LOCUS       sequence-233865-       10148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-2 chain (Col1a2) with 
            mApple between signal sequence and exon 6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: mApple-pro-alpha-2(I)
FEATURES             Location/Qualifiers
     source          1..10148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             805..1509
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAF
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFE
                     DGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALKS
                     EIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     1215..1234
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5505..5522)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5511..5735
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5781..6209
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5868..5887)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6078..6099
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6218..6238)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6223..6552
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6403..6538
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6465..6484
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6619..7413
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6673..6692)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7283..7302
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7587..7708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7624..7643)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7678..7697
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7757..7773)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7757..7773)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7770..7792)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7781..7797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7805..7811,7812..7829,7830..7835))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7850..7871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7988..8005)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8159..8747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8239..8258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8918..9709,9710..9778))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9541..9560
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9779..9883)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9958..9977)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttcgtagaca tgctcagctt tgtggatacg cggactctgt tgctgcttgc agtaacttcg
      781 tgcctagcaa catgccaaga tatcgtgagc aagggcgagg agaataacat ggccatcatc
      841 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      901 atcgagggcg agggcgaggg ccgcccctac gaggcctttc agaccgctaa gctgaaggtg
      961 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     1021 tccaaggtct acattaagca cccagccgac atccccgact acttcaagct gtccttcccc
     1081 gagggcttca ggtgggagcg cgtgatgaac ttcgaggacg gcggcattat tcacgttaac
     1141 caggactcct ccctgcagga cggcgtgttc atctacaagg tgaagctgcg cggcaccaac
     1201 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccgaggag
     1261 cggatgtacc ccgaggacgg cgccctgaag agcgagatca agaagaggct gaagctgaag
     1321 gacggcggcc actacgccgc cgaggtcaag accacctaca aggccaagaa gcccgtgcag
     1381 ctgcccggcg cctacatcgt cgacatcaag ttggacatcg tgtcccacaa cgaggactac
     1441 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     1501 ctgtacaagg atatctattc tgacaaagga gtttcatctg gccctggacc aatgggttta
     1561 atgggaccca gaggccctcc tggtgctgtt ggagcccctg gccctcaagg tttccaagga
     1621 cctgctggtg aacctggtga gcctggtcaa acgggtcctg caggtccccg tggtccagct
     1681 ggctctcctg gcaaggctgg tgaggatggt caccctggaa aacccggaag acctggggag
     1741 agaggagtcg ttggaccaca gggtgctcgt ggtttccccg ggactcctgg acttcctggc
     1801 ttcaaaggcg tgaaaggaca cagtggtatg gatggattaa agggacagcc tggtgcacag
     1861 ggtgtgaagg gtgaacccgg tgcccctgga gagaatggaa ctccaggtca agcaggagcc
     1921 cgagggcttc ctggtgagag agggcgtgtc ggagctcctg gtccagctgg tgcccgaggc
     1981 agtgatggaa gcgttggccc cgtcggccct gctggtccca ttgggtctgc tggaccccct
     2041 ggtttcccag gtgctcctgg tcccaagggt gaacttgggc ccgttggtaa ccctggccct
     2101 gctggtcctg ctggtccccg tggtgaagtg ggtcttccag gtctttctgg ccctgttgga
     2161 cctcctggca accctggaac aaatgggctc actggcgcca agggtgctac tggactccct
     2221 ggtgtcgctg gggctcccgg tctccctggc ccccgtggta ttcctggccc cgctggtgct
     2281 gctggtgcta caggtgccag aggacttgtt ggtgagcctg gtcctgctgg ctccaaagga
     2341 gaatccggta acaagggtga gcctggctct gttggagccc aaggtcctcc tggtcccagc
     2401 ggtgaagaag gaaagagagg gtctcccgga gaagctggat ctgctggccc tgcagggcct
     2461 ccagggctta gaggcagccc tggttctcga ggtcttcctg gagctgatgg cagagctggt
     2521 gtaatgggtc ctcctggcaa tcgtggttca accggccctg ctggaatccg aggtcctaat
     2581 ggagatgccg gtcgacctgg ggaacctggt ctcatgggac ccagaggtct tcctgggtct
     2641 cctggaaatg ttggcccatc tggtaaagaa ggccctgtgg gtctccctgg cattgatggc
     2701 agacctggcc caatcggccc agctggacca agaggtgaag ctggcaacat tggattccct
     2761 ggacccaaag gcccctctgg tgatcctggc aaacctggtg agagaggaca ccccggtctt
     2821 gctggtgctc ggggagctcc aggacccgat ggcaacaatg gagctcaggg cccccctgga
     2881 ccccagggtg ttcaaggtgg caaaggtgaa caaggccctg ctggtcctcc tggcttccag
     2941 ggtctccctg gtccctcagg tactactgga gaagttggca agcccggaga aaggggtctt
     3001 cctggtgaat tcggtctccc tggtcctgct ggtccaagag gagaacgtgg taccccgggt
     3061 gagagtggag ctgctggccc ttctggtcct attggaagcc gaggtcccag tggagcccca
     3121 gggcctgatg gaaacaaggg tgaagctggt gcagtcggtg ctccaggcag tgctggtgcc
     3181 tctgggcctg gtgggcttcc aggagagagg ggtgctgctg gcatacctgg gggcaaagga
     3241 gaaaagggtg aaactggtct ccgaggtgac actggcaaca ctggtagaga tggtgctcgt
     3301 ggcattcctg gtgctgtagg tgcccctggt cctgctgggg cctcaggtga ccggggtgaa
     3361 gctggtgctg ccggtccttc tggcccagct ggtcctcggg gtagccctgg tgaacgtggt
     3421 gaagttggcc ctgctggccc caatggattt gctggtcctg ctggtgctgc tggccaaccc
     3481 ggtgctaaag gagagaaggg aaccaaaggg cctaagggtg aaaatggcat tgttggtcca
     3541 accgggtctg ttggagctgc tggcccatct ggtcctaatg gcccacctgg tcctgttgga
     3601 agtcgtggtg atggagggcc ccctggtatg actggcttcc ctggtgctgc tggacggact
