta acctgaagga aaaaatcaag
     1021 gagctgcacc aacaatacaa ggaggcctca gaagttaagc cacccagaga catcactgct
     1081 gagttcctgg tcaagtctaa gcacagggat ctgaccgctc tgtgcaagga atacgacgaa
     1141 ctggctgaga ctcagggcaa gctggaggag aagctccagg agttggaggc caacccaccc
     1201 agtgacgttt acctgtcttc ccgtgaccgc cagatcctgg attggcactt cgccaacttg
     1261 gagtttgcta atgccacccc attgagtacc ctctctctca agcactggga ccaagatgat
     1321 gacttcgagt tcaccggcag ccacttgact gtgcgcaacg gttactcatg tgttccagtc
     1381 gccttggccg agggcctcga cattaagttg aatacagctg tcaggcaagt gaggtacact
     1441 gcttctggct gtgaggtcat cgctgttaac acacgcagca catcccagac atttatttac
     1501 aaatgtgacg ctgtcctctg taccttgcca ctgggcgtgc tcaaacagca accccccgct
     1561 gtgcagttcg tgccccctct cccagagtgg aagaccagcg ccgtccagcg catgggtttc
     1621 ggaaacctga ataaggtggt cttgtgcttt gatagggttt tctgggatcc ttccgtcaac
     1681 ctgttcggcc atgtgggttc tactactgcc tctcgcggag aactgttctt gttttggaac
     1741 ctctacaaag ctccaattct cttggccctc gttgccggcg aggccgccgg aatcatggaa
     1801 aacatctccg acgacgtgat tgttggccgt tgtttggcca tcctgaaggg catcttcgga
     1861 tcatccgccg tgccccaacc taaggaaact gtggtgagcc gctggcgtgc tgacccatgg
     1921 gctcgtggca gctactcata cgtcgctgct ggatcttccg gcaatgacta tgacctgatg
     1981 gctcagccaa tcacaccagg tcctagcatc cctggcgccc ctcagcccat cccccgcctc
     2041 tttttcgccg gagaacacac aatccgtaac tacccagcca cagtgcacgg agctctcctg
     2101 tcaggactcc gcgaggccgg tcgtatcgcc gaccagttcc tcggcgccat gtacactctg
     2161 taagctagca gctgatgcat agcatgcggt accgggagat gggggaggct aactgaaaca
     2221 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     2281 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     2341 tgtcgatacc ccaccgagac cccattggga ccaatacgcc cgcgtttctt ccttttcccc
     2401 accccaaccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcaag
     2461 ccctgccata gccactacgg gtacgtttaa acccatgtgc ctggcagata acttcgtata
     2521 atgtatgcta tacgaagtta tggtacgtac taagctctca tgtttcacgt actaagctct
     2581 catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta
     2641 agctctcatg gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa
     2701 caataaaatt aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga
     2761 aaaaaaagaa tatataaggc ttttaaagct tttaaggttt aacggttgtg gacaacaagc
     2821 cagggatgta acgcactgag aagcccttag agcctctcaa agcaattttg agtgacacag
     2881 gaacacttaa cggctgacat gggaattagc ttcacgctgc cgcaagcact cagggcgcaa
     2941 gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt gctgaccccg
     3001 gatgaatgtc agctgggagg cagaataaat gatcatatcg tcaattatta cctccacggg
     3061 gagagcctga gcaaactggc ctcaggcatt tgagaagcac acggtcacac tgcttccggt
     3121 agtcaataaa ccggtaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     3181 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     3241 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     3301 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     3361 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     3421 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     3481 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     3541 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     3601 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     3661 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     3721 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     3781 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     3841 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     3901 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     3961 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     4021 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     4081 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     4141 gtagtcggca aataatgtct aacaattcgt tcaagccgac ggatctatgt cgggtgcgga
     4201 gaaagaggta atgaaatggc acctaggtat
//
