LOCUS       sequence-233971-       13267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13267)
  AUTHORS   Chai A, Mateus AM, Oozeer F, Sousa-Nunes R
  TITLE     Spatiotemporally controlled genetic perturbation for efficient 
            large-scale studies of cell non-autonomous effects.
  JOURNAL   Elife. 2018 Nov 27;7. pii: 38393. doi: 10.7554/eLife.38393.
  PUBMED    30479273
REFERENCE   2  (bases 1 to 13267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-11-27"; volume: "7. pii: 38393. doi"; pages: " 
            10.7554/eLife.38393"
COMMENT     Sequence Label: pDEST-Hemmar-DSCP-ftz-FLP(D)
FEATURES             Location/Qualifiers
     source          1..13267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    631..1016
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    1050..1174
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1199..1204,1205..1222,1223..1229)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1283..1942
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1349..1368)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2284..2589
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2468..2487
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2630..2754)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    5121..5506
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(5516..5534)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5986..6057,6459..6732,6807..7461,7523..7838,8042..8173,
                     8244..8858)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9637..10191,10192..10222)
                     /label=P element 5' end
     rep_origin      complement(10457..11045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10537..10556)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11216..12007,12008..12076))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11839..11858
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12077..12181)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12249..12267
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12305..12327)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12427..12446
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    12655..12724
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttggctgcat cacgtaataa gtgtgcgttg aatttattcg
      661 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
      721 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
      781 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
      841 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
      901 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
      961 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     1021 tcggcaacag catgctgcag cagatcatca caagtttgta caaaaaagct gaacgagaaa
     1081 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1141 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     1201 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     1261 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     1321 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     1381 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     1441 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     1501 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     1561 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     1621 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     1681 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     1741 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     1801 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     1861 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1921 tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta
     1981 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     2041 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     2101 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     2161 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     2221 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     2281 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     2341 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     2401 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     2461 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     2521 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     2581 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     2641 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     2701 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgatgat
