LOCUS       sequence-233977-       10197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Cre recombinase in Tribolium castaneum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Gilles AF, Schinko JB, Schacht MI, Enjolras C, Averof M
  TITLE     Clonal analysis by tunable CRISPR-mediated excision.
  JOURNAL   Development. 2019 Jan 4;146(1). pii: dev.170969. doi: 
            10.1242/dev.170969.
  PUBMED    30552128
REFERENCE   2  (bases 1 to 10197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2019-01-4"; volume: "146(1). pii: dev.170969.
            doi"; pages: " 10.1242/dev.170969"
COMMENT     Sequence Label: pBac(3xP3-gTc'v; Tc-hsp68:Cre)
FEATURES             Location/Qualifiers
     source          1..10197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2796..2917
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2833..2852)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2887..2906
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3320..4351)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     repeat_region   6600..6634
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6815..6831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6815..6831)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6828..6850)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6839..6855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6863..6869,6870..6887,6888..6893))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6908..6929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7046..7063)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7217..7805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7297..7316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7976..8767,8768..8836))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8599..8618
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8837..8941)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9009..9027
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9065..9087)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9187..9206
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9400..9422
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9414..9431
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9415..9431
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   10135..10197
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
       61 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
      121 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
      181 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
      241 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
      301 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
      361 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
      421 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
      481 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
      541 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
      601 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
      661 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
      721 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
      781 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
      841 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
      901 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
      961 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccggggatct aattcaatta
     1021 gagactaatt caattagagc taattcaatt aggatccaag cttatcgatt tcgaaccctc
     1081 gaccgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa ttcaaacaag
     1141 caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc tgaacaagct
     1201 aaacaatcgg ggtaccgcta gagtcgacgg taccgcggtc gtaatgagtt gcccactgag
     1261 accctcgtaa gtatgattta atttaattat tccatttaca attaatcgta aattaagtga
     1321 agcccaagaa ggcgaccaac tgagcgaaga atgtgggatg ctttacggtg aatacttgat
     1381 gttggacaag attttagaag cccaaagact actcagcgag caaaataacc aacccgtgca
     1441 cgacgagcac ttatttattg tgacccatca aggtaaaaaa cccacttaac cgtaattaac
     1501 tataacaagt aattccagcg tatgagttat ggttcaaaca aataatctac gagctggact
     1561 cgatccgcaa cattttcagc gacgttttag aagagtcgca aactttggaa atcctcaaac
     1621 gcctcaatcg tgttgttttg atcctcaagg ttcacaattt cgcaacaaat tgcggtcacg
     1681 agataaaact gtcgcaattg taggttttgg tggaccaagt aatgattctg gaaacgatga
     1741 caccgctcga tttcatggac ttccggtgtt atctgcggcc cgcttccggg ttccagagcc
     1801 tgcagttcag gctcctggaa aataagctcg gggtgcgcca ggaaaaccgc gtcaaataca
     1861 accagaatta ctcgaaagtg ttcggaaacg acgaaaaagc gctcgagcaa atcgccaagt
     1921 cggagaaaga gccgtctttg accgatttgg tccagcggtg gctggagcga acgccggggc
     1981 tggagctcga ggggtttaat ttctggggga agtaccaaaa agcggtcgag aagcttttaa
