LOCUS       sequence-233936-        4713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of moxMaple3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Kaberniuk AA, Mohr MA, Verkhusha VV, Snapp EL
  TITLE     moxMaple3: a Photoswitchable Fluorescent Protein for PALM and 
            Protein Highlighting in Oxidizing Cellular Environments.
  JOURNAL   Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.1038/s41598-018-32955-5.
  PUBMED    30283009
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-10-3"; volume: "8(1):14738. doi"; pages: " 
            10.1038/s41598-018-32955-5"
COMMENT     Sequence Label: pBAD/HisD-moxMaple3
FEATURES             Location/Qualifiers
     source          1..4713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             328..345
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1114..1131)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1284..1370
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1462..1489
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1508..1599
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1600..1668,1669..2460)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1818..1837)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2631..3219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3120..3139
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3373..3390
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3405..3545
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3471..3490)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3631..3653
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3809..4687)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat ggggagccac catcaccatc accatggcag atctatggtg
      361 agcaagggcg aggagaccat catgagcgtg atcaagcccg acatgaagat caagctgcgc
      421 atggagggca acgtgaacgg ccacgccttc gtgatcgagg gcgagggcag cggcaagccc
      481 ttcgagggca tccagaccat cgacctggag gtgaaggagg gcgcccccct gcccttcgcc
      541 tacgacatcc tgaccaccgc cttccactac ggcaaccgcg tgttcaccaa gtacccccgc
      601 aagatccccg actacttcaa gcagagcttc cccgagggct acagctggga gcgcagcatg
      661 acctacgagg acggcggcat cgtccaggcc accaacgaca tcaccatgga ggaggacagc
      721 ttcatcaaca agatccactt caagggcacc aacttccccc ccaacggccc cgtgatgcag
      781 aagcgcaccg tgggctggga ggtgagcacc gagaagatgt acgtgcgcga cggcgtgctg
      841 aagggcgacg tgaagatgaa gctgctgctg aagggcggca gccactaccg cgccgacttc
      901 cgcaccacct acaaggtgaa gcagaaggcc gtgaagctgc ccaaggccca cttcgtggac
      961 caccgcatcg agatcctgag ccacgacaag gactacaaca aggtgaagct gtacgagcac
     1021 gccgtggccc gcgacagcac cgacagcatg gacgagctgt acaagtaaga attcgaagct
     1081 tggctgtttt ggtggatgag agaagatttt cagcctgata cagattaaat cagaacgcag
     1141 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc
     1201 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
     1261 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1321 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
     1381 cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa
     1441 ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
     1501 aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1561 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1621 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1681 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1741 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1801 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     1861 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1921 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1981 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2041 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2101 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2161 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2221 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2281 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2341 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2401 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2461 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2521 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2581 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2641 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2701 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2761 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     2821 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2881 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2941 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3001 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3061 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3121 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3181 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3241 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3301 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3361 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     3421 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     3481 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     3541 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     3601 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     3661 cgtcatcacc gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg
     3721 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
     3781 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
     3841 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
     3901 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
     3961 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
     4021 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
     4081 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
     4141 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
     4201 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
     4261 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
     4321 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
     4381 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
     4441 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
     4501 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
     4561 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
     4621 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
     4681 cagccatact tttcatactc ccgccattca gag
//
