LOCUS       sequence-233871-       10511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-1 chain (Col1a1) with 
            mVenus replacing exon 2-3 retaining N-propeptide minor triple helix 
            and N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: mVenus-pro-alpha-1(I)
FEATURES             Location/Qualifiers
     source          1..10511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(398..400,401..1108)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(440..461)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(701..720)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1048..1069
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5488..5505)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5494..5718
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5764..6192
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5851..5870)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6061..6082
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6201..6221)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6206..6535
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6386..6521
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6448..6467
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6602..7396
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6656..6675)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7266..7285
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7570..7691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7607..7626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7661..7680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7740..7756)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7740..7756)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7753..7775)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7764..7780
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7788..7794,7795..7812,7813..7818))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7833..7854
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7971..7988)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8142..8730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8222..8241)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8901..9692,9693..9761))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9524..9543
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9762..9866)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9941..9960)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10132..10511
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc
      301 ttatgtctag ggtctagaca tgttcagctt tgtggacctc cggctcctgc tcctcttagg
      361 ggccactgcc ctcctgacgc atggccaaga agatatcgtg agcaagggcg aggagctgtt
      421 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      481 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
      541 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct
      601 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      661 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      721 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      781 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      841 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
      901 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
      961 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
     1021 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1081 gatcactctc ggcatggacg agctgtacga gtcagaagat gtaggagtcg agggacccaa
     1141 gggagaccct ggcccccaag gcccaagggg acccgttggc ccccctggac gagatggcat
     1201 ccctggacag cctggacttc ctggtcctcc tggtccccct gggccccccg gaccccctgg
     1261 ccttggagga aactttgctt cccagatgtc ctatggctat gatgaaaaat cagctggagt
     1321 ttccgtgcct ggccccatgg gtccttctgg tcctcgtggt ctccctggcc cccctggtgc
     1381 acctggtcca caaggtttcc aaggcccccc tggtgaacct ggcgagcctg gcggttcagg
     1441 tccaatgggt ccccgaggtc cccctggccc tcctggcaag aatggagatg atggggaagc
     1501 tggcaagccc ggccgtcctg gtgagcgtgg acctcctgga cctcagggtg ctcgtggatt
     1561 gcctggaaca gctggcctcc ctggaatgaa gggacaccga ggcttcagtg gtttggatgg
     1621 tgccaaagga gatgctggtc ctgctggtcc taagggagag cccggcagtc ctggtgaaaa
     1681 cggagctcct ggccagatgg gtccccgagg tctgcccggt gagagaggtc gccctggacc
     1741 tcctggcact gctggtgctc gcggtaacga tggtgctgtt ggtgctgctg gaccccctgg
     1801 tcccaccggc cccactggcc ctcctggctt ccctggtgca gttggtgcta agggtgaagc
     1861 tggtccccaa ggagctagag gctctgaagg tccccagggt gtgcgtggtg agcccggacc
     1921 ccctggccct gctggtgctg ccggccctgc tggaaaccct ggtgctgatg gacaacctgg
     1981 cgctaaaggt gccaatggtg ctcctggtat tgctggtgct cctggcttcc ctggtgcccg
     2041 aggcccctct ggaccccagg gccccagcgg ccctccaggt cccaagggta acagtggtga
     2101 acctggtgct cctggcaaca aaggagacac tggtgccaaa ggagaacccg gtgctactgg
     2161 agttcaaggt cccccaggcc ctgccggaga agaaggaaaa cgaggagccc gtggtgagcc
     2221 tggaccttcc ggactgcctg gacctcctgg cgagcgtggt ggacctggta gccgtggttt
     2281 ccctggtgct gatggtgttg ctggccccaa gggtccttcc ggtgaacgtg gtgctcccgg
     2341 acctgctggt cccaaaggtt ctcctggtga agctggtcgc cccggtgaag ctggtctccc
     2401 tggtgccaag ggtctcactg gcagtcctgg cagccctggt cctgatggca aaaccggccc
     2461 ccctggtccc gctggtcaag atggtcgccc tggacccgca ggtcctcctg gagcccgtgg
     2521 ccaggctggt gtgatgggat tccctggacc taagggtacc gctggagaac ctggaaaggc
     2581 tggagagcga ggccttcccg gaccccctgg cgctgttggt cctgctggca aagatggaga
     2641 agctggagct cagggagccc ctggccctgc tggtcctgct ggtgagagag gtgaacaagg
     2701 tcccgctggc tcccctggat tccagggtct tcctggtcct gccggtcctc ctggtgaagc
     2761 aggcaagcct ggtgaacagg gtgttcctgg agaccttggt gcccctggac cctctggcgc
     2821 aagaggcgag agaggtttcc ctggtgaacg tggtgtacaa ggtcccccag gtcctgctgg
     2881 tccccgagga aacaatggtg cccccggcaa cgatggtgcc aagggtgata ctggtgcccc
     2941 cggagctccc ggtagccagg gtgcccccgg tcttcaggga atgcctggtg aacgtggtgc
     3001 agctggtctt ccaggtccta agggtgacag aggtgatgct ggtcccaaag gtgctgatgg
     3061 ttctcctggt aaagatggtg cccgtggtct gactggtccc attggtcctc ctggccctgc
     3121 tggtgcccct ggtgacaagg gtgaagctgg tcccagtggt cctcccggtc ccaccggagc
     3181 ccgtggtgct cccggagacc gtggtgaggc tggtccccct ggtcctgctg gctttgccgg
     3241 cccccctggt gctgatggcc aacctggtgc gaaaggtgaa cctggtgata ctggtgttaa
     3301 aggtgatgct ggtcctcctg gccctgctgg tcctgctgga ccccccggcc ccattggtaa
     3361 cgttggtgct cctggaccca aaggtcctcg tggtgctgct ggtccccctg gtgctactgg
     3421 cttccctggt gctgctggcc gtgtcggtcc ccctggtccc tctggaaatg ctggaccccc
     3481 tggccctccc ggtcccgttg gcaaagaagg gggcaaaggt ccccgtggtg agactggccc
     3541 tgctggacgt cctggtgaag ttggtccccc aggtcccccc ggtcctgctg gtgagaaagg
     3601 atctcctggt gctgatggac ctgctggctc tcctggtacc cctggacctc agggtattgc
     3661 tggacaacgt ggtgtggtcg gtcttcccgg tcagagagga gaaagaggct tccctggtct
     3721 tcctggcccc tctggtgaac ctggcaaaca aggtccttct ggatcaagtg gtgaacgcgg
     3781 tccccctggc cccatggggc cccctggatt ggctggtccc cctggtgaat ctggacgtga
     3841 gggatcccct ggtgctgaag gctcccctgg aagggatggt gctcccgggg ccaagggtga
