LOCUS       sequence-233927-       10477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc4::QF2::QS::mKate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Mao S, Qi Y, Zhu H, Huang X, Zou Y, Chi T
  TITLE     A Tet/Q Hybrid System for Robust and Versatile Control of Transgene 
            Expression in C. elegans.
  JOURNAL   iScience. 2019 Jan 25;11:224-237. doi: 10.1016/j.isci.2018.12.023. 
            Epub 2018 Dec 27.
  PUBMED    30634168
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-01-25"; volume: "11:224-237. doi"; pages: " 
            10.1016/j.isci.2018.12.023. Epub 2018 Dec 27"
COMMENT     Sequence Label: TC690
FEATURES             Location/Qualifiers
     source          1..10477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3718..3774
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6544..6600
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(8355..8377)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8477..8496
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8574..8592)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8660..8764
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8765..8833,8834..9625)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8983..9002)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9796..10384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10285..10304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaccgtatta ccgcggccgc caacaatata ggatgctcag gagactataa ttcaaaaaac
       61 ccaacccaaa ttagcataag cttccccaaa ttggaacagt gaaatactca atcaatcact
      121 ctggcggttt tctccacaaa gaacacgaaa ttggaaaaag gaaaagattg atttgtttga
      181 aggcgtgagg aaatcgaaaa aaaattgttt caaaacaaat gaaatatttt aacgaataaa
      241 cccaggaatg ccactttttg gtgcccgcct acaaaaaagt caactgttct taaactccta
      301 catagttgcc ctctctaacg aatgttgctt tttttaaatt ttcattgaat ttgattatcg
      361 tctgtatgcg cctactctat cagtggaaaa catgcccatt tcccacaaat ttactcgttt
      421 ggagctcaaa atgttaaatg gggcatcagg gaattggcat ctcacagaaa gtggcggatg
      481 agttatatgt aataataatg gtggctgaag tggaaaccgc aaaaaaagga tgtgtatttg
      541 agattttgca acggaatttg aatcagatac acaaaatgag agaaatttat agattcttta
      601 aaatccacct gaagtcggca aatttttgaa aggtctgttc gttcagtttt tcataagttt
      661 tatgaatttt gatttactac tgaagttcga gaaaagccct ttaaccatac caataagaaa
      721 ttatgttaac tttacaagaa tattaacaaa aaattattaa aaaactgtgt tcccctggca
      781 ttatgatatt tccaccgtca gttctctatt aaaacttcaa ctctacacag tttcaactct
      841 caaatgagct gtccatcttt tgccttcagg aaattatgct aatctgctga tttcgcgcac
      901 tttcaccaca ctatttccaa atagaaggag gggtggtgca acacacgaga cactgcaaaa
      961 aggttcagtg aagatttatc tacttgtgag atgagagcag gaggggtggg agaagtgtga
     1021 tgattgacgg gatgacaaga acagtgatgt ggtgtcgaaa tggaagatgc gctaatttgt
     1081 ggagttttat gaaatttgaa taattacatt tataacatct actagttagg cgtctgtgta
     1141 ggtattgcct gcctgcctac aaggcatatg tacgaggcag gttaaacctc ctagaaagct
     1201 acggtacttt caaaccacat aacatttata ctggtgtctt ttcgcgtctt cttcttgaac
     1261 atgtagaccc tcaaaaagct aagttttaca ctgtgtagtt tgctttgaaa tctacaaaaa
     1321 ttggaggcac gccttctgga ctttgccgac acgcctactt tctacctacc aactgcctac
     1381 ttccacagtc tagcgtgaac tccaatacca ttttaaaaat tgttttatct caaattttca
     1441 atttttaaat ccaatttccc agttctaaaa aaaaaatttt ttttttaatt taaaaactct
     1501 catccttttt aatgctcgtt cccattcggc aagacgtgta ccaaacacaa aacaaattga
     1561 ctggtgatcc atctcgaatt tctcgtttca aattagtcgt tgctatatat attgtcttat
     1621 gtgtggaaaa cgaataagaa aaattagaga aagatgagag agcaggggca gaatgaaaat
