LOCUS       sequence-233868-       10147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-2 chain (Col1a2) with 
            Gly610Cys mutation and mApple between signal sequence and exon 6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10147)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: mApple-pro-alpha-2(I)-G610C
FEATURES             Location/Qualifiers
     source          1..10147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             805..1509
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAF
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFE
                     DGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALKS
                     EIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     1215..1234
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5504..5521)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5510..5734
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5780..6208
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5867..5886)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6077..6098
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6217..6237)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6222..6551
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6402..6537
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6464..6483
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6618..7412
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6672..6691)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7282..7301
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7586..7707
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7623..7642)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7677..7696
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7756..7772)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7756..7772)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7769..7791)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7780..7796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7804..7810,7811..7828,7829..7834))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7849..7870
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7987..8004)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8158..8746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8238..8257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8917..9708,9709..9777))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9540..9559
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9778..9882)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9957..9976)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttcgtagaca tgctcagctt tgtggatacg cggactctgt tgctgcttgc agtaacttcg
      781 tgcctagcaa catgccaaga tatcgtgagc aagggcgagg agaataacat ggccatcatc
      841 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      901 atcgagggcg agggcgaggg ccgcccctac gaggcctttc agaccgctaa gctgaaggtg
      961 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     1021 tccaaggtct acattaagca cccagccgac atccccgact acttcaagct gtccttcccc
     1081 gagggcttca ggtgggagcg cgtgatgaac ttcgaggacg gcggcattat tcacgttaac
     1141 caggactcct ccctgcagga cggcgtgttc atctacaagg tgaagctgcg cggcaccaac
     1201 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccgaggag
     1261 cggatgtacc ccgaggacgg cgccctgaag agcgagatca agaagaggct gaagctgaag
     1321 gacggcggcc actacgccgc cgaggtcaag accacctaca aggccaagaa gcccgtgcag
     1381 ctgcccggcg cctacatcgt cgacatcaag ttggacatcg tgtcccacaa cgaggactac
     1441 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     1501 ctgtacaagg atatctattc tgacaaagga gtttcatctg gccctggacc aatgggttta
     1561 atgggaccca gaggccctcc tggtgctgtt ggagcccctg gccctcaagg tttccaagga
     1621 cctgctggtg aacctggtga gcctggtcaa acgggtcctg caggtccccg tggtccagct
     1681 ggctctcctg gcaaggctgg tgaggatggt caccctggaa aacccggaag acctggggag
     1741 agaggagtcg ttggaccaca gggtgctcgt ggtttccccg ggactcctgg acttcctggc
     1801 ttcaaaggcg tgaaaggaca cagtggtatg gatggattaa agggacagcc tggtgcacag
     1861 ggtgtgaagg gtgaacccgg tgcccctgga gagaatggaa ctccaggtca agcaggagcc
     1921 cgagggcttc ctggtgagag agggcgtgtc ggagctcctg gtccagctgg tgcccgaggc
     1981 agtgatggaa gcgttggccc cgtcggccct gctggtccca ttgggtctgc tggaccccct
     2041 ggtttcccag gtgctcctgg tcccaagggt gaacttgggc ccgttggtaa ccctggccct
     2101 gctggtcctg ctggtccccg tggtgaagtg ggtcttccag gtctttctgg ccctgttgga
     2161 cctcctggca accctggaac aaatgggctc actggcgcca agggtgctac tggactccct
     2221 ggtgtcgctg gggctcccgg tctccctggc ccccgtggta ttcctggccc cgctggtgct
     2281 gctggtgcta caggtgccag aggacttgtt ggtgagcctg gtcctgctgg ctccaaagga
     2341 gaatccggta acaagggtga gcctggctct gttggagccc aaggtcctcc tggtcccagc
     2401 ggtgaagaag gaaagagagg gtctcccgga gaagctggat ctgctggccc tgcagggcct
     2461 ccagggctta gaggcagccc tggttctcga ggtcttcctg gagctgatgg cagagctggt
     2521 gtaatgggtc ctcctggcaa tcgtggttca accggccctg ctggaatccg aggtcctaat
     2581 ggagatgccg gtcgacctgg ggaacctggt ctcatgggac ccagaggtct tcctgggtct
     2641 cctggaaatg ttggcccatc tggtaaagaa ggccctgtgg gtctccctgg cattgatggc
     2701 agacctggcc caatcggccc agctggacca agaggtgaag ctggcaacat tggattccct
     2761 ggacccaaag gcccctctgg tgatcctggc aaacctggtg agagaggaca ccccggtctt
     2821 gctggtgctc ggggagctcc aggacccgat ggcaacaatg gagctcaggg cccccctgga
     2881 ccccagggtg ttcaaggtgg caaaggtgaa caaggccctg ctggtcctcc tggcttccag
     2941 ggtctccctg gtccctcagg tactactgga gaagttggca agcccggaga aaggggtctt
     3001 cctggtgaat tcggtctccc tggtcctgct ggtccaagag gagaacgtgg taccccgggt
     3061 gagagtggag ctgctggccc ttctggtcct attggaagcc gaggtcccag tggagcccca
     3121 gggcctgatg gaaacaaggg tgaagctggt gcagtcggtg ctccaggcag tgctggtgcc
     3181 tctgggcctg gtgggcttcc aggagagagg ggtgctgctg gcatacctgg gggcaaagga
     3241 gaaaagggtg aaactggtct ccgaggtgac actggcaaca ctggtagaga tggtgctcgt
     3301 ggcattcctg gtgctgtagg tgcccctggt cctgctgggg cctcaggtga ccggggtgaa
     3361 gctggtgctg cctgtccttc tggcccagct ggtcctcggg gtagccctgg tgaacgtggt
     3421 gaagttggcc ctgctggccc caatggattt gctggtcctg ctggtgctgc tggccaaccc
     3481 ggtgctaaag gagagaaggg aaccaaaggg cctaagggtg aaaatggcat tgttggtcca
     3541 accgggtctg ttggagctgc tggcccatct ggtcctaatg gcccacctgg tcctgttgga
     3601 agtcgtggtg atggagggcc ccctggtatg actggcttcc ctggtgctgc tggacggact
     3661 ggtccccccg gacccagtgg tattgctggc ccccctggcc cccctggtgc tgctggaaag
     3721 gaagggattc gtggtccccg tggcgaccaa ggtccagttg gccgaactgg agaaacaggt
     3781 gcatctggac cccctggatt tgttggtgaa aagggtccct ctggagaacc tggtaccgca
     3841 ggagcccctg gtaccgcagg tcctcagggt cttcttggtg ctcccggtat tctgggtctc
