LOCUS       sequence-233998-        7435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Fulco CP, Munschauer M, Anyoha R, Munson G, Grossman SR, Perez EM, 
            Kane M, Cleary B, Lander ES, Engreitz JM
  TITLE     Systematic mapping of functional enhancer-promoter connections with 
            CRISPR interference.
  JOURNAL   Science. 2016 Sep 29. pii: aag2445.
  PUBMED    27708057
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Sep 29. pii: aag2445."
COMMENT     Sequence Label: pGL4.23-MYC-e4
FEATURES             Location/Qualifiers
     source          1..7435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    80..201
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2494..2513
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    3052..3100
                     /note="synthetic polyadenylation signal"
     misc_feature    3114..3205
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3154..3173
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      3479..3488
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3485..5137
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3551..3569)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    5178..5299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5547..5564)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5718..6306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5798..5817)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6506..7297,7298..7366))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accccagtaa atgcttttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
       61 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
      121 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
      181 caaactcatc aatgtatctt atcatgtctg tggaaaacgg gacagggaca tggtcagaac
      241 tgtgctttca gaatttggac ttggccactg gaagaagaga cagttaaact caagttgccc
      301 aacatgaggc atgggatggg agaggttggg gaagggatgg aaggaaacag agtgggcacc
      361 aacaggaagg aagcaaagtc aggaactgct ggctggaatg ggtccaggga gagggaaaac
      421 cacttctcct tcctcttctt atgattgcaa gattagtact tgagaattag gctgcacagg
      481 ccaggacttc ccaaaactca ggagggaaag agcccagagc acgtgtctcc aatggaggct
      541 atagttccaa atcacgtgat catcggtcat ctccttgccc accctgaagc ccttgtcccc
      601 agtgtacttt gggctcagac agtccctcag gggatacagc agtgtccttt ccttcagcat
      661 tcagggcaaa tgagaggcaa agagatcaat ggaatgcaaa agcgttacta tagatgagaa
      721 gtcggacgca cagagaggtt ccaccacttg cctaatatca cacagcaagt acatgccaga
      781 atcaaaattc aaacctagag ccccggtgta aaccttacct gaatcagtca gggagcagca
      841 gggaggcagt ggaaggatag gccccctgac ctcactcccc gcctgccttc ccatcctgcc
      901 agtgccttcc aaactgatac cagagagcat ggcagccctg tgttgcatcc accctgataa
      961 tgcatccaca caggtcagcc ttctgggcac aaaacaaggc agagaagagc aaggagtgga
     1021 cctggagggg cccacggaag ctccccagca cccaccactc caatggccca gcccctggta
     1081 ggtctccagt gcccttcacc ctacagagcc acagaatccc cagaccttga cgctgcagct
     1141 agggcccgcc tgcctctcaa gagaagggca gggccccaca caggcaagtg ctctgcctcc
     1201 gcgcaaagct gggcatgcgt catcacactc ccgccttcct tcctcattca ctctcccttg
     1261 ccctgcttct atcagtggct ccaagacgca aacatctcga aatgacctgc ttctgtgtag
     1321 gtcagctaac agaagtgctg tcttaagaaa tgagggccag ctgatgccca ctaccagacc
     1381 cagccagccc ctcccagcct ccctgccagc cctgcacatg gaggcctggc ggccaggaaa
     1441 aagaaagggc tcctatctct gattcctcag ggcctctgat aagaaatgtc atcccttgct
     1501 gggtaatatt accaagccag ataaaaatct ctatcaagct taaacatatg gctggctgca
     1561 gaacacagag tccagtggag aagggtaatt tatttgctta agctgaaaac cctgggcagc
     1621 cgaaagaagt gcctgaataa aagagtaaga gccaaattat aacctcttct gacttggcat
     1681 ggggattttt ccctgggaag gatgagacac ctgagaacaa cagggggctc tccacacatt
     1741 tctcctgaca caataagcgc tttcgccttc ctctttcttc aaagggcact ttcacttatg
     1801 ctatcacatt taatcctcac tacaacctca agagaggcat ttttagcttc ctttacagat
     1861 caggaaattg agattcagca aggttaagta aagtggccaa ggtcacacag gcagccatgc
     1921 tggagcgagg acccaaaccc cagcaggtct gactcaccat cagatgggga agctgatccc
     1981 agcttctggg gaggatcggc tcatacatcc acttaatcag cgaagtcaga tctgcccaac
     2041 ttaactgaca atgtcagcta cacattgtaa tcttgaaggt ccatttcaag gctctcccgt
     2101 ctccttgttt ctctgtagcc taaaggataa tgagaaagcc ttgcagagca cagaagctca
     2161 aggaaaagga cgggaggcca gggatagtac ccaggagagc agtgagtgac ctcagggctt
     2221 agattccagc ctcatatgct caaggagtag caggtagaag gaaactgtgg aaagaaaact
     2281 cctctctctg ttcattcatt cattcacaaa tattagtgga aagcctattg tgtgccgcaa
     2341 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg
     2401 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     2461 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     2521 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     2581 catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa
     2641 gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat
     2701 tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg
     2761 caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg
     2821 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt
     2881 tagcagccct tgcgccctga attttgttaa aattcgcgtt aaatttttgt taaatcagct
     2941 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gagtacgtct
     3001 ctcaaggata agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata
     3061 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac
     3121 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca
     3181 agtgcaggtg ccagaacatt tctctggcct aactggccgg tacctgagct cgctagcctc
     3241 gaggatatca agatctggcc tcggcggcca agcttacccc cgagctgtgc tgctcgcggc
     3301 cgccaccgcc gggccccggc cgtccctggc tcccctcctg cctcgagaag ggcagggctt
     3361 ctcagaggct tggcgggaaa aagaacggag ggagggatcg cgctgagtat aaaagccggt
     3421 tttcggggct ttatctaact cgctgtagta attccggcaa tccggtactg ttggtaaagc
     3481 caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga
     3541 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
     3601 aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat
     3661 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
     3721 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
     3781 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg
     3841 catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
     3901 gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca
     3961 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
     4021 cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc
     4081 tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt
     4141 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
     4201 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
     4261 atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga
     4321 ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac
     4381 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct
     4441 ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg
     4501 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc
     4561 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg
     4621 gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc
     4681 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca
     4741 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa
     4801 gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct
     4861 ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga
     4921 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt
     4981 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt
     5041 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct
     5101 cataaaggcc aagaagggcg gaaagatcgc cgtgtaattc tagagtcggg gcggccggcc
     5161 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     5221 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     5281 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     5341 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
     5401 ggatccgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     5461 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     5521 aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5581 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5641 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5701 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5761 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5821 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5881 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5941 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6001 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6061 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6121 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6181 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6241 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6301 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6361 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6421 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc
     6481 cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag
     6541 cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga
     6601 ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac
     6661 cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc
     6721 ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa
     6781 gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac
     6841 gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat
     6901 gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa
     6961 gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg
     7021 tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg
     7081 agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc
     7141 cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact
     7201 caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat
     7261 cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg
     7321 ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc
     7381 aatattattg aagcatttat cagggttaca ctgcggctcc tcatcgcgat aataa
//
