LOCUS       sequence-234006-        2896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2896)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: H1-Lin28-6U
FEATURES             Location/Qualifiers
     source          1..2896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..333
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     85..105
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     256..275
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(735..754)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(900..918)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        986..1090
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1091..1159,1160..1951)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1309..1328)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2122..2710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2611..2630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2864..2881
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
       61 aggtttcccg actggaaagc aattgagggc ctatttccca tgattccttc atatttgcat
      121 atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata
      181 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
      241 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
      301 gctttatata tcttgtggaa aggacgaaac accgggaaaa agcttggagt ttaaggaaag
      361 aggcatcaat ctgcagagtg gagaaagtgg gggtaagggt gggttgcgtg ggtagcttgc
      421 actgccgtgt ctcaggccgg ggttcccagt gtcaccctgt ctttccttgg agggaaggaa
      481 aggatgagac aaaggaactc ctaccacact ctatctgaaa gcaagtgaag gcttttgtgg
      541 ggaggaacca ccctagaacc cgaggctttg ccaagtggct gggctaggga agttcttttg
      601 tagaaggctg tgtgatattt cccttgccag acgggaagcg aaacaagtgt caaaccgcgg
      661 ccgctttttc cctctatcac tgatagggaa cttataagat tcccaaatcc aaagacattt
      721 cacgtttatg gtgatttccc agaacacata gcgacatgca aatattgcag ggcgccactc
      781 ccctgtccct cacagccatc ttcctgccag ggcgcacgcg cgctgggtgt tcccgcctag
      841 tgacactggg cccgcgattc cttggagcgg gttgatgacg tcagcgttct gcaggggcgg
      901 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      961 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1021 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1081 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1141 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1201 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1261 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1321 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1381 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1441 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1501 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1561 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1621 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1681 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1741 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1801 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     1861 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1921 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1981 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2041 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2101 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2161 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2221 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2281 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2341 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2401 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2461 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2521 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2581 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2641 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2701 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2761 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2821 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2881 ggaagcggaa gagcgc
//
