LOCUS       sequence-233844-       10157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-2 chain (Col1a2) with 
            mVenus between signal sequence and exon 6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10157)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: mVenus-pro-alpha-2(I)
FEATURES             Location/Qualifiers
     source          1..10157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(425..427,428..1138)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(467..488)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(728..747)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5134..5151)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5140..5364
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5410..5838
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5497..5516)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5707..5728
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5847..5867)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5852..6181
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6032..6167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6094..6113
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6248..7042
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6302..6321)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6912..6931
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7216..7337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7253..7272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7307..7326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7386..7402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7386..7402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7399..7421)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7410..7426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7434..7440,7441..7458,7459..7464))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7479..7500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7617..7634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7788..8376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7868..7887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8547..9338,9339..9407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9170..9189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9408..9512)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9587..9606)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9778..10157
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc
      301 ttggtaccga gctcggatcc actagtccag tgtggtggaa ttcgtagaca tgctcagctt
      361 tgtggatacg cggactctgt tgctgcttgc agtaacttcg tgcctagcaa catgccaaga
      421 tatcgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      481 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      541 cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac
      601 cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
      661 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      721 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      781 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      841 caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa
      901 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
      961 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1021 ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     1081 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagga
     1141 tatctattct gacaaaggag tttcatctgg ccctggacca atgggtttaa tgggacccag
     1201 aggccctcct ggtgctgttg gagcccctgg ccctcaaggt ttccaaggac ctgctggtga
     1261 acctggtgag cctggtcaaa cgggtcctgc aggtccccgt ggtccagctg gctctcctgg
     1321 caaggctggt gaggatggtc accctggaaa acccggaaga cctggggaga gaggagtcgt
     1381 tggaccacag ggtgctcgtg gtttccccgg gactcctgga cttcctggct tcaaaggcgt
     1441 gaaaggacac agtggtatgg atggattaaa gggacagcct ggtgcacagg gtgtgaaggg
     1501 tgaacccggt gcccctggag agaatggaac tccaggtcaa gcaggagccc gagggcttcc
     1561 tggtgagaga gggcgtgtcg gagctcctgg tccagctggt gcccgaggca gtgatggaag
     1621 cgttggcccc gtcggccctg ctggtcccat tgggtctgct ggaccccctg gtttcccagg
     1681 tgctcctggt cccaagggtg aacttgggcc cgttggtaac cctggccctg ctggtcctgc
     1741 tggtccccgt ggtgaagtgg gtcttccagg tctttctggc cctgttggac ctcctggcaa
     1801 ccctggaaca aatgggctca ctggcgccaa gggtgctact ggactccctg gtgtcgctgg
     1861 ggctcccggt ctccctggcc cccgtggtat tcctggcccc gctggtgctg ctggtgctac
     1921 aggtgccaga ggacttgttg gtgagcctgg tcctgctggc tccaaaggag aatccggtaa
     1981 caagggtgag cctggctctg ttggagccca aggtcctcct ggtcccagcg gtgaagaagg
     2041 aaagagaggg tctcccggag aagctggatc tgctggccct gcagggcctc cagggcttag
     2101 aggcagccct ggttctcgag gtcttcctgg agctgatggc agagctggtg taatgggtcc
     2161 tcctggcaat cgtggttcaa ccggccctgc tggaatccga ggtcctaatg gagatgccgg
     2221 tcgacctggg gaacctggtc tcatgggacc cagaggtctt cctgggtctc ctggaaatgt
     2281 tggcccatct ggtaaagaag gccctgtggg tctccctggc attgatggca gacctggccc
     2341 aatcggccca gctggaccaa gaggtgaagc tggcaacatt ggattccctg gacccaaagg
     2401 cccctctggt gatcctggca aacctggtga gagaggacac cccggtcttg ctggtgctcg
     2461 gggagctcca ggacccgatg gcaacaatgg agctcagggc ccccctggac cccagggtgt
     2521 tcaaggtggc aaaggtgaac aaggccctgc tggtcctcct ggcttccagg gtctccctgg
     2581 tccctcaggt actactggag aagttggcaa gcccggagaa aggggtcttc ctggtgaatt
     2641 cggtctccct ggtcctgctg gtccaagagg agaacgtggt accccgggtg agagtggagc
     2701 tgctggccct tctggtccta ttggaagccg aggtcccagt ggagccccag ggcctgatgg
     2761 aaacaagggt gaagctggtg cagtcggtgc tccaggcagt gctggtgcct ctgggcctgg
     2821 tgggcttcca ggagagaggg gtgctgctgg catacctggg ggcaaaggag aaaagggtga
     2881 aactggtctc cgaggtgaca ctggcaacac tggtagagat ggtgctcgtg gcattcctgg
     2941 tgctgtaggt gcccctggtc ctgctggggc ctcaggtgac cggggtgaag ctggtgctgc
     3001 cggtccttct ggcccagctg gtcctcgggg tagccctggt gaacgtggtg aagttggccc
     3061 tgctggcccc aatggatttg ctggtcctgc tggtgctgct ggccaacccg gtgctaaagg
     3121 agagaaggga accaaagggc ctaagggtga aaatggcatt gttggtccaa ccgggtctgt
     3181 tggagctgct ggcccatctg gtcctaatgg cccacctggt cctgttggaa gtcgtggtga
     3241 tggagggccc cctggtatga ctggcttccc tggtgctgct ggacggactg gtccccccgg
     3301 acccagtggt attgctggcc cccctggccc ccctggtgct gctggaaagg aagggattcg
     3361 tggtccccgt ggcgaccaag gtccagttgg ccgaactgga gaaacaggtg catctggacc
     3421 ccctggattt gttggtgaaa agggtccctc tggagaacct ggtaccgcag gagcccctgg
     3481 taccgcaggt cctcagggtc ttcttggtgc tcccggtatt ctgggtctcc ctggctctag
     3541 aggtgaacgt ggtctgccag gtattgctgg tgctctgggt gaacctggtc ctctgggcat
     3601 ctcaggccct cctggtgccc gtggtccccc tggtgctgtg ggcagccctg gagttaatgg
     3661 tgcccctggt gaagctggtc gtgatggtaa ccctggcagc gatggtcccc caggtcgtga
     3721 tggtcagcct ggacacaagg gagagcgtgg ttaccctggc agcattggtc cgactggtgc