     3901 ccgtggtgag actggccctg ctggcccccc tggtgcccct ggtgctcccg gtgctcccgg
     3961 ccctgttggt cccgctggca agaatggcga tcgtggtgag actggtcctg ctggtcctgc
     4021 tggtcccatt ggccctgctg gtgcccgtgg ccctgctgga ccccaaggcc cccgtggtga
     4081 caagggtgag acaggcgaac aaggtgacag aggcataaag ggtcatcgtg gcttctctgg
     4141 tctccagggt cctcctggtt ctcctggttc tcctggtgaa caaggcccct ctggagcttc
     4201 aggtcctgca ggcccccggg gtccccctgg ctctgctggt tctcctggca aagacggact
     4261 caacggtctc cctggcccca ttggtccccc tggtcctcga ggtcgcactg gtgacagcgg
     4321 ccctgctggt ccccccggcc ctcctggacc ccctggccct cctggacctc ccagtggcgg
     4381 ttatgacttc agcttcctgc ctcagccacc tcaagagaag tctcaagatg gtggccgcta
     4441 ctaccgggcc gatgatgcta acgtggttcg tgaccgtgac cttgaggtgg acaccaccct
     4501 caagagcctg agtcagcaga ttgagaacat ccgcagcccc gaaggcagcc gcaagaaccc
     4561 tgcccgcaca tgccgcgacc tcaagatgtg ccactctgac tggaagagcg gagagtactg
     4621 gatcgaccct aaccaaggct gcaacctgga cgccatcaag gtctactgca acatggagac
     4681 aggtcagacc tgtgtgttcc ctactcagcc gtctgtgcct cagaagaact ggtacatcag
     4741 cccgaacccc aaggaaaaga agcacgtctg gtttggagag agcatgaccg atggattccc
     4801 gttcgagtac ggaagcgagg gctccgaccc cgccgatgtc gctatccagc tgaccttcct
     4861 gcgcctaatg tccaccgagg cctcccagaa catcacctat cactgcaaga acagcgtagc
     4921 ctacatggac cagcagactg gcaacctcaa gaaggccctg ctcctccagg gatccaacga
     4981 gatcgagctc agaggcgaag gcaacagtcg cttcacctac agcacccttg tggacggctg
     5041 cacgagtcac accggaactt ggggcaagac agtcatcgaa tacaaaacca ccaagacctc
     5101 ccgcctgccc atcatcgatg tggctccctt ggacattggt gccccagacc aggaattcgg
     5161 actagacatt ggccctgcct gcttcgtgta aactccctcc accccaatct ggttccctcc
     5221 cacccagccc acttttcccc aaccctggaa acagacgaac aacccaaact caatttcccc
     5281 caaaagccaa aaatatggga gataatttca catggacttt ggaaaacatt ttttttcctt
     5341 tgcattcacc tttcaaactt agtttttacc tttgaccaac tgaacgtgac caaaaaccaa
     5401 aagtgcattc aaccttacca aaaaagaaaa aaaaaaaaaa agggcggccg ctcgagtcta
     5461 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     5521 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5581 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     5641 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     5701 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     5761 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5821 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5881 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5941 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6001 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6061 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6121 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6181 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     6241 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     6301 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6361 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6421 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     6481 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6541 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     6601 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     6661 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     6721 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     6781 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     6841 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     6901 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     6961 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     7021 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     7081 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     7141 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     7201 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     7261 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     7321 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     7381 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     7441 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     7501 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     7561 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7621 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7681 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     7741 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7801 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7861 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7921 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7981 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8041 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8101 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8161 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8221 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8281 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8341 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8401 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8461 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8521 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8581 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8641 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8701 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8761 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8821 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8881 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8941 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9001 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9061 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9121 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9181 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9241 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9301 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9361 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9421 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9481 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9541 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9601 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9661 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9721 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9781 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9841 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     9901 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     9961 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
    10021 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
    10081 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
    10141 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    10201 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
    10261 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
    10321 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
    10381 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
    10441 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
    10501 ctattaccat g
//