     2041 ccgagcaaaa agaactggcg gagaaagagg aggcggagac tttaaagcgt tacaaattga
     2101 acgatttgga gaagagacgc gaggtttacg agtcgatttt taaagttgag gtccatgagg
     2161 ctctaatgtc gcgcggcgag aggcgcttct cacacaaagc cctgcaaggg gctatcatga
     2221 tcacctttta cagggacgaa cccaggttca gtcagcccca ccaaattcta acacttttga
     2281 tggacataga ctcgctgatt acgaaatggc ggtgttagta aatccgtacg tcaattgttt
     2341 ttgtttaaat tttataattt cagataatca tgttttgatg gtgcaaagaa tgattggttc
     2401 ttcgcagtta ggaacagggg gctcttcggg gtatcagtat ttaaggtcaa cgttaaggtg
     2461 aaattgattt caacacggtt tttgcaatgt ttgattttgt ttttcagcga tcggtataaa
     2521 gttttcgtgg accttttcaa tttatcgacg tttttgatac cacggtccta catccccccc
     2581 ttgtctacgt ctatgagaag tcatttgtgc aattgggggt cggcgaatag caccaataac
     2641 gtgtcgaatg gaaacaatta attatttcgt taaggcggcc gcgactctag atcataatca
     2701 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2761 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     2821 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2881 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaaag cttatcgata cgcgtacggc
     2941 gcgccaagct taaggtgcac ggcccacgtg gccactagtg agcagccagt tgtttttttc
     3001 tatattcctg atgggcatac gaaggagagc ccgtttgcgc ttaatactgc caaggccacg
     3061 actaatgcgg taagtttttt attattttaa aatgaaacaa ctttttatct ttaaattaaa
     3121 taatttatta aaaattttta aataatctac aaaaatcagt ctttaaactt aatgcagtca
     3181 ctatctactt tattataact aaagtaactt tacgtactat tatttacaaa aataaactat
     3241 cacaattaac taagtaggaa cacaaaccga tcatctcgag ctctgtacat gtccgcggtc
     3301 gcgacgtacg cgttaatggc taatcgccat cttccagcag gcgcaccatt gcccctgttt
     3361 cactatccag gttacggata tagttcatga caatatttac attggtccag ccaccagctt
     3421 gcatgatctc cggtattgaa actccagcgc gggccatatc tcgcgcggct ccgacacggg
     3481 cactgtgtcc agaccaggcc aggtatctct gaccagagtc atccttagcg ccgtaaatca
     3541 atcgatgagt tgcttcaaaa atcccttcca gggcgcgagt tgatagctgg ctggtggcag
     3601 atggcgcggc aacaccattt tttctgaccc ggcaaaacag gtagttattc ggatcatcag
     3661 ctacaccaga gacggaaatc catcgctcga ccagtttagt tacccccagg ctaagtgcct
     3721 tctctacacc tgcggtgcta accagcgttt tcgttctgcc aatatggatt aacattctcc
     3781 caccgtcagt acgtgagata tctttaaccc tgatcctggc aatttcggct atacgtaaca
     3841 gggtgttata agcaatcccc agaaatgcca gattacgtat atcctggcag cgatcgctat
     3901 tttccatgag tgaacgaacc tggtcgaaat cagtgcgttc gaacgctaga gcctgttttg
     3961 cacgttcacc ggcatcaacg ttttcttttc ggatccgccg cataaccagt gaaacagcat
     4021 tgctgtcact tggtcgtggc agcccggacc gacgatgaag catgtttagc tggcccaaat
     4081 gttgctggat agtttttact gccagaccgc gcgcctgaag atatagaaga taatcgcgaa
     4141 catcttcagg ttctgcggga aaccatttcc ggttattcaa cttgcaccat gccgcccacg
     4201 accggcaaac ggacagaagc attttccagg tatgctcaga aaacgcctgg cgatccctga
     4261 acatgtccat caggttcttg cgaacctcat cactcgttgc atcgaccggt aatgcaggca
     4321 aattttggtg tacggtcagt aaattggaca tttcacactc agataatggt tttaagtaaa
     4381 gtgtacagga tcggctctgc ccctcgaggg gatcctctag atacactttt cgatttactt
     4441 tgaattcact agtaaataat tcactcaact ttgttaaagt cgctttgaaa atttgctttg
     4501 agtcgcttgc ttagctttgt tgctttcgct tcgcttgatt caaattcact gacaacgcgc
     4561 cgcgagaccg cgcttatata tgaaacgggg aattcgtctc gaacttcacc gaatgatctc
     4621 gaagtttcca ttgatttgcg tttgaatttt ctcgaatttt tatttttaaa acggcctttt
     4681 cggtattatt catgctattt ccgtaaagat aaaggagaat agtggaaact ggttgtgccc
     4741 taatttattt caacttccaa aataaaaaca atcattatat attaatgcat ttaaaaaaaa
     4801 aacgttacat aacgaatgag ttgttgacta tttcatgttt atttttaatt tggtaaagtt
     4861 cggcaatatt atgaatgagt agtgaattgt tttgatttca tatacatatg ccaaagttgg
     4921 attgtcatag caattttaat aaaacaattt tcctattttt tttcttttta aacttattaa
     4981 taggtcttta agtaaaatgt tttagattaa tccttttttt tacttttatc gtttataatc
     5041 aattactttt gcagaagatg cctaaataat agtgattaat aataaataaa gaacaatttt
     5101 ttattgaagg ttgggatcca tatatagggc ccgggttata attacctcag gtcgacgtcc
     5161 catggccatt cgaattcggc cggcctaggc gcgcctaggc cggccgatct cgaacttata
     5221 cggttcttgt agtaagtttt ttgccaaagg gattgaggtg aaccaattgt cacacgtaat
     5281 attacgacaa ctaccgtgca caggctttga taactccttc acgtagtatt caccgagtgg
     5341 tactccgttg gtctgtgttc ctcttcccaa ataaggcatt ccatttatca tatacttcgt
     5401 accactgtca cacatcatga ggatttttat tccatactta cttggcttgt ttgggatata
     5461 catcctaaac ggacaccgtc ctctaaaacc aagtaactgt tcatctatgg tcaaatgagc
     5521 ccctggagtg taattttgta tgcactgatg gataaagaga tcccatattt ttctaacagg
     5581 agtaaataca tcgttttctc gaagtgtggg ccgtatactt ttgtcatcca ttctaagaca
     5641 tcgtatcaaa aaatcaaaac gatcacgact cattacagag acgtacacca ttgacaaaga
     5701 tcgatcaaag aggtcatctg tggacatgtg gttatctttt ctcactgctg tcattaccag
