LOCUS       sequence-233976-        2715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2715)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U6-MosIR
FEATURES             Location/Qualifiers
     source          1..2715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(289..307)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        375..479
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(480..548,549..1340)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(698..717)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1511..2099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2000..2019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2253..2270
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2370..2618
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2370..2390
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2541..2560
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 cagaaactcc gctgggatgc acgtaggtcc ttggtgcacc atcaagcaag taaacaagaa
       61 aggggggtaa acacagactg gggtagatgg ataaataatc tggtttacca aagttttact
      121 cttttggaat cacccttgca ccaaggacct acgtgcatcc cagcggagtt tctgggctga
      181 actgaacatt gcaagactac gccacgacaa catagttcgg gttgtggctg ccagcacgcg
      241 cacgcccgaa gactccaaca gcctaggtac cttttttctg caggggcggc ctcgtgatac
      301 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      361 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      421 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      481 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      541 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      601 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      661 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      721 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      781 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      841 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      901 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      961 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1021 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1081 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1141 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1201 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1261 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1321 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1381 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1441 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1501 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1561 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1621 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1681 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1741 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1801 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1861 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1921 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1981 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2041 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2101 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2161 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2221 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     2281 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     2341 acgacaggtt tcccgactgg aaagcaattg agggcctatt tcccatgatt ccttcatatt
     2401 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
     2461 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
     2521 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
     2581 tcttggcttt atatatcttg tggaaaggac gaaacaccgg tacctaggct gttggagtct
     2641 tcgggcgtgc gcgtgctggc agccacaacc cgaactatgt tgtcgtggcg tagtcttgca
     2701 atgttcagtt cagcc
//