     3661 ggtccccccg gacccagtgg tattgctggc ccccctggcc cccctggtgc tgctggaaag
     3721 gaagggattc gtggtccccg tggcgaccaa ggtccagttg gccgaactgg agaaacaggt
     3781 gcatctggac cccctggatt tgttggtgaa aagggtccct ctggagaacc tggtaccgca
     3841 ggagcccctg gtaccgcagg tcctcagggt cttcttggtg ctcccggtat tctgggtctc
     3901 cctggctcta gaggtgaacg tggtctgcca ggtattgctg gtgctctggg tgaacctggt
     3961 cctctgggca tctcaggccc tcctggtgcc cgtggtcccc ctggtgctgt gggcagccct
     4021 ggagttaatg gtgcccctgg tgaagctggt cgtgatggta accctggcag cgatggtccc
     4081 ccaggtcgtg atggtcagcc tggacacaag ggagagcgtg gttaccctgg cagcattggt
     4141 ccgactggtg ctgcaggtgc ccctggtcct cacggttctg tgggtcctgc tgggaaacat
     4201 ggaaaccgag gtgaacctgg tcctgccggt tctgttggtc ctgttggtgc tgttggtcca
     4261 agaggtccta gtggtcctca aggcatccga ggtgacaaag gagaacctgg tgataaaggg
     4321 cacaggggtc ttcctggctt aaaaggatac agtggattgc agggtcttcc tggtcttgct
     4381 ggcctacatg gtgaccaagg agctcctggt cctgtgggtc ctgctggtcc caggggtcct
     4441 gctgggcctt ctggacccgt tggcaaagat ggtagatctg ggcaacctgg cccagtcggg
     4501 cctgctggtg ttcgtggttc tcagggtagc caaggtcccg ctggtccccc tggtccccct
     4561 ggacctcctg gtcctcctgg tgtcagcgga ggtggctatg actttggttt tgaaggagac
     4621 ttctacaggg ctgaccagcc tcgctcccag ccttcactca gacccaagga ctatgaagtt
     4681 gatgcaactc tgaaatctct caataaccag attgagaccc ttctcactcc tgaaggctct
     4741 agaaagaacc ctgctcgcac gtgccgggac ttaagactca gccacccaga gtggaacagc
     4801 gattactact ggattgaccc taaccaagga tgtactatgg atgccatcaa agtgtactgt
     4861 gatttctcta ctggtgaaac ctgcatccag gcccaacctg taaacacccc agcgaagaac
     4921 tcatacagcc gcgcccaggc caacaagcat gtctggttag gagagactat caatggtggc
     4981 agccagtttg aatacaacgt agaaggggtg tcctccaagg aaatggcaac tcagctcgcc
     5041 ttcatgcgcc tgctagccaa ccgtgcttct cagaacatca cctaccactg caagaacagc
     5101 attgcgtacc tggatgagga gacgggcagc ttgaacaagg ctgtgcttct gcagggttcc
     5161 aacgatgttg aacttgttgc tgagggcaac agcaggttca cctactctgt cctagtcgat
     5221 ggctgctcca aaaagacaaa tgaatggggc aagacaatca ttgaatacaa aacaaataag
     5281 ccatctcgcc tgccgttcct tgacattgca cctctggaca ttggtggtgc tgaccaagaa
     5341 ttccgtgtgg aggttggccc cgtctgtttc aaataagtga actcaacata aattaaaaac
     5401 caaaaacccc tgaaaaaact ttctctttgc cgtttcctcc tttaaaaaaa aaaaaaaagg
     5461 gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     5521 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     5581 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     5641 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     5701 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     5761 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5821 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5881 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5941 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6001 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6061 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     6121 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6181 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     6241 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6301 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6361 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     6421 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6481 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6541 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     6601 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     6661 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     6721 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     6781 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     6841 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     6901 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     6961 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     7021 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     7081 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     7141 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     7201 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     7261 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     7321 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     7381 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     7441 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     7501 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     7561 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     7621 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7681 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     7741 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7801 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7861 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7921 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7981 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8041 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8101 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8161 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8221 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8281 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8341 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8401 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8461 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8521 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8581 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8641 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8701 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8761 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8821 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8881 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9001 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9061 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9301 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9361 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9901 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     9961 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
    10021 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
    10081 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
    10141 tacgcgtt
//