     2761 ctggggccgg ccgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg
     2821 ctgagagcat cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta
     2881 atatcctttg aataagccaa ctttgaatca caagacgcat accaaacggt accgcggccg
     2941 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagccta ggttggcatc
     3001 aggtaggcat cacacacgat taacaacccc taaaaataca ctttgaaaat attgaaaata
     3061 tgtttttgta tacatttttg atattttcaa ataatacgca gttataaaac tcattagcta
     3121 acccattttt tctttgctta tgcttacaga ttgcaaagac ctgcaggctc gagaatcaaa
     3181 atgccgcagt ttgatatcct ctgcaagacc ccaccaaagg tgttggtgcg tcaattcgtg
     3241 gagcgatttg agaggccgtc gggtgagaag atcgccctgt gcgctgccga gttgacctat
     3301 ttgtgttgga tgatcactca taatggcacc gcgattaagc gcgctacctt tatgagctat
     3361 aacactatca ttagcaattc cctgtccttt gacatagtaa acaagtccct gcagtttaaa
     3421 tacaagactc agaaggccac tatattggag gcttcgctga aaaagttgat cccggcatgg
     3481 gagttcacga tcatcccata ctacggtcag aaacaccaga gcgatattac cgatattgta
     3541 agcagcctcc agctgcagtt tgagtccagc gaagaggctg ataagggtaa tagtcacagc
     3601 aaaaagatgc tgaaggcact gctgtccgag ggcgaaagca tctgggagat tactgagaaa
     3661 atcctgaact cgtttgagta caccagccga ttcaccaaga cgaagaccct gtaccagttc
     3721 ctctttttgg caaccttcat caattgtggt cgcttcagtg acatcaaaaa cgtggaccct
     3781 aaatcgttca agctggtgca gaataagtac ctgggtgtta tcatccagtg cttggttacc
     3841 gagactaaga cctccgtgtc caggcacatt tatttcttct ccgcaagggg tcgcatcgat
     3901 ccgctggtat acttggatga gttccttcgg aatagtgaac cagtcttgaa gcgcgtgaac
     3961 aggacgggca attccagtag caacaagcaa gagtaccagc tgctgaagga taatcttgtt
     4021 cggtcgtaca acaaagcctt gaagaaaaat gcgccatact cgatctttgc aatcaagaat
     4081 ggtcccaaga gtcatattgg ccggcacctg atgaccagtt tcttgtccat gaagggtctg
     4141 actgagctca caaacgtcgt tggcaactgg tcggacaagc gagcgagtgc cgtcgcccgc
     4201 acgacataca cccaccagat cacggccata ccagatcact atttcgcgct ggtgtcgcgt
     4261 tattatgcct atgatcccat cagcaaggag atgatcgcgc tgaaggacga aaccaatcca
     4321 atcgaggagt ggcagcatat cgagcaactt aagggaagcg ctgagggtag catccgttac
     4381 cccgcctgga acggcatcat cagccaggag gttctggatt acctgagctc ctacatcaat
     4441 cgccgtattt aatctagaat taattaacta agccagtcgg caatcggacg ccttcgaaac
     4501 atgcaacact aatgtttaat tcagatttca gcagagacaa gctaaaacac cgacgagttg
     4561 taatcatttc tgtgcgccag catatatttc ttatatacaa cgtaatacat aattatgtaa
     4621 ttctagcatc tccccaacac tcacatacaa acaaacaaaa aatacaaaca cacaaaacgt
     4681 atttacccgc acgcatcctt ggcgaggttg agtatgaaac aaaaacaaaa cttaatttag
     4741 agcaaagtaa ttacacgaat aaatttaata aaaaaaaact ataataaaaa cgccaatcat
     4801 gttatttcca aaaaaaaaaa aaaaaaaaaa aactagacga gatgtttgcg cttgtgtcga
     4861 tgaatgtaaa gaattgcaac tacatagatg gtaattaatt tcagctgtaa gaagtacaag
     4921 tttttcataa ttgtgagaaa agtgcagtag tatcgcttaa agttcagttt taatcagggc
     4981 atttagcagc tcgaacccaa tgtatttgta agcccaataa acgtgtaaat gcctagagca
     5041 aaatatcaca actgtagctt ttcatccgaa agataataat gaatagactg acctgacgtt
     5101 tttcaaaatg cgtaactagt cacgtaataa gtgtgcgttg aatttattcg caaaaacatt
     5161 gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata
     5221 ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca
     5281 taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc
     5341 aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat accctctttt
     5401 aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt
     5461 attatattgc ataccttttc ttgccatacc atttagccga tcaattctag tatgtatgta
     5521 agttaataaa acccattttt gcggaaagta gataaaaaaa acattttttt tttttactgc
     5581 actggatatc attgaactta tctgatcagt tttaaattta cttcgatcca agggtatttg
     5641 atgtaccagg ttctttcgat tacctctcac tcaaaatgac attccactca aagtcagcgc
     5701 tgtttgcctc cttctctgtc cacagaaata tcgccgtctc tttcgccgct gcgtccgcta
     5761 tctctttcgc caccgtttgt agcgttacgt agcgtcaatg tccgccttca gttgcatttt
     5821 gtcagcggtt tcgtgacgaa gctccaagcg gtttacgcca tcaattaaac acaaagtgct
     5881 gtgccaaaac tcctctcgct tcttattttt gtttgttttt tgagtgattg gggtggtgat
     5941 tggttttggg tgggtaagca ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat
     6001 caggagctat taattcgcgg aggcagcaaa cacccatctg ccgagcatct gaacaatgtg
