LOCUS       sequence-233960-       13143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLP(D) with a hsp70 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13143)
  AUTHORS   Chai A, Mateus AM, Oozeer F, Sousa-Nunes R
  TITLE     Spatiotemporally controlled genetic perturbation for efficient 
            large-scale studies of cell non-autonomous effects.
  JOURNAL   Elife. 2018 Nov 27;7. pii: 38393. doi: 10.7554/eLife.38393.
  PUBMED    30479273
REFERENCE   2  (bases 1 to 13143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-11-27"; volume: "7. pii: 38393. doi"; pages: " 
            10.7554/eLife.38393"
COMMENT     Sequence Label: pDEST-Hemmar-FLP(D)
FEATURES             Location/Qualifiers
     source          1..13143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    631..1016
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    1050..1174
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1199..1204,1205..1222,1223..1229)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1283..1942
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1349..1368)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2284..2589
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2468..2487
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2630..2754)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    4997..5382
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(5392..5410)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5862..5933,6335..6608,6683..7337,7399..7714,7918..8049,
                     8120..8734)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9513..10067,10068..10098)
                     /label=P element 5' end
     rep_origin      complement(10333..10921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10413..10432)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11092..11883,11884..11952))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11715..11734
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11953..12057)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12125..12143
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12181..12203)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12303..12322
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    12531..12600
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttggctgcat cacgtaataa gtgtgcgttg aatttattcg
      661 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
      721 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
      781 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
      841 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
      901 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
      961 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     1021 tcggcaacag catgctgcag cagatcatca caagtttgta caaaaaagct gaacgagaaa
     1081 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1141 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     1201 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     1261 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     1321 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     1381 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     1441 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     1501 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     1561 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     1621 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     1681 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     1741 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     1801 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     1861 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1921 tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta
     1981 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     2041 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     2101 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     2161 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     2221 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     2281 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     2341 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     2401 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     2461 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     2521 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     2581 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     2641 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     2701 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgatgat
