LOCUS       sequence-233870-       10313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-2 chain (Col1a2) with eGFP 
            replacing exon 2-3 retaining N-propeptide minor triple helix and 
            N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10313)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: eGFP-pro-alpha-2(I)-L
FEATURES             Location/Qualifiers
     source          1..10313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(805..807,808..1518)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(847..868)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1108..1127)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5670..5687)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5676..5900
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5946..6374
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6033..6052)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6243..6264
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6383..6403)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6388..6717
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6568..6703
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6630..6649
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6784..7578
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6838..6857)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7448..7467
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7752..7873
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7789..7808)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7843..7862
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7922..7938)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7922..7938)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7935..7957)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7946..7962
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7970..7976,7977..7994,7995..8000))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8015..8036
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8153..8170)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8324..8912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8404..8423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9083..9874,9875..9943))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9706..9725
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9944..10048)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10123..10142)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttcgtagaca tgctcagctt tgtggatacg cggactctgt tgctgcttgc agtaacttcg
      781 tgcctagcaa catgccaaga tatcgtgagc aagggcgagg agctgttcac cggggtggtg
      841 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      901 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      961 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     1021 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1081 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1141 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1201 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     1261 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     1321 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1381 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
     1441 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1501 atggacgagc tgtacaaggg ccccactgga gacagaggac cacgtggaca aaggggtccc
     1561 gcaggtcccc gaggcagaga tggtgttgat ggtcccatgg gccctcctgg tccccctggc
     1621 tcccctggtc ctcccggctc ccctgccccc cctggtctta ctgggaactt tgctgctcag
     1681 tattctgaca aaggagtttc atctggccct ggaccaatgg gtttaatggg acccagaggc
     1741 cctcctggtg ctgttggagc ccctggccct caaggtttcc aaggacctgc tggtgaacct
     1801 ggtgagcctg gtcaaacggg tcctgcaggt ccccgtggtc cagctggctc tcctggcaag
     1861 gctggtgagg atggtcaccc tggaaaaccc ggaagacctg gggagagagg agtcgttgga
     1921 ccacagggtg ctcgtggttt ccccgggact cctggacttc ctggcttcaa aggcgtgaaa
     1981 ggacacagtg gtatggatgg attaaaggga cagcctggtg cacagggtgt gaagggtgaa
     2041 cccggtgccc ctggagagaa tggaactcca ggtcaagcag gagcccgagg gcttcctggt
     2101 gagagagggc gtgtcggagc tcctggtcca gctggtgccc gaggcagtga tggaagcgtt
     2161 ggccccgtcg gccctgctgg tcccattggg tctgctggac cccctggttt cccaggtgct
     2221 cctggtccca agggtgaact tgggcccgtt ggtaaccctg gccctgctgg tcctgctggt
     2281 ccccgtggtg aagtgggtct tccaggtctt tctggccctg ttggacctcc tggcaaccct
     2341 ggaacaaatg ggctcactgg cgccaagggt gctactggac tccctggtgt cgctggggct
     2401 cccggtctcc ctggcccccg tggtattcct ggccccgctg gtgctgctgg tgctacaggt
     2461 gccagaggac ttgttggtga gcctggtcct gctggctcca aaggagaatc cggtaacaag
     2521 ggtgagcctg gctctgttgg agcccaaggt cctcctggtc ccagcggtga agaaggaaag
     2581 agagggtctc ccggagaagc tggatctgct ggccctgcag ggcctccagg gcttagaggc
     2641 agccctggtt ctcgaggtct tcctggagct gatggcagag ctggtgtaat gggtcctcct
     2701 ggcaatcgtg gttcaaccgg ccctgctgga atccgaggtc ctaatggaga tgccggtcga
     2761 cctggggaac ctggtctcat gggacccaga ggtcttcctg ggtctcctgg aaatgttggc
     2821 ccatctggta aagaaggccc tgtgggtctc cctggcattg atggcagacc tggcccaatc
     2881 ggcccagctg gaccaagagg tgaagctggc aacattggat tccctggacc caaaggcccc
     2941 tctggtgatc ctggcaaacc tggtgagaga ggacaccccg gtcttgctgg tgctcgggga
     3001 gctccaggac ccgatggcaa caatggagct cagggccccc ctggacccca gggtgttcaa
     3061 ggtggcaaag gtgaacaagg ccctgctggt cctcctggct tccagggtct ccctggtccc
     3121 tcaggtacta ctggagaagt tggcaagccc ggagaaaggg gtcttcctgg tgaattcggt
     3181 ctccctggtc ctgctggtcc aagaggagaa cgtggtaccc cgggtgagag tggagctgct
     3241 ggcccttctg gtcctattgg aagccgaggt cccagtggag ccccagggcc tgatggaaac
     3301 aagggtgaag ctggtgcagt cggtgctcca ggcagtgctg gtgcctctgg gcctggtggg
     3361 cttccaggag agaggggtgc tgctggcata cctgggggca aaggagaaaa gggtgaaact
     3421 ggtctccgag gtgacactgg caacactggt agagatggtg ctcgtggcat tcctggtgct
     3481 gtaggtgccc ctggtcctgc tggggcctca ggtgaccggg gtgaagctgg tgctgccggt
     3541 ccttctggcc cagctggtcc tcggggtagc cctggtgaac gtggtgaagt tggccctgct
     3601 ggccccaatg gatttgctgg tcctgctggt gctgctggcc aacccggtgc taaaggagag
     3661 aagggaacca aagggcctaa gggtgaaaat ggcattgttg gtccaaccgg gtctgttgga
     3721 gctgctggcc catctggtcc taatggccca cctggtcctg ttggaagtcg tggtgatgga
     3781 gggccccctg gtatgactgg cttccctggt gctgctggac ggactggtcc ccccggaccc
     3841 agtggtattg ctggcccccc tggcccccct ggtgctgctg gaaaggaagg gattcgtggt
     3901 ccccgtggcg accaaggtcc agttggccga actggagaaa caggtgcatc tggaccccct
     3961 ggatttgttg gtgaaaaggg tccctctgga gaacctggta ccgcaggagc ccctggtacc
     4021 gcaggtcctc agggtcttct tggtgctccc ggtattctgg gtctccctgg ctctagaggt