     1681 ttgcatggaa attgaagaag atcactgctt tttccgatga atgatgacta aatagaatga
     1741 gttgagagga aaataatcat aattctaatt ttaattagaa atatgatatg gaaagtaagc
     1801 aaaaccacaa ggcttttgct ctgaacttat ttccgaaaaa aaaagtgaaa ctgaaagtta
     1861 gaattttcaa aataaatatt gcgagagtca accttcagaa aaattggcga gaaatcaaat
     1921 ttgaattctc tgaaaattta gttaaaatac atatgttatc cttctttgtt ttttggcctt
     1981 agcctgcctt caaggcgacc tgagcctatg cgggtgccta ataccgaaac acacataatt
     2041 ttttttttta atctttaaat actgtaacga gtcgcgagat ccagcgagac ccatagaata
     2101 tgttgagcgc tgcaaacaat cgtataactc ttgtccaaat ttttccactc tgtatttttg
     2161 caagcagttc aacaaatggt catcattttt ataacccata tctatattcg aatctgacat
     2221 aacaaataat actaagcaca tcctcccaca tctcttttca ttcaagttgc gcaattcccc
     2281 taataactat ggatggaggg gggaccgagc ccaaagatta ttcaaaacga taacgacttt
     2341 ttcatgcggc caacgaaaaa taaaactttt ctcatattct cctatttccc atttcaagaa
     2401 ttacatcccc atgggacgtt gaaatcacat catattagta tatgttaatt gttcttttca
     2461 ctggaatatc gccacgcccc ccttccggtg gctcattggt ttcgagaaga aggcggagcc
     2521 gaagtgggtt cttctttctt ttttttgtgg tagaagtcta gcaggattaa gctactattc
     2581 cattagagga tcttcttctt ccaaaaagtg aaaatgccgc ctaaacgcaa gacactcaat
     2641 gccgctgccg aagccaatgc ccacgctgat ggccatgctg atggcaatgc tgatggtcat
     2701 gtcgctaaca ctgcagcaag cagcaacaac gcccgttttg cggacttgac caacattgac
     2761 acacccggcc tcggccctac cacgacgacg ttacttgtcg agcccgctcg ttcgaaacgc
     2821 cagagagtct cgagggcctg tgatcagtgt cgagctgcac gtgaaaagtg tgatggaatc
     2881 cagccggctt gcttcccctg tgtgtcgcag ggccggtcgt gtacctacca ggccagtccc
     2941 aagaagcgag gagtccagac gggctacatc cgcactctcg aactggctct ggcttggatg
     3001 ttcgagaacg ttgcccgcag cgaggacgcc ctccacaatc ttttggtccg tgatgctggc
     3061 cagggcagcg ctctcctggt cggcaaagac tcgcctgctg cagaacgcct gcatgcaaga
     3121 tgggcgacga gtcgagtcaa caaaagcatc acccgtcttc tctcaggtgg atctggatct
     3181 ggatctggat ctggttccgg tcgacaactt gagcttccgc cgactgcgac tgccactgca
     3241 agtatcatgc cccatgtcat ggaacagccc ctgtcaacgt caataaatcc ggtcaatgat
     3301 cggtttaatg gaataccgaa tcctaccccc tacaatagcg atgcggccct agacgcaatt
     3361 actcaaacca atgactacgg atcagtcaat acccatggca ttctcagtac atatccacca
     3421 cctgccaccc acttgaacga ggcctcggtg gcccttgcgc ctggaggtgc tcctcccaga
     3481 ccacccccgc catacgttga tagtacaacg aaccatcctc cttaccatag taacctcgtc
     3541 cccatggcca atttcggata ttcgactgta gactatgatg cgatggtgga tgatctggca
     3601 tcgatcgagt acacggatgc ggttgacgtt gatccgcagt tcatgacgaa cctcgggttt
     3661 gtcccagggt gtaattttag cgatatcaac acgtatgagc aaccatgggg cagtggggct
     3721 actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctatgaac
     3781 accatcccgg cacgccatgt cggggatgtc gccgcccgcg atcctctacc tctaccgcac
     3841 atatcatcct ccgtcgccag cggcatgaag cgttccttcg caaccatggc catgctctac
     3901 aacgacactg gcaacagcaa cgatgtcggt gcccatgcca ggcgaccacc acgaaccctc
     3961 tccaatagtc ggagcacttc cgcccacaga gtacctctag gctcttggtc ggcgcccaat
     4021 tccccgcccc gccgtgcgct gccgcatcat cccatcaccg ccagcttcga tcccgatgct
     4081 tccattgtta tcgccggcat tcgtggcgct ggcaagtcta cgctggccat catggcatct
     4141 accgccatga agcgcaagat agtcgacctg gaatccgagt tccatcatct tactggcttg
     4201 tctagttcca gctacaagaa gacacacggc ccggtcgact atgggaggcg ccagatcgcc
     4261 atcttgcaga acatcttgaa tctgcacagg acccgcgcca ttctcgtctg ctcttggctg
     4321 gagcgggatg tgcaggccat gttgcaggat ttcagcgtgt ctaatcctgt catttacgtt
     4381 ctgcgcgatg ccaaagctat cgaggcccat ctgaagggat acgacaagtc caaagttggc
