LOCUS       sequence-233967-        3466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BglFusion compatible IPTG inducible integration vector for 
            Streptococcus pneumoniae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Keller LE, Rueff AS, Kurushima J, Veening JW
  TITLE     Three New Integration Vectors and Fluorescent Proteins for Use in 
            the Opportunistic Human Pathogen Streptococcus pneumoniae.
  JOURNAL   Genes (Basel). 2019 May 22;10(5). pii: genes10050394. doi: 
            10.3390/genes10050394.
  PUBMED    31121970
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2019-05-22"; volume: "10(5). pii: genes10050394. 
            doi"; pages: " 10.3390/genes10050394"
COMMENT     Sequence Label: pPEPY-Plac
FEATURES             Location/Qualifiers
     source          1..3466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    336..355
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     rep_origin      complement(1330..1918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1410..1429)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2548..3342)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ggtctccgat cgtgtactgc agaaaactag tcgcaaaaaa ccccgcttcg gcggggtttt
       61 ttcgctctag acggtgatca acacgctagc gcgttgatgc aatttctatg cgcacccgtt
      121 ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga
      181 gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcta tcgatgcatg
      241 cagccggtac cactagtaat tgtgaggcgg ataacaattc tcactcattc tacagtttat
      301 tcttgacatt gcactgtccc cctggtataa taactaattg tgagcgctca caattgaatt
      361 catgagatct aaataaataa ggaggaaaat taatgccgga tccctccagt aactcgagaa
      421 agtgtaagca attctgcagt actaggacgc cgccaagcca gcttaaaccc agctcaatga
      481 gctgggtttt ttgtttgtta aaaatgaaga agaaactgtg aagcgtattt atagcaaagc
      541 actcaaaagt ttaccttatg ggtgcttttt tcgtgctttt ttgaaaagac aaaaaaaaga
      601 accttgccaa gcaagattct tttgatagcg cagttgagca gtgtctttag taaatttagg
      661 gacttgtttt gtcatagttt tgatacctgt attcgttgtt tcatgagcat ttgacgtttc
      721 ttttcatgta ggcatttcaa caattagaat gatatttttc cgaggatgaa cttatatatt
      781 gtgacttttt agagttatga tgcctcttga tttgactatc gctatcaatt tcatccactt
      841 ttacagtttc atggttttat gaaccgaaac atctaggtac aggttttagc atcaattgat
      901 tgtttttgag ccatatgaag ttgaaacgtg gagatacttt tcttgtgata acgccctatt
      961 caaagaggaa taaccgtttt tggagagagt tcagtactat gtggcgctaa atactatatg
     1021 taaaacatat attaaacaag atttaactaa ggagaattgt aaaaacaaaa atgagaattt
     1081 catataaagt ttattctagg agtatgtatc ttgaataaaa attgttattt aagttctggg
     1141 ggttatgatg aatgttttta tgcggccgcg ggataacgca ggaaagaaca tgtgagcaaa
     1201 aggcataaaa acattcatca taacccccag aacttaaata acgcggccgc gggataacgc
     1261 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1321 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1381 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1501 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1741 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     1801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1861 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1921 agatcctttg atcttttcta cggggtggta ccaaacctta ctaaagtata taatttaggc
     1981 agtcaattct gtatgataat gtttgtacta tcgtcgagtt tttgcttgat tgcctaacga
     2041 gataaatttt agaagagttt tcggtgtgat ttgacaagtt tctgactgga aggatagtta
     2101 cactagtatt gtttattaaa atgagcagat agagaaaatt tggaatctat ttgcttatta
     2161 tcaatcgttt ttttagctta tttgtcttag attggtatag aactgagcta tggattaatc
     2221 ttcttctatc ttatcctgtt cctaaatatg ttggtagaaa aatgaagcag ttgttacgag
     2281 tgtctgtatt ggaaaaaaat tttgtctata caattcttta aatgatatga agactaggaa
     2341 tgccaaaaca ctgacatatt tcgtttaaag cctatccagt ctcttcaatt gtatttgtag
     2401 aaatgctatc agtaatatat tcgcgaacac tatgatttac aacagtgtgg tcgtacatct
     2461 ttgtaacttt attatacagc ttctagcaaa aaactggacg gagtcccgtc cagaaaagta
     2521 agcacttgtt attgcaataa aattagccta aaacaattca tccagtaaaa tataatattt
     2581 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     2641 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
     2701 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
     2761 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
     2821 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
     2881 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
     2941 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
     3001 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
     3061 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
     3121 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
     3181 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
     3241 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
     3301 gatcagtttt ttcaattccg gtgatattct cattttagcc attaattttc ctccttattt
     3361 atttagatcc ttgcatgtat agttattata ccagggggac agtgcaatgt caagctgaaa
     3421 tcttcgagca gaagaacctt gccaagcaag attcttttga tagggt
//