     5761 aataccaaag aaagcataga tttcatcttc attcgtgtca cgaaatgtag cacctgtcat
     5821 agattcccga cgtttcaatg atatctcagc atttgtccat tttacaattt ccgaaattat
     5881 ctcatcagta aaaaatagtt tgaagcataa aagtgggtca tatatattgc ggcacatacg
     5941 cgtcggacct ctttgagatc tgacaatgtt cagtgcagag actcggctac gcctcgtgga
     6001 ctttgaagtt gaccaacaat gtttattctt acctctaata gtcctctgtg gcaaggtcaa
     6061 gattctgtta gaagccaatg aagaacctgg ttgttcaata acattttgtt cgtctaatat
     6121 ttcactaccg cttgacgttg gctgcacttc atgtacctca tctataaacg cttcttctgt
     6181 atcgctctgg acgtcatctt cacttacgtg atctgatatt tcactgtcag aatcctcacc
     6241 aacaagctcg tcatcgcttt gcagaagagc agagaggata tgctcatcgt ctaaagaact
     6301 acccatttta ttatatatta gtcacgatat ctataacaag aaaatatata tataataagt
     6361 tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag
     6421 tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag
     6481 tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc
     6541 ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc
     6601 atgcgtcaat tttacgcaga ctatctttct agggttaaaa aagatttgcg ctttactcga
     6661 cctaaacttt aaacacgtca tagaatcttc gtttgacaaa aaccacattg tggccaagct
     6721 gtgtgacgcg acgcgcgcta aagaatggca aaccaagtcg cgcgagcgtc gactctagag
     6781 gatccccggg taccgagctc gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt
     6841 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6901 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6961 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7021 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7081 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7141 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7201 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7381 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7501 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7561 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7621 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7681 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7741 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7801 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7861 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7921 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7981 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8041 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8101 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8161 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8221 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8281 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8341 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8401 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8461 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8521 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8581 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8641 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8701 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8761 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8821 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8881 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8941 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     9001 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     9061 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     9121 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     9181 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     9241 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     9301 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     9361 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     9421 cgacggccag tgccaagctt tgtttaaaaa tataacaaaa ttgtgatccc acaaaatgaa
     9481 gtggggcaaa atcaaataat taatagtgtc cgtaaacttg ttggtcttca actttttgag
     9541 gaacacgttg gacggcaaat ccgtgactat aacacaagtt gatttaataa ttttagccaa
     9601 cacgtcgggc tgcgtgtttt ttgccgacgc gtctgtgtac acgttgatta actggtcgat
     9661 taaactgttg aaataattta atttttggtt cttctttaaa tctgtgatga aattttttaa
     9721 aataacttta aattcttcat tggtaaaaaa tgccacgttt tgcaacttgt gagggtctaa
     9781 tatgaggtca aactcagtag gagttttatc caaaaaagaa aacatgatta cgtctgtaca
     9841 cgaacgcgta ttaacgcaga gtgcaaagta taagagggtt aaaaaatata ttttacgcac
     9901 catatacgca tcgggttgat atcgttaata tggatcaatt tgaacagttg attaacgtgt
     9961 ctctgctcaa gtctttgatc aaaacgcaaa tcgacgaaaa tgtgtcggac aatatcaagt
    10021 cgatgagcga aaaactaaaa aggctagaat acgacaatct cacagacagc gttgagatat
    10081 acggtattca cgacagcagg ctgaataata aaaaaattag aaactattat ttaaccctag
    10141 aaagataatc atattgtgac gtacgttaaa gataatcatg cgtaaaattg acgcatg
//