     4441 accctccttg atgccaccag tactgtcctt cgccgctgca cccgtttcga gttcttcaac
     4501 gtctccgaag agaacctgga cacccactcc gcttcaacat caccacctgc tgttccggac
     4561 cagcggcata ccgcgccgta tctaacgctt aaacgagccg agcgccactt cctcaaattt
     4621 ctctccttga ttctacccaa ggggaccata ccttttgtcg agtccgcctt tcccctggct
     4681 tccgtccccg ttgaacagcg ccgctttacc tacgccctcg ccttgcctgt atctgccttg
     4741 ctcgacaaag gcgtcgatat ccaagagctt gatgtcggtg tagacgcaat cgagatcatt
     4801 gtagacgatc ttgcaacgag cgaatccggc ccaacgagcc ccttgggtct tgcgccccac
     4861 cgagcgagcg agatcagtcg tgttgtaggc gaaatcagga gggacacagt gatccccatc
     4921 attctgcacg tggtctttcc agaaagagcg ctatatgaag aagctctgct cgcgctttac
     4981 atgacttacc tgaaccatgc cttaaggctt gcgccagatt atcttacggt cgatctgggg
     5041 ctcgattctg gcttgcttgg gcaactaacc accgttcaag gaaccaccaa ggtcatcggc
     5101 aataaacaac ttgcagaggt caattcgccg cgctgggggg atccatcttg gttacaagcc
     5161 tatgaaaagg cccagaatac aggatgcgac ttggtaaggt tgaccagacc ggcttcaaat
     5221 ccccgggaca acacagacat tcggcagttc cacgttgctg tagaggccgt cgggggtcca
     5281 aggctcccat ttattgctta caacacagga cgcctaggtc ggacatcgat gtgttttaac
     5341 gagatcctga ctccagttac accagtgcct accaaggagg atgcaatcgg gctccgcaat
     5401 ccagcccatc gctatctcca gcctccgctc acggctctgg aagcaacaca ggctctctac
     5461 tcggcatttg tccacgaccc aatgaagctg tatgtctttg gcgcaaatgt gggatatagc
     5521 ttgtccccag ccatgcacaa tgccgcactc aaggcctgtg gcattccaca ccattacaag
     5581 cccctttcca cagcaaacat cgggactttg cgcgaggtta tcagcgatcc gcagtttgct
     5641 ggagcctcgg tcggcctgcc gtttaaggtg gaaatcatca gcctcacaca ctcgctgagc
     5701 cggcacgcga aagccatcgg agccgtcaac accttgattc cggtacgaca ccttaccgcg
     5761 gacggtggaa taccggacga ggtgtccatg ttcaacaata tcagccaagc cggcgctgtc
     5821 agagctctct acggcgagaa cacggattgg attggtatcc gagcctgcct tcgccgcggt
     5881 ttatcgcccg ccaatgccgt gagatcaaca agcactggtc ttgtcatcgg cgctggcgga
     5941 atggctaggg cagctgtcta tgccatgctt caactgggag tcaagaagat tttgatcttt
     6001 aaccgaacat ttgctaatgc cgagaagctg gttctacact tcgagaacct gttggtcaga
     6061 gacgcattgc ctctgttgag cacagggcca agatcccacg acaacacctg ttttcacatc
     6121 attcgatctc gagacgatcc gctcccagaa aacttcaaaa acccgaccat gatcgtttcc
     6181 tgcataccga cacacacagt ggacaacacc cctgaccctg aatttactgt gcctttgcac
     6241 tggctcgaca accccactgg cggcattgta ctagaactcg actacaaatg tctcacatca
     6301 cccttgctcg aacaaacacg acgcgaggct cacagaggct gggtcgcaat ggatggactt
     6361 gacctcttgc cagaacaagg gtttgcccaa tttgaactgt tcaccgggcg gcgagcacct
     6421 cgtcgcttga tgaggcgcga ggttttgcga gcatacccag atgatcaagc aaaatctcat
     6481 accgcgcagt tacagcctcg cctcaacgga attgcaacgc aaatatctcc atggggcagt
     6541 ggggctacta acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct
     6601 atggtctccg agctcattaa agaaaacatg catatgaagc tctacatgga gggaaccgtc
     6661 aacaaccacc acttcaagtg cacctccgag ggagagggaa agccatacga gggaacccaa
     6721 accatgcgta tcaaggccgt cgagggagga ccactcccat tcgccttcga catcctcgcc
     6781 acctccttca tgtacggatc caaggtaagt ttaaacatat atatactaac taaccctgat
     6841 tatttaaatt ttcagacctt catcaaccac acccaaggaa tcccagactt cttcaagcaa
     6901 tccttcccag agggattcac ctgggagcgt gtcaccacct acgaggacgg aggagtcctc
     6961 accgccaccc aagacacctc cctccaagac ggatgcctca tctacaacgt caaggtaagt
     7021 ttaaacagtt cggtactaac taaccataca tatttaaatt ttcagatccg tggagtcaac
     7081 ttcccatcca acggaccagt catgcaaaag aagaccctcg gatgggaggc ctccaccgag
     7141 accctctacc cagccgacgg aggacttgag ggacgtgccg acatggccct caagctcgtc