     6061 agtagtacat gtgcatacat cttaagttca cttgatctat aggaactgcg attgcaacat
     6121 caaattgtct gcggcgtgag aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa
     6181 caaatagtga cacgaaacag attattctgg tagctgttct cgctatataa gacaattttt
     6241 gagatcatat catgatcaag acatctaaag gcattcattt tcgactatat tcttttttac
     6301 aaaaaatata acaaccagat attttaagct gatcctagat gcacaaaaaa taaataaaag
     6361 tataaaccta cttcgtagga tacttcgggg tactttttgt tcggggttag atgagcataa
     6421 cgcttgtagt tgatatttga gatcccctat cattgcaggg tgacagcgga gcggcttcgc
     6481 agagctgcat taaccagggc ttcgggcagg ccaaaaacta cggcacgctc cggccaccca
     6541 gtccgccgga ggactccggt tcagggagcg gccaactagc cgagaacctc acctatgcct
     6601 ggcacaatat ggacatcttt ggggcggtca atcagccggg ctccggatgg cggcagctgg
     6661 tcaaccggac acgcggacta ttctgcaacg agcgacacat accggcgccc aggaaacatt
     6721 tgctcaagaa cggtgagttt ctattcgcag tcggctgatc tgtgtgaaat cttaataaag
     6781 ggtccaatta ccaatttgaa actcagtttg cggcgtggcc tatccgggcg aacttttggc
     6841 cgtgatgggc agttccggtg ccggaaagac gaccctgctg aatgcccttg cctttcgatc
     6901 gccgcagggc atccaagtat cgccatccgg gatgcgactg ctcaatggcc aacctgtgga
     6961 cgccaaggag atgcaggcca ggtgcgccta tgtccagcag gatgacctct ttatcggctc
     7021 cctaacggcc agggaacacc tgattttcca agccatggtg cggatgccac gacatctgac
     7081 ctatcggcag cgagtggccc gcgtggatca ggtgatccag gagctttcgc tcagcaaatg
     7141 tcagcacacg atcatcggtg tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa
     7201 gcgtctggca ttcgcctccg aggctctaac cgatccgccg cttctgatct gcgatgagcc
     7261 cacctccgga ctggactcct ttaccgccca cagcgtcgtc caggtgctga agaagctgtc
     7321 gcagaagggc aagaccgtca tcctgaccat tcatcagccg tcttccgagc tgtttgagct
     7381 ctttgacaag atccttctga tggccgaggg cagggtagct ttcttgggca ctcccagcga
     7441 agccgtcgac ttcttttcct agtgagttcg atgtgtttat taagggtatc tagtattaca
     7501 taacatctca actcctatcc agcgtgggtg cccagtgtcc taccaactac aatccggcgg
     7561 acttttacgt acaggtgttg gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga
     7621 tcgccaagat atgcgacaat tttgccatta gcaaagtagc ccgggatatg gagcagttgt
     7681 tggccaccaa aaatctggag aagccactgg agcagccgga gaatgggtac acctacaagg
     7741 ccacctggtt catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg
     7801 aaccactcct cgtaaaagtg cgacttattc agacaacggt gagtggttcc agtggaaaca
     7861 aatgatataa cgcttacaat tcttggaaac aaattcgcta gattttagat agaattgcct
     7921 gattccacac ccttcttagt ttttttcaat gagatgtata gtttatagtt ttgcagaaga
     7981 taaataaatt tcatttaact cgcgaatatt aatgagatgc gagtaacatt ttaatttgca
     8041 gatggttgcc atcttgattg gcctcatctt tttgggccaa caactcacgc aagtgggtgt
     8101 gatgaatatc aacggagcca tcttcctctt cctgaccaac atgacctttc aaaacgtctt
     8161 tgccacgata aatgtaagtc atgtttagaa tacatttgca tttcaataat ttactaactt
     8221 tctaatgaat cgattcgatt taggtgttca cctcagagct gccagttttt atgagggagg
     8281 cccgaagtcg actttatcgc tgtgacacat actttctggg caaaacgatt gccgaattgc
     8341 cgctttttct cacagtgcca ctggtcttca cggcgattgc ctatccgatg atcggactgc
     8401 gggccggagt gctgcacttc ttcaactgcc tggcgctggt cactctggtg gccaatgtgt
     8461 caacgtcctt cggatatcta atatcctgcg ccagctcctc gacctcgatg gcgctgtctg
     8521 tgggtccgcc ggttatcata ccattcctgc tctttggcgg cttcttcttg aactcgggct
     8581 cggtgccagt atacctcaaa tggttgtcgt acctctcatg gttccgttac gccaacgagg
     8641 gtctgctgat taaccaatgg gcggacgtgg agccgggcga aattagctgc acatcgtcga
     8701 acaccacgtg ccccagttcg ggcaaggtca tcctggagac gcttaacttc tccgccgccg
     8761 atctgccgct ggactacgtg ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat
     8821 atctggctct aagacttcgg gcccgacgca aggagtagcc gacatatatc cgaaataact
     8881 gcttgttttt tttttttacc attattacca tcgtgtttac tgtttattgc cccctcaaaa
     8941 agctaatgta attatatttg tgccaataaa aacaagatat gacctataga atacaagtat
     9001 ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca aaagacttac
     9061 acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc gggatatatt
     9121 ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc accacaccac
     9181 gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt caccttttgt
     9241 tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc
     9301 gagtggttca tttcgtctca atagaaatta gtaataaata tttgtatgta caatttattt
     9361 gctccaatat atttgtatat atttccctca cagctatatt tattctaatt taatattatg
     9421 actttttaag gtaatttttt gtgacctgtt cggagtgatt agcgttacaa tttgaactga
