LOCUS       sequence-233864-       10157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-2 chain (Col1a2) with GFP 
            between signal sequence and exon 6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10157)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: eGFP-pro-alpha-2(I)
FEATURES             Location/Qualifiers
     source          1..10157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(805..807,808..1518)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(847..868)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1108..1127)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5514..5531)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5520..5744
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5790..6218
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5877..5896)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6087..6108
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6227..6247)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6232..6561
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6412..6547
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6474..6493
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6628..7422
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6682..6701)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7292..7311
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7596..7717
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7633..7652)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7687..7706
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7766..7782)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7766..7782)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7779..7801)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7790..7806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7814..7820,7821..7838,7839..7844))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7859..7880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7997..8014)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8168..8756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8248..8267)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8927..9718,9719..9787))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9550..9569
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9788..9892)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9967..9986)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttcgtagaca tgctcagctt tgtggatacg cggactctgt tgctgcttgc agtaacttcg
      781 tgcctagcaa catgccaaga tatcgtgagc aagggcgagg agctgttcac cggggtggtg
      841 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      901 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      961 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     1021 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1081 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1141 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1201 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     1261 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     1321 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1381 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
     1441 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1501 atggacgagc tgtacaagga tatctattct gacaaaggag tttcatctgg ccctggacca
     1561 atgggtttaa tgggacccag aggccctcct ggtgctgttg gagcccctgg ccctcaaggt
     1621 ttccaaggac ctgctggtga acctggtgag cctggtcaaa cgggtcctgc aggtccccgt
     1681 ggtccagctg gctctcctgg caaggctggt gaggatggtc accctggaaa acccggaaga
     1741 cctggggaga gaggagtcgt tggaccacag ggtgctcgtg gtttccccgg gactcctgga
     1801 cttcctggct tcaaaggcgt gaaaggacac agtggtatgg atggattaaa gggacagcct
     1861 ggtgcacagg gtgtgaaggg tgaacccggt gcccctggag agaatggaac tccaggtcaa
     1921 gcaggagccc gagggcttcc tggtgagaga gggcgtgtcg gagctcctgg tccagctggt
     1981 gcccgaggca gtgatggaag cgttggcccc gtcggccctg ctggtcccat tgggtctgct
     2041 ggaccccctg gtttcccagg tgctcctggt cccaagggtg aacttgggcc cgttggtaac
     2101 cctggccctg ctggtcctgc tggtccccgt ggtgaagtgg gtcttccagg tctttctggc
     2161 cctgttggac ctcctggcaa ccctggaaca aatgggctca ctggcgccaa gggtgctact
     2221 ggactccctg gtgtcgctgg ggctcccggt ctccctggcc cccgtggtat tcctggcccc
     2281 gctggtgctg ctggtgctac aggtgccaga ggacttgttg gtgagcctgg tcctgctggc
     2341 tccaaaggag aatccggtaa caagggtgag cctggctctg ttggagccca aggtcctcct
     2401 ggtcccagcg gtgaagaagg aaagagaggg tctcccggag aagctggatc tgctggccct
     2461 gcagggcctc cagggcttag aggcagccct ggttctcgag gtcttcctgg agctgatggc
     2521 agagctggtg taatgggtcc tcctggcaat cgtggttcaa ccggccctgc tggaatccga
     2581 ggtcctaatg gagatgccgg tcgacctggg gaacctggtc tcatgggacc cagaggtctt
     2641 cctgggtctc ctggaaatgt tggcccatct ggtaaagaag gccctgtggg tctccctggc
     2701 attgatggca gacctggccc aatcggccca gctggaccaa gaggtgaagc tggcaacatt
     2761 ggattccctg gacccaaagg cccctctggt gatcctggca aacctggtga gagaggacac
     2821 cccggtcttg ctggtgctcg gggagctcca ggacccgatg gcaacaatgg agctcagggc
     2881 ccccctggac cccagggtgt tcaaggtggc aaaggtgaac aaggccctgc tggtcctcct
     2941 ggcttccagg gtctccctgg tccctcaggt actactggag aagttggcaa gcccggagaa
     3001 aggggtcttc ctggtgaatt cggtctccct ggtcctgctg gtccaagagg agaacgtggt
     3061 accccgggtg agagtggagc tgctggccct tctggtccta ttggaagccg aggtcccagt
     3121 ggagccccag ggcctgatgg aaacaagggt gaagctggtg cagtcggtgc tccaggcagt
     3181 gctggtgcct ctgggcctgg tgggcttcca ggagagaggg gtgctgctgg catacctggg
     3241 ggcaaaggag aaaagggtga aactggtctc cgaggtgaca ctggcaacac tggtagagat
     3301 ggtgctcgtg gcattcctgg tgctgtaggt gcccctggtc ctgctggggc ctcaggtgac
     3361 cggggtgaag ctggtgctgc cggtccttct ggcccagctg gtcctcgggg tagccctggt
     3421 gaacgtggtg aagttggccc tgctggcccc aatggatttg ctggtcctgc tggtgctgct
     3481 ggccaacccg gtgctaaagg agagaaggga accaaagggc ctaagggtga aaatggcatt
     3541 gttggtccaa ccgggtctgt tggagctgct ggcccatctg gtcctaatgg cccacctggt
     3601 cctgttggaa gtcgtggtga tggagggccc cctggtatga ctggcttccc tggtgctgct
     3661 ggacggactg gtccccccgg acccagtggt attgctggcc cccctggccc ccctggtgct
     3721 gctggaaagg aagggattcg tggtccccgt ggcgaccaag gtccagttgg ccgaactgga
     3781 gaaacaggtg catctggacc ccctggattt gttggtgaaa agggtccctc tggagaacct
     3841 ggtaccgcag gagcccctgg taccgcaggt cctcagggtc ttcttggtgc tcccggtatt
     3901 ctgggtctcc ctggctctag aggtgaacgt ggtctgccag gtattgctgg tgctctgggt
     3961 gaacctggtc ctctgggcat ctcaggccct cctggtgccc gtggtccccc tggtgctgtg
     4021 ggcagccctg gagttaatgg tgcccctggt gaagctggtc gtgatggtaa ccctggcagc