     4081 gaacgtggtc tgccaggtat tgctggtgct ctgggtgaac ctggtcctct gggcatctca
     4141 ggccctcctg gtgcccgtgg tccccctggt gctgtgggca gccctggagt taatggtgcc
     4201 cctggtgaag ctggtcgtga tggtaaccct ggcagcgatg gtcccccagg tcgtgatggt
     4261 cagcctggac acaagggaga gcgtggttac cctggcagca ttggtccgac tggtgctgca
     4321 ggtgcccctg gtcctcacgg ttctgtgggt cctgctggga aacatggaaa ccgaggtgaa
     4381 cctggtcctg ccggttctgt tggtcctgtt ggtgctgttg gtccaagagg tcctagtggt
     4441 cctcaaggca tccgaggtga caaaggagaa cctggtgata aagggcacag gggtcttcct
     4501 ggcttaaaag gatacagtgg attgcagggt cttcctggtc ttgctggcct acatggtgac
     4561 caaggagctc ctggtcctgt gggtcctgct ggtcccaggg gtcctgctgg gccttctgga
     4621 cccgttggca aagatggtag atctgggcaa cctggcccag tcgggcctgc tggtgttcgt
     4681 ggttctcagg gtagccaagg tcccgctggt ccccctggtc cccctggacc tcctggtcct
     4741 cctggtgtca gcggaggtgg ctatgacttt ggttttgaag gagacttcta cagggctgac
     4801 cagcctcgct cccagccttc actcagaccc aaggactatg aagttgatgc aactctgaaa
     4861 tctctcaata accagattga gacccttctc actcctgaag gctctagaaa gaaccctgct
     4921 cgcacgtgcc gggacttaag actcagccac ccagagtgga acagcgatta ctactggatt
     4981 gaccctaacc aaggatgtac tatggatgcc atcaaagtgt actgtgattt ctctactggt
     5041 gaaacctgca tccaggccca acctgtaaac accccagcga agaactcata cagccgcgcc
     5101 caggccaaca agcatgtctg gttaggagag actatcaatg gtggcagcca gtttgaatac
     5161 aacgtagaag gggtgtcctc caaggaaatg gcaactcagc tcgccttcat gcgcctgcta
     5221 gccaaccgtg cttctcagaa catcacctac cactgcaaga acagcattgc gtacctggat
     5281 gaggagacgg gcagcttgaa caaggctgtg cttctgcagg gttccaacga tgttgaactt
     5341 gttgctgagg gcaacagcag gttcacctac tctgtcctag tcgatggctg ctccaaaaag
     5401 acaaatgaat ggggcaagac aatcattgaa tacaaaacaa ataagccatc tcgcctgccg
     5461 ttccttgaca ttgcacctct ggacattggt ggtgctgacc aagaattccg tgtggaggtt
     5521 ggccccgtct gtttcaaata agtgaactca acataaatta aaaaccaaaa acccctgaaa
     5581 aaactttctc tttgccgttt cctcctttaa aaaaaaaaaa aaagggcggc cgctcgagtc
     5641 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5701 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5761 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5821 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     5881 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     5941 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6001 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6061 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6121 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6181 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6241 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6301 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6361 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     6421 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     6481 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6541 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6601 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     6661 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6721 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     6781 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     6841 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     6901 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     6961 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     7021 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     7081 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     7141 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     7201 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     7261 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     7321 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     7381 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     7441 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     7501 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     7561 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     7621 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     7681 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     7741 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7801 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7861 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     7921 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7981 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8041 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8101 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8161 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8221 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8281 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8341 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8401 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8461 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8521 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8581 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8641 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8701 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8761 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8821 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8881 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8941 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9001 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9061 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9121 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9181 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9241 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9301 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9361 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9421 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9481 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9541 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9601 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9661 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9721 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9781 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9841 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9901 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9961 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10021 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    10081 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    10141 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
    10201 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
    10261 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gtt
//