     9481 aagtgacatc cagtgtttgt tccttgtgta gatgcatctc aaaaaaatgg tgggcataat
     9541 agtgttgttt atatatatca aaaataacaa ctataataat aagaatacat ttaatttaga
     9601 aaatgcttgg atttcactgg aactagaatt aattcggctg ctgctctaaa cgacgcattt
     9661 cgtactccaa agtacgaatt ttttccctca agctcttatt ttcattaaac aatgaacagg
     9721 acctaacgca cagtcacgtt attgtttaca taaatgattt tttttactat tcaaacttac
     9781 tctgtttgtg tactcccact ggtatagcct tcttttatct tttctggttc aggctctatc
     9841 actttactag gtacggcatc tgcgttgagt cgcctccttt taaatgtctg accttttgca
     9901 ggtgcagcct tccactgcga atcattaaag tgggtatcac aaatttggga gttttcacca
     9961 aggctgcacc caaggctctg ctcccacaat tttctcttaa tagcacactt cggcacgtga
    10021 attaatttta ctccagtcac agctttgcag caaaatttgc aatatttcat ttttttttat
    10081 tccacgtaag ggttaatgtt ttcaaaaaaa aattcgtccg cacacaacct ttcctctcaa
    10141 caagcaaacg tgcactgaat ttaagtgtat acttcggtaa gcttcggcta tcgacgggac
    10201 caccttatgt tatttcatca tgggccagac ccacgtagtc cagcggcaga tcggcggcgg
    10261 agaagttaag cgtctccagg atgaccttgc ccgaactggg gcacgtggtg ttcgacgatg
    10321 tgcagctaat ttcgcccggc tccacgtccg cccattggtt aatcagcaga ccctcgttgg
    10381 cgtaacggaa ccatgagagg tacgacaacc atttgaggta tactggcacc gagcccgagt
    10441 tcaagaagaa ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    10501 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10561 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10621 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10681 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10741 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10801 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10861 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10921 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10981 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    11041 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    11101 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    11161 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    11221 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    11281 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    11341 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    11401 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    11461 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    11521 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    11581 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11641 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11701 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11761 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11821 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11881 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11941 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    12001 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    12061 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    12121 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    12181 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    12241 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
    12301 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    12361 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
    12421 actatgcggc atcagagcag attgtactga gagtgcacca ttcgacatgc ccgccgtgac
    12481 cgtcgagaac ccgctgacgc tgccccgcgt atccgcaccc gccgacgccg tcgcacgtcc
    12541 cgtgctcacc gtgaccaccg cgcccagcgg tttcgagggc gagggcttcc cggtgcgccg
    12601 cgcgttcgcc gggatcaact accgccacct cgacccgttc atcatgatgg accagatggg
    12661 tgaggtggag tacgcgcccg gggagcccaa gggcacgccc tggcacccgc accgcggctt
    12721 cgagaccgtg acctacatcg tcgatatgcg gtgtgaaata ccgcaccgaa tcgcgcggaa
    12781 ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa tgtgttgcgg agagcgggtg
    12841 ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg aggtgaatta tgaagagggc
    12901 gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg tgttttatgt agcgataaac
    12961 gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg gttagaagac aaatccaaag
    13021 tctacttgtg gggatgttcg aaggggaaat acttgtattc tataggtcat atcttgtttt
    13081 tattggcaca aatataatta cattagcttt ttgagggggc aataaacagt aaacacgatg
    13141 gtaataatgg taaaaaaaaa aaacaagcag ttatttcgga tatatgtcgg ctactccttg
    13201 cgtcgggccc gaagtcttag agccagatat gcgagcaccc ggaagctcac gatgagaatg
    13261 gccagac
//
