LOCUS       sequence-233989-        4216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Renilla luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4216)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RL-Elavl2
FEATURES             Location/Qualifiers
     source          1..4216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        78..435
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        78..269
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(101..121)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      286..421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          505..637
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     620..639
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        682..700
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      704..713
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             710..1645
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    2187..2308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2198..2217)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2252..2271
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2441..2545
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2546..2614,2615..3406)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2764..2783)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3577..4165
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4066..4085
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcacatgg ctcgacagat ctgcgcagca ccatggcctg aaataacctc tgaaagagga
       61 acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
      121 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      181 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      241 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
      301 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
      361 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      421 ctaggctttt gcaaaaagct tgattcttct gacacaacag tctcgaactt aagctgcaga
      481 agttggtcgt gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac
      541 caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt
      601 ggtcttactg acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac
      661 agctcttaag gctagagtac ttaatacgac tcactatagg ctagccacca tggcttccaa
      721 ggtgtacgac cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg
      781 caagcaaatg aacgtgctgg actccttcat caactactat gattccgaga agcacgccga
      841 gaacgccgtg atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt
      901 gcctcacatc gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc
      961 cggcaagagc gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg
     1021 gttcgagctg ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg
     1081 tctggccttt cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga
     1141 gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc
     1201 cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac
     1261 catgctccca agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga
     1321 gccattcaag gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc
     1381 tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct
     1441 tcgggccagc gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa
     1501 cgctattgtc gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct
     1561 ccacttcagc caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga
     1621 gcgcgtgctg aagaacgagc agtaattcta gaaaacccta caccccttga tatgccagtg
     1681 acctggtcga caagaatccg agaagtaatg atgcgcccat actgtgaaaa cagctgttcc
     1741 agctccttct gggtcatggt ttttggaagc ccgctgacgt ataagtttgc atctctgatt
     1801 gaggctgagc ttgggcgagc ataggaaact tttgttgttt tggtttgaag tctcaatcca
     1861 ttcagagtgt tgatagcttt ctctgcatcc ttggggtcaa tgtagttcac aaatccgtaa
     1921 cccaagctct gccctgttat tttgtctctt acaagcttac aggactctat ctcgccaatg
     1981 ctcccaaaaa gactctttag ttcttcctgt gtcatgttct gaggaaggta gttgactatt
     2041 aggttggtct tgctgtcctc tgtgttcccg gagtcaactg gtgaggagca gttgttgttt
     2101 acagtggttg gaccattggc tgtgttattg caagttggcc cattagacag ttgtgtttct
     2161 agagcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     2221 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2281 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2341 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     2401 aatcgataag gatccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2461 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2521 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2581 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2641 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2701 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2761 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2821 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2881 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2941 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3001 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3061 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3121 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3181 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3241 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3301 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3361 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3421 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3481 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3541 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3601 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3661 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3721 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3781 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3841 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3901 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3961 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4021 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4081 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4141 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4201 cttttgctgg cctttt
//