     3901 cctggctcta gaggtgaacg tggtctgcca ggtattgctg gtgctctggg tgaacctggt
     3961 cctctgggca tctcaggccc tcctggtgcc cgtggtcccc ctggtgctgt gggcagccct
     4021 ggagttaatg gtgcccctgg tgaagctggt cgtgatggta accctggcag cgatggtccc
     4081 ccaggtcgtg atggtcagcc tggacacaag ggagagcgtg gttaccctgg cagcattggt
     4141 ccgactggtg ctgcaggtgc ccctggtcct cacggttctg tgggtcctgc tgggaaacat
     4201 ggaaaccgag gtgaacctgg tcctgccggt tctgttggtc ctgttggtgc tgttggtcca
     4261 agaggtccta gtggtcctca aggcatccga ggtgacaaag gagaacctgg tgataaaggg
     4321 cacaggggtc ttcctggctt aaaaggatac agtggattgc agggtcttcc tggtcttgct
     4381 ggcctacatg gtgaccaagg agctcctggt cctgtgggtc ctgctggtcc caggggtcct
     4441 gctgggcctt ctggacccgt tggcaaagat ggtagatctg ggcaacctgg cccagtcggg
     4501 cctgctggtg ttcgtggttc tcagggtagc caaggtcccg ctggtccccc tggtccccct
     4561 ggacctcctg gtcctcctgg tgtcagcgga ggtggctatg actttggttt tgaaggagac
     4621 ttctacaggg ctgaccagcc tcgctcccag ccttcactca gacccaagga ctatgaagtt
     4681 gatgcaactc tgaaatctct caataaccag attgagaccc ttctcactcc tgaaggctct
     4741 agaaagaacc ctgctcgcac gtgccgggac ttaagactca gccacccaga gtggaacagc
     4801 gattactact ggattgaccc taaccaagga tgtactatgg atgccatcaa agtgtactgt
     4861 gatttctcta ctggtgaaac ctgcatccag gcccaacctg taaacacccc agcgaagaac
     4921 tcatacagcc gcgcccaggc caacaagcat gtctggttag gagagactat caatggtggc
     4981 agccagtttg aatacaacgt agaaggggtg tcctccaagg aaatggcaac tcagctcgcc
     5041 ttcatgcgcc tgctagccaa ccgtgcttct cagaacatca cctaccactg caagaacagc
     5101 attgcgtacc tggatgagga gacgggcagc ttgaacaagg ctgtgcttct gcagggttcc
     5161 aacgatgttg aacttgttgc tgagggcaac agcaggttca cctactctgt cctagtcgat
     5221 ggctgctcca aaaagacaaa tgaatggggc aagacaatca ttgaatacaa aacaaataag
     5281 ccatctcgcc tgccgttcct tgacattgca cctctggaca ttggtggtgc tgaccaagaa
     5341 ttccgtgtgg aggttggccc cgtctgtttc aaataagtga actcaacata aattaaaaac
     5401 caaaaacccc tgaaaaaact ttctctttgc cgtttcctcc tttaaaaaaa aaaaaaaggg
     5461 cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     5521 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5581 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5641 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     5701 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     5761 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5821 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5881 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5941 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6001 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6061 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6121 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6181 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     6241 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6301 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6361 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6421 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6481 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6541 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     6601 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     6661 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     6721 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6781 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     6841 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6901 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6961 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     7021 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     7081 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     7141 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     7201 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     7261 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     7321 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     7381 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     7441 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     7501 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     7561 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     7621 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7681 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     7741 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7801 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7861 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7921 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7981 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8041 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8101 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8161 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8221 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8281 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8341 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8401 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8461 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8521 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8581 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8641 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8701 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8761 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8821 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8881 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8941 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9001 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9061 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9121 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9181 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9241 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9301 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9361 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9421 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9481 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9541 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9601 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9661 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9721 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9781 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9841 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9901 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     9961 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
    10021 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
    10081 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
    10141 acgcgtt
//