     4081 gatggtcccc caggtcgtga tggtcagcct ggacacaagg gagagcgtgg ttaccctggc
     4141 agcattggtc cgactggtgc tgcaggtgcc cctggtcctc acggttctgt gggtcctgct
     4201 gggaaacatg gaaaccgagg tgaacctggt cctgccggtt ctgttggtcc tgttggtgct
     4261 gttggtccaa gaggtcctag tggtcctcaa ggcatccgag gtgacaaagg agaacctggt
     4321 gataaagggc acaggggtct tcctggctta aaaggataca gtggattgca gggtcttcct
     4381 ggtcttgctg gcctacatgg tgaccaagga gctcctggtc ctgtgggtcc tgctggtccc
     4441 aggggtcctg ctgggccttc tggacccgtt ggcaaagatg gtagatctgg gcaacctggc
     4501 ccagtcgggc ctgctggtgt tcgtggttct cagggtagcc aaggtcccgc tggtccccct
     4561 ggtccccctg gacctcctgg tcctcctggt gtcagcggag gtggctatga ctttggtttt
     4621 gaaggagact tctacagggc tgaccagcct cgctcccagc cttcactcag acccaaggac
     4681 tatgaagttg atgcaactct gaaatctctc aataaccaga ttgagaccct tctcactcct
     4741 gaaggctcta gaaagaaccc tgctcgcacg tgccgggact taagactcag ccacccagag
     4801 tggaacagcg attactactg gattgaccct aaccaaggat gtactatgga tgccatcaaa
     4861 gtgtactgtg atttctctac tggtgaaacc tgcatccagg cccaacctgt aaacacccca
     4921 gcgaagaact catacagccg cgcccaggcc aacaagcatg tctggttagg agagactatc
     4981 aatggtggca gccagtttga atacaacgta gaaggggtgt cctccaagga aatggcaact
     5041 cagctcgcct tcatgcgcct gctagccaac cgtgcttctc agaacatcac ctaccactgc
     5101 aagaacagca ttgcgtacct ggatgaggag acgggcagct tgaacaaggc tgtgcttctg
     5161 cagggttcca acgatgttga acttgttgct gagggcaaca gcaggttcac ctactctgtc
     5221 ctagtcgatg gctgctccaa aaagacaaat gaatggggca agacaatcat tgaatacaaa
     5281 acaaataagc catctcgcct gccgttcctt gacattgcac ctctggacat tggtggtgct
     5341 gaccaagaat tccgtgtgga ggttggcccc gtctgtttca aataagtgaa ctcaacataa
     5401 attaaaaacc aaaaacccct gaaaaaactt tctctttgcc gtttcctcct ttaaaaaaaa
     5461 aaaaaaaggg cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     5521 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5581 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5641 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5701 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5761 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     5821 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5881 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5941 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6001 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6061 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6121 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6181 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     6241 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6301 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6361 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6421 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6481 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     6541 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     6601 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     6661 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     6721 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     6781 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     6841 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     6901 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     6961 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     7021 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     7081 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     7141 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     7201 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     7261 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     7321 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     7381 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     7441 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     7501 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     7561 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     7621 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7681 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     7741 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7801 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7861 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7921 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7981 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8041 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8101 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8161 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8221 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8281 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8341 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8401 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8461 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8521 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8581 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8641 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8701 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8761 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8821 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8881 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8941 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9001 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9061 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9121 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9181 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9241 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9301 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9361 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9421 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9481 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9541 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9601 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9661 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9721 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9781 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9841 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9901 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     9961 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
    10021 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
    10081 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
    10141 ggccagatat acgcgtt
//
