LOCUS       sequence-233980-        2674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ssRNA expression for dsRNA production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2674)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U6-Lin28a-s
FEATURES             Location/Qualifiers
     source          1..2674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        68..316
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     68..88
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     239..258
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(661..679)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        747..851
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(852..920,921..1712)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1070..1089)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1883..2471
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2372..2391
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2625..2642
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
       61 agcaattgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
      121 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
      181 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
      241 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
      301 gaaaggacga aacaccgcgg ccgcggagtt taaggaaaga ggcatcaatc tgcagagtgg
      361 agaaagtggg ggtaagggtg ggttgcgtgg gtagcttgca ctgccgtgtc tcaggccggg
      421 gttcccagtg tcaccctgtc tttccttgga gggaaggaaa ggatgagaca aaggaactcc
      481 taccacactc tatctgaaag caagtgaagg cttttgtggg gaggaaccac cctagaaccc
      541 gaggctttgc caagtggctg ggctagggaa gttcttttgt agaaggctgt gtgatatttc
      601 ccttgccaga cgggaagcga aacaagtgtc aaaccgcggc cgcttttttc tgcaggggcg
      661 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      721 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      781 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      841 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      901 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      961 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1021 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1081 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1141 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1201 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1261 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1321 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1381 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1441 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1501 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1561 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1621 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1681 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1741 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1801 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1861 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1921 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1981 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2041 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2101 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2161 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2221 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2281 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2341 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2401 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2461 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2521 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2581 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2641 aggaagcgga agagcgccca atacgcaaac cgcc
//