     2761 ctggtctagc gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
     2821 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
     2881 tgagcaagct aaacaatcgt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca
     2941 accaagtaaa tcaactgcaa ctactgaaat ctgccggtga gattgtacac ccttcttaaa
     3001 gatccccttc aaagtctaaa ttcttccttt tcgtttagcc aagactcgag aatcaaaatg
     3061 ccgcagtttg atatcctctg caagacccca ccaaaggtgt tggtgcgtca attcgtggag
     3121 cgatttgaga ggccgtcggg tgagaagatc gccctgtgcg ctgccgagtt gacctatttg
     3181 tgttggatga tcactcataa tggcaccgcg attaagcgcg ctacctttat gagctataac
     3241 actatcatta gcaattccct gtcctttgac atagtaaaca agtccctgca gtttaaatac
     3301 aagactcaga aggccactat attggaggct tcgctgaaaa agttgatccc ggcatgggag
     3361 ttcacgatca tcccatacta cggtcagaaa caccagagcg atattaccga tattgtaagc
     3421 agcctccagc tgcagtttga gtccagcgaa gaggctgata agggtaatag tcacagcaaa
     3481 aagatgctga aggcactgct gtccgagggc gaaagcatct gggagattac tgagaaaatc
     3541 ctgaactcgt ttgagtacac cagccgattc accaagacga agaccctgta ccagttcctc
     3601 tttttggcaa ccttcatcaa ttgtggtcgc ttcagtgaca tcaaaaacgt ggaccctaaa
     3661 tcgttcaagc tggtgcagaa taagtacctg ggtgttatca tccagtgctt ggttaccgag
     3721 actaagacct ccgtgtccag gcacatttat ttcttctccg caaggggtcg catcgatccg
     3781 ctggtatact tggatgagtt ccttcggaat agtgaaccag tcttgaagcg cgtgaacagg
     3841 acgggcaatt ccagtagcaa caagcaagag taccagctgc tgaaggataa tcttgttcgg
     3901 tcgtacaaca aagccttgaa gaaaaatgcg ccatactcga tctttgcaat caagaatggt
     3961 cccaagagtc atattggccg gcacctgatg accagtttct tgtccatgaa gggtctgact
     4021 gagctcacaa acgtcgttgg caactggtcg gacaagcgag cgagtgccgt cgcccgcacg
     4081 acatacaccc accagatcac ggccatacca gatcactatt tcgcgctggt gtcgcgttat
     4141 tatgcctatg atcccatcag caaggagatg atcgcgctga aggacgaaac caatccaatc
     4201 gaggagtggc agcatatcga gcaacttaag ggaagcgctg agggtagcat ccgttacccc
     4261 gcctggaacg gcatcatcag ccaggaggtt ctggattacc tgagctccta catcaatcgc
     4321 cgtatttaat ctagaattaa ttaactaagc cagtcggcaa tcggacgcct tcgaaacatg
     4381 caacactaat gtttaattca gatttcagca gagacaagct aaaacaccga cgagttgtaa
     4441 tcatttctgt gcgccagcat atatttctta tatacaacgt aatacataat tatgtaattc
     4501 tagcatctcc ccaacactca catacaaaca aacaaaaaat acaaacacac aaaacgtatt
     4561 tacccgcacg catccttggc gaggttgagt atgaaacaaa aacaaaactt aatttagagc
     4621 aaagtaatta cacgaataaa tttaataaaa aaaaactata ataaaaacgc caatcatgtt
     4681 atttccaaaa aaaaaaaaaa aaaaaaaact agacgagatg tttgcgcttg tgtcgatgaa
     4741 tgtaaagaat tgcaactaca tagatggtaa ttaatttcag ctgtaagaag tacaagtttt
     4801 tcataattgt gagaaaagtg cagtagtatc gcttaaagtt cagttttaat cagggcattt
     4861 agcagctcga acccaatgta tttgtaagcc caataaacgt gtaaatgcct agagcaaaat
     4921 atcacaactg tagcttttca tccgaaagat aataatgaat agactgacct gacgtttttc
     4981 aaaatgcgta actagtcacg taataagtgt gcgttgaatt tattcgcaaa aacattgcat
     5041 attttcggca aagtaaaatt ttgttgcata ccttatcaaa aaataagtgc tgcatacttt
     5101 ttagagaaac caaataattt tttattgcat acccgttttt aataaaatac attgcatacc
     5161 ctcttttaat aaaaaatatt gcatactttg acgaaacaaa ttttcgttgc atacccaata
     5221 aaagattatt atattgcata cccgttttta ataaaataca ttgcataccc tcttttaata
     5281 aaaaatattg catacgttga cgaaacaaat tttcgttgca tacccaataa aagattatta
     5341 tattgcatac cttttcttgc cataccattt agccgatcaa ttctagtatg tatgtaagtt
     5401 aataaaaccc atttttgcgg aaagtagata aaaaaaacat tttttttttt tactgcactg
     5461 gatatcattg aacttatctg atcagtttta aatttacttc gatccaaggg tatttgatgt
     5521 accaggttct ttcgattacc tctcactcaa aatgacattc cactcaaagt cagcgctgtt
     5581 tgcctccttc tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc
     5641 tttcgccacc gtttgtagcg ttacgtagcg tcaatgtccg ccttcagttg cattttgtca
     5701 gcggtttcgt gacgaagctc caagcggttt acgccatcaa ttaaacacaa agtgctgtgc
     5761 caaaactcct ctcgcttctt atttttgttt gttttttgag tgattggggt ggtgattggt
     5821 tttgggtggg taagcagggg aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg
     5881 agctattaat tcgcggaggc agcaaacacc catctgccga gcatctgaac aatgtgagta
     5941 gtacatgtgc atacatctta agttcacttg atctatagga actgcgattg caacatcaaa
