LOCUS       sequence-233910-       10502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-1 chain (Col1a1) with 
            mApple replacing exon 2-3 retaining N-propeptide minor triple helix 
            and N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: mApple-pro-alpha-1(I)
FEATURES             Location/Qualifiers
     source          1..10502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             778..1479
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAF
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFE
                     DGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALKS
                     EIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAE
                     GRHSTGGMDELY"
     primer_bind     1188..1207
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5859..5876)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5865..6089
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6135..6563
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6222..6241)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6432..6453
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6572..6592)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6577..6906
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6757..6892
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6819..6838
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6973..7767
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7027..7046)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7637..7656
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7941..8062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7978..7997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8032..8051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8111..8127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8111..8127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8124..8146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8135..8151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8159..8165,8166..8183,8184..8189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8204..8225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8342..8359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8513..9101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8593..8612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9272..10063,10064..10132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9895..9914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10133..10237)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10312..10331)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttatgtctag ggtctagaca tgttcagctt tgtggacctc
      721 cggctcctgc tcctcttagg ggccactgcc ctcctgacgc atggccaaga agatatcgtg
      781 agcaagggcg aggagaataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      841 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      901 tacgaggcct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc
      961 tgggacatcc tgtcccctca gttcatgtac ggctccaagg tctacattaa gcacccagcc
     1021 gacatccccg actacttcaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg
     1081 aacttcgagg acggcggcat tattcacgtt aaccaggact cctccctgca ggacggcgtg
     1141 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     1201 aagaagacca tgggctggga ggcctccgag gagcggatgt accccgagga cggcgccctg
     1261 aagagcgaga tcaagaagag gctgaagctg aaggacggcg gccactacgc cgccgaggtc
     1321 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc
     1381 aagttggaca tcgtgtccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     1441 gagggccgcc actccaccgg cggcatggac gagctgtacg agtcagaaga tgtaggagtc
     1501 gagggaccca agggagaccc tggcccccaa ggcccaaggg gacccgttgg cccccctgga
     1561 cgagatggca tccctggaca gcctggactt cctggtcctc ctggtccccc tgggcccccc
     1621 ggaccccctg gccttggagg aaactttgct tcccagatgt cctatggcta tgatgaaaaa
     1681 tcagctggag tttccgtgcc tggccccatg ggtccttctg gtcctcgtgg tctccctggc
     1741 ccccctggtg cacctggtcc acaaggtttc caaggccccc ctggtgaacc tggcgagcct
     1801 ggcggttcag gtccaatggg tccccgaggt ccccctggcc ctcctggcaa gaatggagat
     1861 gatggggaag ctggcaagcc cggccgtcct ggtgagcgtg gacctcctgg acctcagggt
     1921 gctcgtggat tgcctggaac agctggcctc cctggaatga agggacaccg aggcttcagt
     1981 ggtttggatg gtgccaaagg agatgctggt cctgctggtc ctaagggaga gcccggcagt
     2041 cctggtgaaa acggagctcc tggccagatg ggtccccgag gtctgcccgg tgagagaggt
     2101 cgccctggac ctcctggcac tgctggtgct cgcggtaacg atggtgctgt tggtgctgct
     2161 ggaccccctg gtcccaccgg ccccactggc cctcctggct tccctggtgc agttggtgct
     2221 aagggtgaag ctggtcccca aggagctaga ggctctgaag gtccccaggg tgtgcgtggt
     2281 gagcccggac cccctggccc tgctggtgct gccggccctg ctggaaaccc tggtgctgat
     2341 ggacaacctg gcgctaaagg tgccaatggt gctcctggta ttgctggtgc tcctggcttc
     2401 cctggtgccc gaggcccctc tggaccccag ggccccagcg gccctccagg tcccaagggt
     2461 aacagtggtg aacctggtgc tcctggcaac aaaggagaca ctggtgccaa aggagaaccc
     2521 ggtgctactg gagttcaagg tcccccaggc cctgccggag aagaaggaaa acgaggagcc
     2581 cgtggtgagc ctggaccttc cggactgcct ggacctcctg gcgagcgtgg tggacctggt
     2641 agccgtggtt tccctggtgc tgatggtgtt gctggcccca agggtccttc cggtgaacgt
     2701 ggtgctcccg gacctgctgg tcccaaaggt tctcctggtg aagctggtcg ccccggtgaa
     2761 gctggtctcc ctggtgccaa gggtctcact ggcagtcctg gcagccctgg tcctgatggc
     2821 aaaaccggcc cccctggtcc cgctggtcaa gatggtcgcc ctggacccgc aggtcctcct
     2881 ggagcccgtg gccaggctgg tgtgatggga ttccctggac ctaagggtac cgctggagaa
     2941 cctggaaagg ctggagagcg aggccttccc ggaccccctg gcgctgttgg tcctgctggc
     3001 aaagatggag aagctggagc tcagggagcc cctggccctg ctggtcctgc tggtgagaga
     3061 ggtgaacaag gtcccgctgg ctcccctgga ttccagggtc ttcctggtcc tgccggtcct
     3121 cctggtgaag caggcaagcc tggtgaacag ggtgttcctg gagaccttgg tgcccctgga
     3181 ccctctggcg caagaggcga gagaggtttc cctggtgaac gtggtgtaca aggtccccca
     3241 ggtcctgctg gtccccgagg aaacaatggt gcccccggca acgatggtgc caagggtgat
     3301 actggtgccc ccggagctcc cggtagccag ggtgcccccg gtcttcaggg aatgcctggt
     3361 gaacgtggtg cagctggtct tccaggtcct aagggtgaca gaggtgatgc tggtcccaaa
     3421 ggtgctgatg gttctcctgg taaagatggt gcccgtggtc tgactggtcc cattggtcct
     3481 cctggccctg ctggtgcccc tggtgacaag ggtgaagctg gtcccagtgg tcctcccggt
     3541 cccaccggag cccgtggtgc tcccggagac cgtggtgagg ctggtccccc tggtcctgct
     3601 ggctttgccg gcccccctgg tgctgatggc caacctggtg cgaaaggtga acctggtgat
     3661 actggtgtta aaggtgatgc tggtcctcct ggccctgctg gtcctgctgg accccccggc
     3721 cccattggta acgttggtgc tcctggaccc aaaggtcctc gtggtgctgc tggtccccct
     3781 ggtgctactg gcttccctgg tgctgctggc cgtgtcggtc cccctggtcc ctctggaaat
     3841 gctggacccc ctggccctcc cggtcccgtt ggcaaagaag ggggcaaagg tccccgtggt
     3901 gagactggcc ctgctggacg tcctggtgaa gttggtcccc caggtccccc cggtcctgct
     3961 ggtgagaaag gatctcctgg tgctgatgga cctgctggct ctcctggtac ccctggacct
     4021 cagggtattg ctggacaacg tggtgtggtc ggtcttcccg gtcagagagg agaaagaggc
