LOCUS       sequence-233987-        2674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ssRNA expression for dsRNA production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2674)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U6Lin28a-as
FEATURES             Location/Qualifiers
     source          1..2674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..328
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     80..100
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     251..270
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(673..691)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        759..863
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(864..932,933..1724)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1082..1101)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1895..2483
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2384..2403
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2637..2654
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
       61 tcccgactgg aaagcaattg agggcctatt tcccatgatt ccttcatatt tgcatatacg
      121 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
      181 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
      241 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
      301 atatatcttg tggaaaggac gaaacaccgc ggccgcggtt tgacacttgt ttcgcttccc
      361 gtctggcaag ggaaatatca cacagccttc tacaaaagaa cttccctagc ccagccactt
      421 ggcaaagcct cgggttctag ggtggttcct ccccacaaaa gccttcactt gctttcagat
      481 agagtgtggt aggagttcct ttgtctcatc ctttccttcc ctccaaggaa agacagggtg
      541 acactgggaa ccccggcctg agacacggca gtgcaagcta cccacgcaac ccacccttac
      601 ccccactttc tccactctgc agattgatgc ctctttcctt aaactccgcg gccgcttttt
      661 tctgcagggg cggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      721 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      781 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      841 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      901 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      961 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1021 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1081 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     1141 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1201 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1261 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1321 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1381 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     1441 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1501 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1561 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     1621 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1681 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1741 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1801 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1861 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1921 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1981 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2041 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2101 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2161 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2221 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2281 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2341 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2401 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2461 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2521 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2581 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2641 agtcagtgag cgaggaagcg gaagagcgcc caat
//
