LOCUS       sequence-234009-        6790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Fulco CP, Munschauer M, Anyoha R, Munson G, Grossman SR, Perez EM, 
            Kane M, Cleary B, Lander ES, Engreitz JM
  TITLE     Systematic mapping of functional enhancer-promoter connections with 
            CRISPR interference.
  JOURNAL   Science. 2016 Sep 29. pii: aag2445.
  PUBMED    27708057
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Sep 29. pii: aag2445."
COMMENT     Sequence Label: pGL4.23-MYC-e3
FEATURES             Location/Qualifiers
     source          1..6790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    75..196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1844..1863
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    2402..2450
                     /note="synthetic polyadenylation signal"
     misc_feature    2464..2555
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2504..2523
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      2829..2838
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2835..4487
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2901..2919)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    4568..4649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4897..4914)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5068..5656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5148..5167)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5856..6647,6648..6716))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agtaaatgct tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
       61 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      121 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      181 tcatcaatgt atcttatcat gtctgtggaa aacgtgtccc cttggtttcc tctttcagca
      241 ttgtcatttc ttctgaaagg tgagcagcta tgaatctgag caagagtgag aaggttcgag
      301 ctaacacttg cttaggaggg ctggaactac atctgaaata gtttggggtc tgtagcttct
      361 tgttagtccc cagaatccct ataacaggga tgctctaaga ctgtggtcct caaactccac
      421 tgcataatag aatcacctgg ggagatgtta taaatccaaa tgcccaggtc ccatttcaga
      481 caaaatcaaa atctttgcag ctggggtact agcagcagga tttaaaaaat cctctaggca
      541 attccaagct tcaacccagt cttagaacaa ctgatctaag agtcaaatcc aacaggaaag
      601 gaaaacatgg cagagaggaa agcatcccca tccttgagca gtgtcatctg cagaactact
      661 tcagctcatt atgaagccgc tcctcattac gccttacagg gcagctcggt tgtcacaagg
      721 agctaacaga cttgaaagtt gccctggatc actgcttttg taacacaccc ttccaaagtc
      781 ttctggaagc aggtaagggt agaaaatacc aacaaataca gccatgtggg taactcttgt
      841 ggaaaaagca ttgggttgcg tgcagacagc tggttctggc cctaggctct ttcactaata
      901 ctacgtaact taaaaagcat ataacactta tgcatcagtt tcttcagtag tgaaataaag
      961 ataagaactg acttacccat tagataagat gttgtggggt acaagccaga agacataggt
     1021 cagctgtggc aaagcatttt tatttaacat gttttccagg tcttcaatgg aacataaaaa
     1081 gtttaggaga gagaaatgtc cccttaaaca ttttttttca atgagagaag ggaggagtca
     1141 aggagtagag ttggtcggcc acatgaaagc tcccttcaga gcaggctgcc gtggtgcctg
     1201 cccacaaggg gtacaagtgg ggccagtgat agggcaggag ctcagggtgt gtccaagggc
     1261 tgccccagca aagacgttaa gataggatat gttatctggg aacaatggaa caactgtacc
     1321 gttgtcctaa acctgaatct gacaagaggg gataagactc caggaagcat gtccccaaac
     1381 cactggccag agtggcccca acaaaccact ggtccatttg ttcattcctt tatctactta
     1441 ctcattcatg taccttacag tagtgtatgt caactacata tcaagaattg tatggatgta
     1501 caagattgta ggaatgctgg aatgaataag gctcactccg caccctcagg agggacaaag
     1561 ataagtaggg caaacaagtg gtatttaagt acaacacaat gtgctaaagt ataataaaat
     1621 aacaagtata atagtaaata gccaccactc attgatggcc aactatgcac aggcaccata
     1681 cttggtgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag
     1741 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     1801 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     1861 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg
     1921 aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg
     1981 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     2041 agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt
     2101 aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc
     2161 ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt
     2221 ccgcttcctt tagcagccct tgcgccctga attttgttaa aattcgcgtt aaatttttgt
     2281 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2341 gagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt ggacaggccg
     2401 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt
     2461 actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt
     2521 ccccagtgca agtgcaggtg ccagaacatt tctctggcct aactggccgg tacctgagct
     2581 cgctagcctc gaggatatca agatctggcc tcggcggcca agcttacccc cgagctgtgc
     2641 tgctcgcggc cgccaccgcc gggccccggc cgtccctggc tcccctcctg cctcgagaag
     2701 ggcagggctt ctcagaggct tggcgggaaa aagaacggag ggagggatcg cgctgagtat
     2761 aaaagccggt tttcggggct ttatctaact cgctgtagta attccggcaa tccggtactg
     2821 ttggtaaagc caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
     2881 cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
     2941 ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt
     3001 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
     3061 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
     3121 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
     3181 acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa
     3241 ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa
     3301 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
     3361 ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca
     3421 tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct
     3481 gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg
     3541 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt
     3601 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga
     3661 gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg
     3721 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg
     3781 gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta
     3841 tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg
     3901 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc
     3961 tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta
     4021 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg
     4081 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg
     4141 accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat
     4201 ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg
     4261 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     4321 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     4381 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     4441 gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtaattc tagagtcggg
     4501 gcggccggcc gcttcgagca gacatgatac gatacattga tgagtttgga caaaccacaa
     4561 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     4621 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     4681 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     4741 aaatcgataa ggatccgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     4801 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     4861 ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4921 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4981 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5041 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5101 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5161 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5221 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5281 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5341 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5401 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5461 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5521 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5581 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5641 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5701 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5761 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5821 tgacagcggc cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca
     5881 ccgatctcag cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag
     5941 atcactacga ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag
     6001 ccgcgttcac cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga
     6061 agaagtggtc ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct
     6121 agagtaagaa gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc
     6181 gtggtatcac gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc
     6241 cgggtcacat gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc
     6301 gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat
     6361 tctcttaccg tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag
     6421 tcgttttgtg agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac
     6481 aacaccgcgc cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg
     6541 cggaaagact caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca
     6601 cccagttgat cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc
     6661 aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc
     6721 gtcctttttc aatattattg aagcatttat cagggttaca ctgcggctcc tcatcgcgat
     6781 aataaacccc
//