     4081 ttccctggtc ttcctggccc ctctggtgaa cctggcaaac aaggtccttc tggatcaagt
     4141 ggtgaacgcg gtccccctgg ccccatgggg ccccctggat tggctggtcc ccctggtgaa
     4201 tctggacgtg agggatcccc tggtgctgaa ggctcccctg gaagggatgg tgctcccggg
     4261 gccaagggtg accgtggtga gactggccct gctggccccc ctggtgcccc tggtgctccc
     4321 ggtgctcccg gccctgttgg tcccgctggc aagaatggcg atcgtggtga gactggtcct
     4381 gctggtcctg ctggtcccat tggccctgct ggtgcccgtg gccctgctgg accccaaggc
     4441 ccccgtggtg acaagggtga gacaggcgaa caaggtgaca gaggcataaa gggtcatcgt
     4501 ggcttctctg gtctccaggg tcctcctggt tctcctggtt ctcctggtga acaaggcccc
     4561 tctggagctt caggtcctgc aggcccccgg ggtccccctg gctctgctgg ttctcctggc
     4621 aaagacggac tcaacggtct ccctggcccc attggtcccc ctggtcctcg aggtcgcact
     4681 ggtgacagcg gccctgctgg tccccccggc cctcctggac cccctggccc tcctggacct
     4741 cccagtggcg gttatgactt cagcttcctg cctcagccac ctcaagagaa gtctcaagat
     4801 ggtggccgct actaccgggc cgatgatgct aacgtggttc gtgaccgtga ccttgaggtg
     4861 gacaccaccc tcaagagcct gagtcagcag attgagaaca tccgcagccc cgaaggcagc
     4921 cgcaagaacc ctgcccgcac atgccgcgac ctcaagatgt gccactctga ctggaagagc
     4981 ggagagtact ggatcgaccc taaccaaggc tgcaacctgg acgccatcaa ggtctactgc
     5041 aacatggaga caggtcagac ctgtgtgttc cctactcagc cgtctgtgcc tcagaagaac
     5101 tggtacatca gcccgaaccc caaggaaaag aagcacgtct ggtttggaga gagcatgacc
     5161 gatggattcc cgttcgagta cggaagcgag ggctccgacc ccgccgatgt cgctatccag
     5221 ctgaccttcc tgcgcctaat gtccaccgag gcctcccaga acatcaccta tcactgcaag
     5281 aacagcgtag cctacatgga ccagcagact ggcaacctca agaaggccct gctcctccag
     5341 ggatccaacg agatcgagct cagaggcgaa ggcaacagtc gcttcaccta cagcaccctt
     5401 gtggacggct gcacgagtca caccggaact tggggcaaga cagtcatcga atacaaaacc
     5461 accaagacct cccgcctgcc catcatcgat gtggctccct tggacattgg tgccccagac
     5521 caggaattcg gactagacat tggccctgcc tgcttcgtgt aaactccctc caccccaatc
     5581 tggttccctc ccacccagcc cacttttccc caaccctgga aacagacgaa caacccaaac
     5641 tcaatttccc ccaaaagcca aaaatatggg agataatttc acatggactt tggaaaacat
     5701 tttttttcct ttgcattcac ctttcaaact tagtttttac ctttgaccaa ctgaacgtga
     5761 ccaaaaacca aaagtgcatt caaccttacc aaaaaagaaa aaaaaaaaaa aagggcggcc
     5821 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     5881 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     5941 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6001 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     6061 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     6121 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6181 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6241 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6301 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     6361 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     6421 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     6481 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     6541 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     6601 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6661 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6721 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6781 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6841 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     6901 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     6961 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     7021 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     7081 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     7141 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     7201 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     7261 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     7321 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     7381 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     7441 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     7501 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     7561 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     7621 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     7681 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     7741 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     7801 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     7861 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     7921 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     7981 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8041 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     8101 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8161 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8221 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8281 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8341 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8401 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8461 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8521 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8581 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8641 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8701 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8761 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8821 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8881 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8941 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9001 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9061 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9121 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9181 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9241 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9301 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9361 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9421 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9481 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9541 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9601 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9661 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9721 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9781 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9841 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9901 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9961 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10021 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10081 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10141 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10201 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10261 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
    10321 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
    10381 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
    10441 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
    10501 tt
//
