LOCUS       sequence-233990-        5995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses truncated variant (aa271-aa500) of human FOXP2 in 
            bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Haussermann K, Young G, Kukura P, Dietz H
  TITLE     Dissecting FOXP2 Oligomerization and DNA Binding.
  JOURNAL   Angew Chem Int Ed Engl. 2019 Jun 3;58(23):7662-7667. doi: 
            10.1002/anie.201901734. Epub 2019 Apr 29.
  PUBMED    30887622
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/anie.201901734"; journalName: "Angew Chem Int Ed Engl"; 
            date: "2019-06-3- 3"; volume: "58"; issue: "23"; pages: "7662-7667"
COMMENT     Sequence Label: pET28b-FOXP2_P6
FEATURES             Location/Qualifiers
     source          1..5995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(53..71)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      67..114
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      151..606
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(238..257)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     448..469
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(699..1514)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(699..1514)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1422..1441
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1506..1525
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1636..2224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2125..2144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2378..2395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2410..2552
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2478..2497)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2638..2660
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2654..2845)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3654..4736)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4698..4717
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4737..4814)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5020..5039
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5123..5142
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5123..5141
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5142..5166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5181..5203
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5222..5239
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5249..5266
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5969..5992
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
       61 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
      121 ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
      181 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      241 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      301 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      361 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
      421 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
      481 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
      541 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
      601 caatttcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      661 aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc
      721 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      781 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      841 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      901 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      961 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     1021 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     1081 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     1141 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     1201 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     1261 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     1321 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     1381 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     1441 ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt
     1501 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1561 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1621 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1681 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1741 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1801 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1861 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1921 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1981 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2041 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2101 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2161 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2221 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2281 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2341 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2401 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     2461 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     2521 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     2581 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     2641 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     2701 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
     2761 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     2821 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     2881 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     2941 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
     3001 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
     3061 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
     3121 tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg
     3181 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     3241 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca
     3301 tgcgcacccg tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg
     3361 tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg
     3421 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg
     3481 ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga
     3541 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     3601 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc
     3661 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     3721 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     3781 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     3841 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     3901 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     3961 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     4021 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
     4081 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
     4141 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     4201 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
     4261 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
     4321 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     4381 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
     4441 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     4501 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     4561 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     4621 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
     4681 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
     4741 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc
     4801 cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca
     4861 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
     4921 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
     4981 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
     5041 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
     5101 cgagatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac
     5161 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacca tgggcagcag
     5221 ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata tgaaagaagt
     5281 gactggagtt cacagtatgg aagacaatgg cattaaacat ggagggctag acctcactac
     5341 taacaattcc tcctcgacta cctcctccaa cacttccaaa gcatcaccac caataactca
     5401 tcattccata gtgaatggac agtcttcagt tctaagtgca agacgagaca gctcgtcaca
     5461 tgaggagact ggggcctctc acactctcta tggccatgga gtttgcaaat ggccaggctg
     5521 tgaaagcatt tgtgaagatt ttggacagtt tttaaagcac cttaacaatg aacacgcatt
     5581 ggatgaccga agcactgctc agtgtcgagt gcaaatgcag gtggtgcaac agttagaaat
     5641 acagctttct aaagaacgcg aacgtcttca agcaatgatg acccacttgc acatgcgacc
     5701 ctcagagccc aaaccatctc ccaaacctct aaatctggtg tctagtgtca ccatgtcgaa
     5761 gaatatgttg gagacatccc cacagagctt acctcaaacc cctaccacac caacggcccc
     5821 agtcaccccg attacccagg gaccctcagt aatcacccca gccagtgtgc ccaatgtggg
     5881 agccatacga aggcgacatt cagacaaata caacattccc atgtcatcag aaattgcccc
     5941 aaactatgaa ttttataaaa atagcgcttg gagccacccg cagttcgaaa aataa
//
