LOCUS       sequence-233999-        7262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Fulco CP, Munschauer M, Anyoha R, Munson G, Grossman SR, Perez EM, 
            Kane M, Cleary B, Lander ES, Engreitz JM
  TITLE     Systematic mapping of functional enhancer-promoter connections with 
            CRISPR interference.
  JOURNAL   Science. 2016 Sep 29. pii: aag2445.
  PUBMED    27708057
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Sep 29. pii: aag2445."
COMMENT     Sequence Label: pGL4.23-MYC-e5
FEATURES             Location/Qualifiers
     source          1..7262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    55..176
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2296..2315
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    2854..2902
                     /note="synthetic polyadenylation signal"
     misc_feature    2916..3007
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2956..2975
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      3281..3290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3287..4939
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3353..3371)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    4980..5101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5349..5366)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5520..6108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5600..5619)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6308..7099,7100..7168))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
       61 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      121 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
      181 gtctgtggaa aacgagtggg gacatgattc caaatacatg ggctgccttc aaatcctggc
      241 ccacaagcct acatccttac aacagaaccc tctctggaaa tgagacaatt atttctttct
      301 agtgggggtt gcagagagta gaggaagaga gttggaaagg agaaggagag ctagtggatg
      361 ggaaaccaac aaaatgtggt tcattttcag gaagctgcga ggctggatct ttcagaacag
      421 ctcatatttt ttcgagatgc attttttttg aggtccccca ctccggatac cccagtgccc
      481 tcctttcagc cttggccctt gcatcaccac cagccagggg aaggagccgt ggcatggcag
      541 tggtcacagt tctcttggat aagctttacc gtaatcttca taaacctcaa tgctcaaggt
      601 gcagagggag attggaggaa acttgttttt ccgttggttt ctctctgact gctgcggcta
      661 atcattccta gaacaggaag ctggagaaat gtaggtagga gacactcttc tgacaagtca
      721 ggttgcctca cccttccagg gcacaactga ttaggaaaag cttcagggtt acataacctc
      781 cttcctttac ggtggttttc catcaaaagg tgaatggact cagccctgag aactgcccac
      841 ccctaagaag cggggaaggg atgtcttggt tgtattttag tattgctgcc tccttcatca
      901 caatgcccca gtgcacaagt aaggggagga aaataggatg cattaattta ttccatgaac
      961 ctttattgag tgtccattct tctatgtatt tgaaatacat cagtaaagga aatgaactct
     1021 accctggtac actcatatcc tagtggaggg acacagagta aaccataata cataagtaat
     1081 ttacattgtg ggttagaagt tgatgaatga aatcaagaaa agtagagcag ggacagaggg
     1141 ataggtggtt gggagggcaa aggcatggat tgcaattttt aacggagcac aaggtaagac
     1201 tccttgaaaa gatgacattt gagcaaagac ttgaaagaga tgaaggtgtg agctacatga
     1261 ctatatgaag aaatagcaaa ggcctaaggc aggagtgttt ccagagttgt tgtcagggtg
     1321 ggaggagcac agtgagcaag gaagaaaaaa gtaaaagatg atgtatgtga ttggctgggg
     1381 ggcaggtgtc agatcaggtg gcactttgca agtgattgtt aaggactctg tgcttttaga
     1441 gtaaataaaa tggcagctat tatcgttctg agaagagtaa cagaaccagc acgttcaccc
     1501 tggtgctcct tctggggtga gagcaaacac agaaagggtc atccgatatc attaacagct
     1561 gtctgtactg gaggcctctt taggcttctg ggtcaagtca aataacctac agacccacct
     1621 atcctcatgg ccggggtggg ggtgggccag aagagtgaca gcctatgtgt ttaacaacat
     1681 gatttaaatc attcaagtga cccactgaga gctgtgaaag caattttgaa tttcttttcc
     1741 tgcagaagga atggctttac cttaagtcac taccttcctc aatcttcttt gtgaccaaaa
     1801 agacagattc caaacactgc cagctgagta caatctacac cctccaaata tagcagagct
     1861 aactgcctac accggaccct ggtgccatta gctcatgctc tagctccctg cactggccac
     1921 ccatactgct ttatgaaatg caatctcttt ttaaagataa tgacacagta agtgtgttat
     1981 gacttcagtg atacagtaag ttaggcaaat tctttgaaag ctatcagctc attttgagca
     2041 gggccaccag cctctggtag agagaagttt gttgatctga agtcataaaa ccaaggggta
     2101 aaggacatgc tgaaccagac gataaacacc gtcatggcgc aaggaacgcc cgtcgtggcc
     2161 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     2221 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2281 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2341 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2401 gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg
     2461 cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc
     2521 cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc
     2581 tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag
     2641 caccgtttct gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct
     2701 gaattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     2761 ccgaaatcgg caaaatccct tataaatcaa aagagtacgt ctctcaagga taagtaagta
     2821 atattaaggt acgggaggta ttggacaggc cgcaataaaa tatctttatt ttcattacat
     2881 ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa
     2941 acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca
     3001 tttctctggc ctaactggcc ggtacctgag ctcgctagcc tcgaggatat caagatctgg
     3061 cctcggcggc caagcttacc cccgagctgt gctgctcgcg gccgccaccg ccgggccccg
     3121 gccgtccctg gctcccctcc tgcctcgaga agggcagggc ttctcagagg cttggcggga
     3181 aaaagaacgg agggagggat cgcgctgagt ataaaagccg gttttcgggg ctttatctaa
     3241 ctcgctgtag taattccggc aatccggtac tgttggtaaa gccaccatgg aagacgccaa
     3301 aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca
     3361 actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc
     3421 acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga
     3481 agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc
     3541 tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc
     3601 gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt
     3661 ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat
     3721 catccaaaaa attattatca tggattctaa aacggattac cagggatttc agtcgatgta
     3781 cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc
     3841 cttcgatagg gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc
     3901 taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc
     3961 tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca
     4021 cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat
     4081 gtatagattt gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc
     4141 gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga
     4201 tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga
     4261 agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac
     4321 tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt
     4381 tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa
     4441 tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc
     4501 ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag acatagctta
     4561 ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa
     4621 aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt
     4681 cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt
     4741 tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca
     4801 agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg
     4861 tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg
     4921 cggaaagatc gccgtgtaat tctagagtcg gggcggccgg ccgcttcgag cagacatgat
     4981 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     5041 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     5101 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     5161 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc
     5221 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     5281 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     5341 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5401 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5461 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5521 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5581 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5641 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5701 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5761 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5821 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5881 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5941 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6001 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6061 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6121 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6181 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6241 tcaatctaaa gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact
     6301 gcagtggtta ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt
     6361 cgtccatagt ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat
     6421 caggccccag cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag
     6481 caatgaacca gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct
     6541 ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt
     6601 tccgaagagt tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg
     6661 cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa
     6721 gaaatgcagt cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt
     6781 tgtcgctcat ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat
     6841 gcttttccgt gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac
     6901 caagctgctc ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga
     6961 aagtgctcat catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat
     7021 tgagatccag ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt
     7081 tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga
     7141 gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt
     7201 atcagggtta cactgcggct cctcatcgcg ataataaacc ccagtaaatg cttttaaaaa
     7261 ac
//
