LOCUS       sequence-233992-        5403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of full length ELK1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Zhou J, Hu G, Herring BP
  TITLE     Smooth muscle-specific genes are differentially sensitive to 
            inhibition by Elk-1.
  JOURNAL   Mol Cell Biol. 2005 Nov . 25(22):9874-85.
  PUBMED    16260603
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2005 Nov . 25(22):9874-85."
COMMENT     Sequence Label: Elk1 pShuttle
FEATURES             Location/Qualifiers
     source          1..5403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        128..507
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        508..711
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     662..682
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     756..775
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        756..774
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             797..823
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2158..2175)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2164..2388
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2553..2570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2724..3312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2804..2823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4009..4824)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4009..4824)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4732..4751
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4816..4835
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(5340..5359)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
       61 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
      121 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      181 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      241 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      301 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      361 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      421 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      481 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      541 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      601 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      661 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
      721 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
      781 caagctggct agcatgtacc catacgatgt tccagattac gctgggccct ctagaatgga
      841 cccatctgtg acgctgtggc agtttctgct gcagcttctg agagaacaag gtaatggcca
      901 catcatctcc tggacctcac gggatggtgg tgagttcaag ttggtggatg cagaggaggt
      961 ggcccggcta tggggactgc gcaagaacaa gaccaacatg aattacgaca agcttagccg
     1021 ggccttgcgg tactactatg ataagaatat catccgcaag gtgagcggcc agaagtttgt
     1081 ctacaagttt gtgtcctacc cagaggttgc agggtgctcc actgaagact gcccacccca
     1141 gcctgaggtg tctgtaacct cggccatagc catggcccct gctactgtcc atgcaggccc
     1201 aggggacact gccactggaa agccaggaac accaaagggt gcaggaatga caggccaagg
     1261 tggcttagca cgaagcagcc ggaatgaata catgcgctcg ggcctctatt ctaccttcac
     1321 aatacaatcc ctgcagccac agccacagcc acccattcct cctcggcctg cctcagtgct
     1381 tcccaacact acccctgcag gagtaccagc acccgcctca gggagcagga gcaccagtcc
     1441 aaacccctta gaagcctgtt tggaagcaga agaggctggt ctgcccctgc aggttatcct
     1501 aaccccacca gaggccccaa accagaaatc cgaagagttg agtctggacc caagttttgg
     1561 ccatccacag cccccagaag tcaaagtgga ggggcctaag gaagaattgg aagctgcaag
     1621 ggctggaggc ttcagttcag aagctgtcaa agctgaacca gaagtctcag cctcagaagg
     1681 cctgctggct cggctcccag ccatcctaac agagaacaca gcccaggtgt gtggcctctc
     1741 cacttccacc actgagatca cccaaccgca gaagggccga aagcctcggg acctggaact
     1801 tccacttagc ccaagcctgc tgggtggcca gggacctgaa cggactccag gatcaggaac
     1861 aagctctggt cttcaggcac cggggccagc gctaacgcca tccctgctcc ccacacatac
     1921 cttgaccccg gtgctgctga cacccagctc gctgccccct agcatccatt tctggagcac
     1981 tctgagtcca attgcacccc gtagtccagc caagctctcc ttccagtttc cgtccagtgg
     2041 cagcgcacag gtgcacatcc cttccatcag tgtggatggc ctctcgaccc ccgtggtgct
     2101 ctccccaggg ccccagaagc catgaggtac caagcttaag tttaaaccgc tgatcagcct
     2161 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     2221 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     2281 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     2341 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     2401 aaagaaccag cagatctgca gatctgaatt catctatgtc gggtgcggag aaagaggtaa
     2461 tgaaatggca ttatgggtat tatgggtctg cattaatgaa tcggccaacg cgcggggaga
     2521 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2581 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2641 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2701 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2761 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2821 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2881 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2941 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3001 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3061 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3121 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3181 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3241 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3301 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3361 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3421 ttgatcctcc ggcgttcagc ctgtgccaca gccgacagga tggtgaccac catttgcccc
     3481 atatcaccgt cggtactgat cccgtcgtca ataaaccgaa ccgctacacc ctgagcatca
     3541 aactctttta tcagttggat catgtcggcg gtgtcgcggc caagacggtc gagcttcttc
     3601 accagaatga catcaccttc ctccaccttc atcctcagca aatccagccc ttcccgatct
     3661 gttgaactgc cggatgcctt gtcggtaaag atgcggttag cttttacccc tgcatctttg
     3721 agcgctgagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc
     3781 catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc
     3841 agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg
     3901 tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca
     3961 agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc
     4021 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     4081 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     4141 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     4201 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     4261 gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     4321 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     4381 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     4441 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     4501 ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     4561 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     4621 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     4681 atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     4741 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga
     4801 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     4861 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
     4921 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca
     4981 tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc
     5041 cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc
     5101 gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgctagat
     5161 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5221 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtaact
     5281 ataacggtcc taaggtagcg aaagctcaga tctcccgatc ccctatggtg cactctcagt
     5341 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     5401 gtc
//