     6001 ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa accgcaattg cacaaacaaa
     6061 tagtgacacg aaacagatta ttctggtagc tgttctcgct atataagaca atttttgaga
     6121 tcatatcatg atcaagacat ctaaaggcat tcattttcga ctatattctt ttttacaaaa
     6181 aatataacaa ccagatattt taagctgatc ctagatgcac aaaaaataaa taaaagtata
     6241 aacctacttc gtaggatact tcggggtact ttttgttcgg ggttagatga gcataacgct
     6301 tgtagttgat atttgagatc ccctatcatt gcagggtgac agcggagcgg cttcgcagag
     6361 ctgcattaac cagggcttcg ggcaggccaa aaactacggc acgctccggc cacccagtcc
     6421 gccggaggac tccggttcag ggagcggcca actagccgag aacctcacct atgcctggca
     6481 caatatggac atctttgggg cggtcaatca gccgggctcc ggatggcggc agctggtcaa
     6541 ccggacacgc ggactattct gcaacgagcg acacataccg gcgcccagga aacatttgct
     6601 caagaacggt gagtttctat tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc
     6661 caattaccaa tttgaaactc agtttgcggc gtggcctatc cgggcgaact tttggccgtg
     6721 atgggcagtt ccggtgccgg aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg
     6781 cagggcatcc aagtatcgcc atccgggatg cgactgctca atggccaacc tgtggacgcc
     6841 aaggagatgc aggccaggtg cgcctatgtc cagcaggatg acctctttat cggctcccta
     6901 acggccaggg aacacctgat tttccaagcc atggtgcgga tgccacgaca tctgacctat
     6961 cggcagcgag tggcccgcgt ggatcaggtg atccaggagc tttcgctcag caaatgtcag
     7021 cacacgatca tcggtgtgcc cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt
     7081 ctggcattcg cctccgaggc tctaaccgat ccgccgcttc tgatctgcga tgagcccacc
     7141 tccggactgg actcctttac cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag
     7201 aagggcaaga ccgtcatcct gaccattcat cagccgtctt ccgagctgtt tgagctcttt
     7261 gacaagatcc ttctgatggc cgagggcagg gtagctttct tgggcactcc cagcgaagcc
     7321 gtcgacttct tttcctagtg agttcgatgt gtttattaag ggtatctagt attacataac
     7381 atctcaactc ctatccagcg tgggtgccca gtgtcctacc aactacaatc cggcggactt
     7441 ttacgtacag gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc
     7501 caagatatgc gacaattttg ccattagcaa agtagcccgg gatatggagc agttgttggc
     7561 caccaaaaat ctggagaagc cactggagca gccggagaat gggtacacct acaaggccac
     7621 ctggttcatg cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc
     7681 actcctcgta aaagtgcgac ttattcagac aacggtgagt ggttccagtg gaaacaaatg
     7741 atataacgct tacaattctt ggaaacaaat tcgctagatt ttagatagaa ttgcctgatt
     7801 ccacaccctt cttagttttt ttcaatgaga tgtatagttt atagttttgc agaagataaa
     7861 taaatttcat ttaactcgcg aatattaatg agatgcgagt aacattttaa tttgcagatg
     7921 gttgccatct tgattggcct catctttttg ggccaacaac tcacgcaagt gggtgtgatg
     7981 aatatcaacg gagccatctt cctcttcctg accaacatga cctttcaaaa cgtctttgcc
     8041 acgataaatg taagtcatgt ttagaataca tttgcatttc aataatttac taactttcta
     8101 atgaatcgat tcgatttagg tgttcacctc agagctgcca gtttttatga gggaggcccg
     8161 aagtcgactt tatcgctgtg acacatactt tctgggcaaa acgattgccg aattgccgct
     8221 ttttctcaca gtgccactgg tcttcacggc gattgcctat ccgatgatcg gactgcgggc
     8281 cggagtgctg cacttcttca actgcctggc gctggtcact ctggtggcca atgtgtcaac
     8341 gtccttcgga tatctaatat cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg
     8401 tccgccggtt atcataccat tcctgctctt tggcggcttc ttcttgaact cgggctcggt
     8461 gccagtatac ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca acgagggtct
     8521 gctgattaac caatgggcgg acgtggagcc gggcgaaatt agctgcacat cgtcgaacac
     8581 cacgtgcccc agttcgggca aggtcatcct ggagacgctt aacttctccg ccgccgatct
     8641 gccgctggac tacgtgggtc tggccattct catcgtgagc ttccgggtgc tcgcatatct
     8701 ggctctaaga cttcgggccc gacgcaagga gtagccgaca tatatccgaa ataactgctt
     8761 gttttttttt tttaccatta ttaccatcgt gtttactgtt tattgccccc tcaaaaagct
     8821 aatgtaatta tatttgtgcc aataaaaaca agatatgacc tatagaatac aagtatttcc
     8881 ccttcgaaca tccccacaag tagactttgg atttgtcttc taaccaaaag acttacacac
     8941 ctgcatacct tacatcaaaa actcgtttat cgctacataa aacaccggga tatatttttt
     9001 atatacatac ttttcaaatc gcgcgccctc ttcataattc acctccacca caccacgttt
     9061 cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa cacattcacc ttttgttcga
     9121 cgaccttgga gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat ggttccgagt
     9181 ggttcatttc gtctcaatag aaattagtaa taaatatttg tatgtacaat ttatttgctc
     9241 caatatattt gtatatattt ccctcacagc tatatttatt ctaatttaat attatgactt