     7201 ggaggaggac acctcatctg caacctcaag gtaagtttaa acatgatttt actaactaac
     7261 taatctgatt taaattttca gaccacctac cgttccaaga agccagccaa gaacctcaag
     7321 atgccaggag tctactacgt cgaccgtcgt cttgagcgta tcaaggaggc cgacaaggag
     7381 acctacgtcg agcaacacga ggtcgccgtc gcccgttact gcgacctccc atccaagctc
     7441 ggacaccgtt gagcgccggt cgctaccatt accaacttgt ctggtgtcaa aaataatagg
     7501 ggccgctgtc atcagagtaa gtttaaactg agttctacta actaacgagt aatatttaaa
     7561 ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct
     7621 acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct
     7681 taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc
     7741 aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa
     7801 taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt
     7861 ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt
     7921 tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt
     7981 caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa
     8041 agtttatgaa attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag
     8101 atttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa
     8161 agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg
     8221 aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac
     8281 caaacataac tgtttcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt
     8341 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     8401 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     8461 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     8521 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt
     8581 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     8641 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8701 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8761 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8821 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8881 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8941 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9001 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9061 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     9121 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     9181 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     9241 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9301 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     9361 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     9421 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9481 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9541 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9601 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9661 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9721 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9781 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9841 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9901 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     9961 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10021 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10081 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10141 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc
    10201 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    10261 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10321 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10381 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10441 catgttcttt cctgcgttat cccctgattc tgtggat
//
