LOCUS       sequence-233911-       10304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-2 chain (Col1a2) with 
            mApple replacing exon 2-3 retaining N-propeptide minor triple helix 
            and N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10304)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: mApple-pro-alpha-2(I)-L
FEATURES             Location/Qualifiers
     source          1..10304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             805..1509
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAF
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFE
                     DGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALKS
                     EIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     1215..1234
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5661..5678)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5667..5891
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5937..6365
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6024..6043)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6234..6255
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6374..6394)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6379..6708
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6559..6694
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6621..6640
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6775..7569
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6829..6848)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7439..7458
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7743..7864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7780..7799)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7834..7853
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7913..7929)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7913..7929)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7926..7948)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7937..7953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7961..7967,7968..7985,7986..7991))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8006..8027
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8144..8161)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8315..8903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8395..8414)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9074..9865,9866..9934))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9697..9716
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9935..10039)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10114..10133)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttcgtagaca tgctcagctt tgtggatacg cggactctgt tgctgcttgc agtaacttcg
      781 tgcctagcaa catgccaaga tatcgtgagc aagggcgagg agaataacat ggccatcatc
      841 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      901 atcgagggcg agggcgaggg ccgcccctac gaggcctttc agaccgctaa gctgaaggtg
      961 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     1021 tccaaggtct acattaagca cccagccgac atccccgact acttcaagct gtccttcccc
     1081 gagggcttca ggtgggagcg cgtgatgaac ttcgaggacg gcggcattat tcacgttaac
     1141 caggactcct ccctgcagga cggcgtgttc atctacaagg tgaagctgcg cggcaccaac
     1201 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccgaggag
     1261 cggatgtacc ccgaggacgg cgccctgaag agcgagatca agaagaggct gaagctgaag
     1321 gacggcggcc actacgccgc cgaggtcaag accacctaca aggccaagaa gcccgtgcag
     1381 ctgcccggcg cctacatcgt cgacatcaag ttggacatcg tgtcccacaa cgaggactac
     1441 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     1501 ctgtacaagg gccccactgg agacagagga ccacgtggac aaaggggtcc cgcaggtccc
     1561 cgaggcagag atggtgttga tggtcccatg ggccctcctg gtccccctgg ctcccctggt
     1621 cctcccggct cccctgcccc ccctggtctt actgggaact ttgctgctca gtattctgac
     1681 aaaggagttt catctggccc tggaccaatg ggtttaatgg gacccagagg ccctcctggt
     1741 gctgttggag cccctggccc tcaaggtttc caaggacctg ctggtgaacc tggtgagcct
     1801 ggtcaaacgg gtcctgcagg tccccgtggt ccagctggct ctcctggcaa ggctggtgag
     1861 gatggtcacc ctggaaaacc cggaagacct ggggagagag gagtcgttgg accacagggt
     1921 gctcgtggtt tccccgggac tcctggactt cctggcttca aaggcgtgaa aggacacagt
     1981 ggtatggatg gattaaaggg acagcctggt gcacagggtg tgaagggtga acccggtgcc
     2041 cctggagaga atggaactcc aggtcaagca ggagcccgag ggcttcctgg tgagagaggg
     2101 cgtgtcggag ctcctggtcc agctggtgcc cgaggcagtg atggaagcgt tggccccgtc
     2161 ggccctgctg gtcccattgg gtctgctgga ccccctggtt tcccaggtgc tcctggtccc
     2221 aagggtgaac ttgggcccgt tggtaaccct ggccctgctg gtcctgctgg tccccgtggt
     2281 gaagtgggtc ttccaggtct ttctggccct gttggacctc ctggcaaccc tggaacaaat
     2341 gggctcactg gcgccaaggg tgctactgga ctccctggtg tcgctggggc tcccggtctc
     2401 cctggccccc gtggtattcc tggccccgct ggtgctgctg gtgctacagg tgccagagga
     2461 cttgttggtg agcctggtcc tgctggctcc aaaggagaat ccggtaacaa gggtgagcct
     2521 ggctctgttg gagcccaagg tcctcctggt cccagcggtg aagaaggaaa gagagggtct
     2581 cccggagaag ctggatctgc tggccctgca gggcctccag ggcttagagg cagccctggt
     2641 tctcgaggtc ttcctggagc tgatggcaga gctggtgtaa tgggtcctcc tggcaatcgt
     2701 ggttcaaccg gccctgctgg aatccgaggt cctaatggag atgccggtcg acctggggaa
     2761 cctggtctca tgggacccag aggtcttcct gggtctcctg gaaatgttgg cccatctggt
     2821 aaagaaggcc ctgtgggtct ccctggcatt gatggcagac ctggcccaat cggcccagct
     2881 ggaccaagag gtgaagctgg caacattgga ttccctggac ccaaaggccc ctctggtgat
     2941 cctggcaaac ctggtgagag aggacacccc ggtcttgctg gtgctcgggg agctccagga
     3001 cccgatggca acaatggagc tcagggcccc cctggacccc agggtgttca aggtggcaaa
     3061 ggtgaacaag gccctgctgg tcctcctggc ttccagggtc tccctggtcc ctcaggtact
     3121 actggagaag ttggcaagcc cggagaaagg ggtcttcctg gtgaattcgg tctccctggt
     3181 cctgctggtc caagaggaga acgtggtacc ccgggtgaga gtggagctgc tggcccttct
     3241 ggtcctattg gaagccgagg tcccagtgga gccccagggc ctgatggaaa caagggtgaa
     3301 gctggtgcag tcggtgctcc aggcagtgct ggtgcctctg ggcctggtgg gcttccagga
     3361 gagaggggtg ctgctggcat acctgggggc aaaggagaaa agggtgaaac tggtctccga
     3421 ggtgacactg gcaacactgg tagagatggt gctcgtggca ttcctggtgc tgtaggtgcc
     3481 cctggtcctg ctggggcctc aggtgaccgg ggtgaagctg gtgctgccgg tccttctggc
     3541 ccagctggtc ctcggggtag ccctggtgaa cgtggtgaag ttggccctgc tggccccaat
     3601 ggatttgctg gtcctgctgg tgctgctggc caacccggtg ctaaaggaga gaagggaacc
     3661 aaagggccta agggtgaaaa tggcattgtt ggtccaaccg ggtctgttgg agctgctggc
     3721 ccatctggtc ctaatggccc acctggtcct gttggaagtc gtggtgatgg agggccccct
     3781 ggtatgactg gcttccctgg tgctgctgga cggactggtc cccccggacc cagtggtatt
     3841 gctggccccc ctggcccccc tggtgctgct ggaaaggaag ggattcgtgg tccccgtggc
     3901 gaccaaggtc cagttggccg aactggagaa acaggtgcat ctggaccccc tggatttgtt
     3961 ggtgaaaagg gtccctctgg agaacctggt accgcaggag cccctggtac cgcaggtcct