     9301 tttaaggtaa ttttttgtga cctgttcgga gtgattagcg ttacaatttg aactgaaagt
     9361 gacatccagt gtttgttcct tgtgtagatg catctcaaaa aaatggtggg cataatagtg
     9421 ttgtttatat atatcaaaaa taacaactat aataataaga atacatttaa tttagaaaat
     9481 gcttggattt cactggaact agaattaatt cggctgctgc tctaaacgac gcatttcgta
     9541 ctccaaagta cgaatttttt ccctcaagct cttattttca ttaaacaatg aacaggacct
     9601 aacgcacagt cacgttattg tttacataaa tgattttttt tactattcaa acttactctg
     9661 tttgtgtact cccactggta tagccttctt ttatcttttc tggttcaggc tctatcactt
     9721 tactaggtac ggcatctgcg ttgagtcgcc tccttttaaa tgtctgacct tttgcaggtg
     9781 cagccttcca ctgcgaatca ttaaagtggg tatcacaaat ttgggagttt tcaccaaggc
     9841 tgcacccaag gctctgctcc cacaattttc tcttaatagc acacttcggc acgtgaatta
     9901 attttactcc agtcacagct ttgcagcaaa atttgcaata tttcattttt ttttattcca
     9961 cgtaagggtt aatgttttca aaaaaaaatt cgtccgcaca caacctttcc tctcaacaag
    10021 caaacgtgca ctgaatttaa gtgtatactt cggtaagctt cggctatcga cgggaccacc
    10081 ttatgttatt tcatcatggg ccagacccac gtagtccagc ggcagatcgg cggcggagaa
    10141 gttaagcgtc tccaggatga ccttgcccga actggggcac gtggtgttcg acgatgtgca
    10201 gctaatttcg cccggctcca cgtccgccca ttggttaatc agcagaccct cgttggcgta
    10261 acggaaccat gagaggtacg acaaccattt gaggtatact ggcaccgagc ccgagttcaa
    10321 gaagaaggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10381 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10441 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10501 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10561 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10621 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10681 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10741 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    10801 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10861 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10921 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10981 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    11041 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    11101 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    11161 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    11221 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    11281 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11341 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11401 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11461 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11521 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11581 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11641 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11701 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11761 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11821 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11881 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11941 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    12001 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    12061 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    12121 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    12181 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    12241 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    12301 tgcggcatca gagcagattg tactgagagt gcaccattcg acatgcccgc cgtgaccgtc
    12361 gagaacccgc tgacgctgcc ccgcgtatcc gcacccgccg acgccgtcgc acgtcccgtg
    12421 ctcaccgtga ccaccgcgcc cagcggtttc gagggcgagg gcttcccggt gcgccgcgcg
    12481 ttcgccggga tcaactaccg ccacctcgac ccgttcatca tgatggacca gatgggtgag
    12541 gtggagtacg cgcccgggga gcccaagggc acgccctggc acccgcaccg cggcttcgag
    12601 accgtgacct acatcgtcga tatgcggtgt gaaataccgc accgaatcgc gcggaactaa
    12661 cgacagtcgc tccaaggtcg tcgaacaaaa ggtgaatgtg ttgcggagag cgggtgggag
    12721 acagcgaaag agcaactacg aaacgtggtg tggtggaggt gaattatgaa gagggcgcgc
    12781 gatttgaaaa gtatgtatat aaaaaatata tcccggtgtt ttatgtagcg ataaacgagt
    12841 ttttgatgta aggtatgcag gtgtgtaagt cttttggtta gaagacaaat ccaaagtcta
    12901 cttgtgggga tgttcgaagg ggaaatactt gtattctata ggtcatatct tgtttttatt
    12961 ggcacaaata taattacatt agctttttga gggggcaata aacagtaaac acgatggtaa
    13021 taatggtaaa aaaaaaaaac aagcagttat ttcggatata tgtcggctac tccttgcgtc
    13081 gggcccgaag tcttagagcc agatatgcga gcacccggaa gctcacgatg agaatggcca
    13141 gac
//