     3781 tgcaggtgcc cctggtcctc acggttctgt gggtcctgct gggaaacatg gaaaccgagg
     3841 tgaacctggt cctgccggtt ctgttggtcc tgttggtgct gttggtccaa gaggtcctag
     3901 tggtcctcaa ggcatccgag gtgacaaagg agaacctggt gataaagggc acaggggtct
     3961 tcctggctta aaaggataca gtggattgca gggtcttcct ggtcttgctg gcctacatgg
     4021 tgaccaagga gctcctggtc ctgtgggtcc tgctggtccc aggggtcctg ctgggccttc
     4081 tggacccgtt ggcaaagatg gtagatctgg gcaacctggc ccagtcgggc ctgctggtgt
     4141 tcgtggttct cagggtagcc aaggtcccgc tggtccccct ggtccccctg gacctcctgg
     4201 tcctcctggt gtcagcggag gtggctatga ctttggtttt gaaggagact tctacagggc
     4261 tgaccagcct cgctcccagc cttcactcag acccaaggac tatgaagttg atgcaactct
     4321 gaaatctctc aataaccaga ttgagaccct tctcactcct gaaggctcta gaaagaaccc
     4381 tgctcgcacg tgccgggact taagactcag ccacccagag tggaacagcg attactactg
     4441 gattgaccct aaccaaggat gtactatgga tgccatcaaa gtgtactgtg atttctctac
     4501 tggtgaaacc tgcatccagg cccaacctgt aaacacccca gcgaagaact catacagccg
     4561 cgcccaggcc aacaagcatg tctggttagg agagactatc aatggtggca gccagtttga
     4621 atacaacgta gaaggggtgt cctccaagga aatggcaact cagctcgcct tcatgcgcct
     4681 gctagccaac cgtgcttctc agaacatcac ctaccactgc aagaacagca ttgcgtacct
     4741 ggatgaggag acgggcagct tgaacaaggc tgtgcttctg cagggttcca acgatgttga
     4801 acttgttgct gagggcaaca gcaggttcac ctactctgtc ctagtcgatg gctgctccaa
     4861 aaagacaaat gaatggggca agacaatcat tgaatacaaa acaaataagc catctcgcct
     4921 gccgttcctt gacattgcac ctctggacat tggtggtgct gaccaagaat tccgtgtgga
     4981 ggttggcccc gtctgtttca aataagtgaa ctcaacataa attaaaaacc aaaaacccct
     5041 gaaaaaactt tctctttgcc gtttcctcct ttaaaaaaaa aaaaaaaggg cggccgctcg
     5101 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     5161 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5221 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5281 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     5341 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     5401 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5461 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5521 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5581 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5641 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5701 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5761 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5821 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5881 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5941 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6001 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6061 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6121 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6181 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     6241 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     6301 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     6361 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     6421 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     6481 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     6541 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     6601 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     6661 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     6721 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     6781 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     6841 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     6901 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     6961 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     7021 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     7081 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     7141 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     7201 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7261 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7321 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     7381 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7441 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7501 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7561 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7621 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7681 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7741 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7801 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7861 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7921 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7981 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8041 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8101 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8161 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8221 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8281 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8341 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8401 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8461 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8521 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8581 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8641 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8701 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8761 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8821 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8881 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8941 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9001 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9061 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9121 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9181 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9241 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9301 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9361 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9421 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9481 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9541 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     9601 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     9661 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     9721 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     9781 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     9841 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     9901 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     9961 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
    10021 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
    10081 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
    10141 tcatcgctat taccatg
//