     4021 cagggtcttc ttggtgctcc cggtattctg ggtctccctg gctctagagg tgaacgtggt
     4081 ctgccaggta ttgctggtgc tctgggtgaa cctggtcctc tgggcatctc aggccctcct
     4141 ggtgcccgtg gtccccctgg tgctgtgggc agccctggag ttaatggtgc ccctggtgaa
     4201 gctggtcgtg atggtaaccc tggcagcgat ggtcccccag gtcgtgatgg tcagcctgga
     4261 cacaagggag agcgtggtta ccctggcagc attggtccga ctggtgctgc aggtgcccct
     4321 ggtcctcacg gttctgtggg tcctgctggg aaacatggaa accgaggtga acctggtcct
     4381 gccggttctg ttggtcctgt tggtgctgtt ggtccaagag gtcctagtgg tcctcaaggc
     4441 atccgaggtg acaaaggaga acctggtgat aaagggcaca ggggtcttcc tggcttaaaa
     4501 ggatacagtg gattgcaggg tcttcctggt cttgctggcc tacatggtga ccaaggagct
     4561 cctggtcctg tgggtcctgc tggtcccagg ggtcctgctg ggccttctgg acccgttggc
     4621 aaagatggta gatctgggca acctggccca gtcgggcctg ctggtgttcg tggttctcag
     4681 ggtagccaag gtcccgctgg tccccctggt ccccctggac ctcctggtcc tcctggtgtc
     4741 agcggaggtg gctatgactt tggttttgaa ggagacttct acagggctga ccagcctcgc
     4801 tcccagcctt cactcagacc caaggactat gaagttgatg caactctgaa atctctcaat
     4861 aaccagattg agacccttct cactcctgaa ggctctagaa agaaccctgc tcgcacgtgc
     4921 cgggacttaa gactcagcca cccagagtgg aacagcgatt actactggat tgaccctaac
     4981 caaggatgta ctatggatgc catcaaagtg tactgtgatt tctctactgg tgaaacctgc
     5041 atccaggccc aacctgtaaa caccccagcg aagaactcat acagccgcgc ccaggccaac
     5101 aagcatgtct ggttaggaga gactatcaat ggtggcagcc agtttgaata caacgtagaa
     5161 ggggtgtcct ccaaggaaat ggcaactcag ctcgccttca tgcgcctgct agccaaccgt
     5221 gcttctcaga acatcaccta ccactgcaag aacagcattg cgtacctgga tgaggagacg
     5281 ggcagcttga acaaggctgt gcttctgcag ggttccaacg atgttgaact tgttgctgag
     5341 ggcaacagca ggttcaccta ctctgtccta gtcgatggct gctccaaaaa gacaaatgaa
     5401 tggggcaaga caatcattga atacaaaaca aataagccat ctcgcctgcc gttccttgac
     5461 attgcacctc tggacattgg tggtgctgac caagaattcc gtgtggaggt tggccccgtc
     5521 tgtttcaaat aagtgaactc aacataaatt aaaaaccaaa aacccctgaa aaaactttct
     5581 ctttgccgtt tcctccttta aaaaaaaaaa aaaagggcgg ccgctcgagt ctagagggcc
     5641 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5701 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5761 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5821 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     5881 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     5941 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     6001 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     6061 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     6121 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     6181 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     6241 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     6301 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6361 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     6421 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     6481 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     6541 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6601 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     6661 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     6721 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     6781 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     6841 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     6901 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     6961 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     7021 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     7081 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     7141 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     7201 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     7261 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     7321 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     7381 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     7441 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     7501 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     7561 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     7621 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     7681 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     7741 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7801 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7861 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7921 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7981 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8041 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8101 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8161 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8221 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8281 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8341 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8401 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8461 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8521 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8581 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8641 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8701 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8761 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8821 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8881 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8941 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9001 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9061 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9121 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9181 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9241 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9301 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9361 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9421 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9481 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9541 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9601 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9661 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9721 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9781 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9841 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9901 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9961 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10021 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    10081 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    10141 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
    10201 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
    10261 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
