LOCUS       sequence-233913-       10184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of MBP-Pumillio1 fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Tichon A, Gil N, Lubelsky Y, Havkin Solomon T, Lemze D, Itzkovitz S,
            Stern-Ginossar N, Ulitsky I
  TITLE     A conserved abundant cytoplasmic long noncoding RNA modulates 
            repression by Pumilio proteins in human cells.
  JOURNAL   Nat Commun. 2016 Jul 13;7:12209. doi: 10.1038/ncomms12209.
  PUBMED    27406171
REFERENCE   2  (bases 1 to 10184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-13"; volume: "7:12209. doi"; pages: " 
            10.1038/ncomms12209"
COMMENT     Sequence Label: pMAL-PUM1
FEATURES             Location/Qualifiers
     source          1..10184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..115
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             116..1198
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(135..154)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1441..1446,1447..1462,1463..1469)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1477..1493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1482..1504
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1563..2663
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFLAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGSYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      2520..2529
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2637..2660
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2676..2693
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2715..2735
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(6312..6329)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6312..6328)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6321..6343)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6507..6524)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6677..6763
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6855..6882
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        6901..6992
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6993..7061,7062..7853)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7211..7230)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7895..8408)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8090..8111)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8302..8321
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      8519..9107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9008..9027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9261..9278
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9293..9433
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9359..9378)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9519..9541
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9535..9726)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gcacccgtgg ccaggaccca acgctgcccg aaattccgac accatcgaat ggtgcaaaac
       61 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa
      121 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
      181 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat
      241 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt
      301 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
      361 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
      421 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
      481 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa
      541 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc
      601 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat
      661 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca
      721 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc
      781 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
      841 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
      901 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta
      961 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
     1021 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
     1081 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
     1141 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     1201 gcaacgcaat taatgtaagt tagctcactc attaggcaca attctcatgt ttgacagctt
     1261 atcatcgact gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt
     1321 atggctgtgc aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc actcccgttc
     1381 tggataatgt tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg
     1441 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca
     1501 caggaaacag ccagtccgtt taggtgtttt cacgagcact tcaccaacaa ggaccataga
     1561 ttatgaaaac tgaagaaggt aaactggtaa tctggattaa cggcgataaa ggctataacg
     1621 gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc accgttgagc
     1681 atccggataa actggaagag aaattcccac aggttgcggc aactggcgat ggccctgaca
     1741 ttatcttctt ggcacacgac cgctttggtg gctacgctca atctggcctg ttggctgaaa
     1801 tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat gccgtacgtt
     1861 acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg atttataaca
     1921 aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg gataaagaac
     1981 tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc
     2041 cgctgattgc tgctgacggg agttatgcgt tcaagtatga aaacggcaag tacgacatta
     2101 aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg gttgacctga
     2161 ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct gcctttaata
     2221 aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc gacaccagca
     2281 aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg
     2341 ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg gcaaaagagt
     2401 tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa gacaaaccgc
     2461 tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca cgtattgccg
     2521 ccaccatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt
     2581 tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag actgtcgatg
     2641 aagccctgaa agacgcgcag actaattcga gctcacatca tcatcatcat catgattatg
     2701 atatcccaac caccgaaaat ttgtattttc aagggatgag cgttgcatgt gtcttgaaga
     2761 gaaaagcagt gctttggcag gactctttca gcccccacct gaaacatcac cctcaagaac
     2821 cagctaatcc caacatgcct gttgttttga catctggaac agggtcgcaa gcgcagccac
     2881 aaccagctgc aaatcaggct cttgcagctg ggactcactc cagccctgtc ccaggatcta
     2941 taggagttgc aggccgttcc caggacgacg ctatggtgga ctacttcttt cagaggcagc
     3001 atggtgagca gcttggggga ggaggaagtg gaggaggcgg ctataataat agcaaacatc
     3061 gatggcctac tggggataac attcatgcag aacatcaggt gcgttccatg gatgaactga
     3121 atcatgattt tcaagcactt gctctggagg gaagagcgat gggagagcag ctcttgccag
     3181 gtaaaaagtt ttgggaaaca gatgaatcca gcaaagatgg accaaaagga atattcctgg
     3241 gtgatcaatg gcgagacagt gcctggggaa catcagatca ttcagtttcc cagccaatca
     3301 tggtgcagag aagacctggt cagagtttcc atgtgaacag tgaggtcaat tctgtactgt
     3361 ccccacgatc ggagagtggg ggactaggcg ttagcatggt ggagtatgtg ttgagctcat
     3421 ccccgggcga ttcctgtcta agaaaaggag gatttggccc aagggatgca gacagtgatg
     3481 aaaacgacaa aggtgaaaag aagaacaagg gtacgtttga tggagataag ctaggagatt
     3541 tgaaggagga gggtgatgtg atggacaaga ccaatggttt accagtgcag aatgggattg
     3601 atgcagacgt caaagatttt agccgtaccc ctggtaattg ccagaactct gctaatgaag
     3661 tggatcttct gggtccaaac cagaatggtt ctgagggctt agcccagctg accagcacca
     3721 atggtgccaa gcctgtggag gatttctcca acatggagtc ccagagtgtc cccttggacc
     3781 ccatggaaca tgtgggcatg gagcctcttc agtttgatta ttcaggcacg caggtacctg
     3841 tggactcagc agcagcaact gtgggacttt ttgactacaa ttctcaacaa cagctgttcc
     3901 aaagacctaa tgcgcttgct gtccagcagt tgacagctgc tcagcagcag cagtatgcac
     3961 tggcagctgc tcatcagccg cacatcggtt tagctcccgc tgcgtttgtc cccaatccat
     4021 acatcatcag cgctgctccc ccagggacgg acccctacac agctggattg gctgcagcag
     4081 cgacactagg cccagctgtg gtccctcacc agtattatgg agttactccc tggggagtct
     4141 accctgccag tcttttccag cagcaagctg ccgctgccgc tgcagcaact aattcagcta
     4201 atcaacagac caccccacag gctcagcaag gacagcagca ggttctccgt ggaggagcca
     4261 gccaacgtcc tttgacccca aaccagaacc agcagggaca gcaaacggat ccccttgtgg
     4321 cagctgcagc agtgaattct gcccttgcat ttggacaagg tctggcagca ggcatgccag
     4381 gttatccggt gttggctcct gctgcttact atgaccaaac tggtgccctt gtagtgaatg
     4441 caggcgcgag aaatggtctt ggagctcctg ttcgacttgt agctcctgcc ccagtcatca
     4501 ttagttcctc agctgcacaa gcagctgttg cagcagccgc agcttcagca aatggagcag
     4561 ctggtggtct tgctggaaca acaaatggac catttcgccc tttaggaaca cagcagcctc
     4621 agccccagcc ccagcagcag cccaataaca acctggcatc cagttctttc tacggcaaca
     4681 actctctgaa cagcaattca cagagcagct ccctcttctc ccagggctct gcccagcctg
     4741 ccaacacatc cttgggattc ggaagtagca gttctctcgg cgccaccctg ggatccgccc
     4801 ttggagggtt tggaacagca gttgcaaact ccaacactgg cagtggctcc cgccgtgact
     4861 ccctgactgg cagcagtgac ctttataaga ggacatcgag cagcttgacc cccattggac
     4921 acagttttta taacggcctt agcttttcct cctctcctgg acccgtgggc atgcctctcc
     4981 ctagtcaggg accaggacat tcacagacac cacctccttc cctctcttca catggatcct
     5041 cttcaagctt aaacctggga ggactcacga acggcagtgg aagatacatc tctgctgctc
     5101 caggcgctga agccaagtac cgcagtgcaa gcagcgcctc cagcctcttc agcccgagca
     5161 gcactctttt ctcttcctct cgtttgcgat atggaatgtc tgatgtcatg ccttctggca
     5221 ggagcaggct tttggaagat tttcgaaaca accggtaccc caatttacaa ctgcgggaga
     5281 ttgctggaca tataatggaa ttttcccaag accagcatgg gtccagattc attcagctga
     5341 aactggagcg tgccacacca gctgagcgcc agcttgtctt caatgaaatc ctccaggctg
     5401 cctaccaact catggtggat gtgtttggta attacgtcat tcagaagttc tttgaatttg
     5461 gcagtcttga acagaagctg gctttggcag aacggattcg aggccacgtc ctgtcattgg
     5521 cactacagat gtatggctgc cgtgttatcc agaaagctct tgagtttatt ccttcagacc
     5581 agcaggtaat taatgagatg gttcgggaac tagatggcca tgtcttgaag tgtgtgaaag
     5641 atcagaatgg caatcacgtg gttcagaaat gcattgaatg tgtacagccc cagtctttgc
     5701 aatttatcat cgatgcgttt aagggacagg tatttgcctt atccacacat ccttatggct
     5761 gccgagtgat tcagagaatc ctggagcact gtctccctga ccagacactc cctattttag
     5821 aggagcttca ccagcacaca gagcagcttg tacaggatca atatggaaat tatgtaatcc
     5881 aacatgtact ggagcacggt cgtcctgagg ataaaagcaa aattgtagca gaaatccgag
     5941 gcaatgtact tgtattgagt cagcacaaat ttgcaagcaa tgttgtggag aagtgtgtta
     6001 ctcacgcctc acgtacggag cgcgctgtgc tcatcgatga ggtgtgcacc atgaacgacg
     6061 gtccccacag tgccttatac accatgatga aggaccagta tgccaactac gtggtccaga
     6121 agatgattga cgtggcggag ccaggccagc ggaagatcgt catgcataag atccggcccc
     6181 acatcgcaac tcttcgtaag tacacctatg gcaagcacat tctggccaag ctggagaagt
     6241 actacatgaa gaacggtgtt gacttagggc ccatctgtgg cccccctaat ggtatcatct
     6301 gaaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6361 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6421 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggca gcttggctgt
     6481 tttggcggat gagataagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     6541 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     6601 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     6661 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     6721 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     6781 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     6841 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
     6901 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6961 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7021 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7081 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7141 acagcggtaa gatccttgag agttttcgcc ccgaagaacg tttcccaatg atgagcactt
     7201 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     7261 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7321 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7381 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7441 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7501 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7561 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7621 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7681 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7741 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7801 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7861 accaagttta ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc
     7921 caaaaacagg aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa
     7981 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     8041 aatcccttat aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa
     8101 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     8161 gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg
     8221 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     8281 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     8341 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     8401 gggcgcgtaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8461 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8521 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8581 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8641 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     8701 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8761 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8821 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8881 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8941 gaaaggcgga caggtatccg gtaagcggca gggtcgaaac aggagagcgc acgagggagc
     9001 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9061 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9121 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9181 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9241 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     9301 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     9361 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     9421 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     9481 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     9541 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     9601 gtgcagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     9661 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     9721 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     9781 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     9841 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     9901 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     9961 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
    10021 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
    10081 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
    10141 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgc
//
