LOCUS       sequence-234010-        2852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2852)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: H1-Mos-6U
FEATURES             Location/Qualifiers
     source          1..2852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..331
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     83..103
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     254..273
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(689..708)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(854..872)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        940..1044
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1045..1113,1114..1905)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1263..1282)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2076..2664
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2565..2584
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2818..2835
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
       61 gtttcccgac tggaaagcaa ttgagggcct atttcccatg attccttcat atttgcatat
      121 acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt
      181 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
      241 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
      301 tttatatatc ttgtggaaag gacgaaacac cgggaaaaag cttgcaccaa ggacctacgt
      361 gcatcccagc ggagtttctg ggctgaactg aacattgcaa gactacgcca cgacaacata
      421 gttcgggttg tggctgccag cacgcgcacg cccgaagact ccaacagcct aggtaccata
      481 atcatggagt ttgggggcaa cgtgactcta caccaagtca tctacggtgc cacccgctca
      541 ccggagcctc tcagctgcag agaacaaccg agtttgggga agtgcctcaa gtattcccta
      601 gatgttgtta gcggccgctt tttccctcta tcactgatag ggaacttata agattcccaa
      661 atccaaagac atttcacgtt tatggtgatt tcccagaaca catagcgaca tgcaaatatt
      721 gcagggcgcc actcccctgt ccctcacagc catcttcctg ccagggcgca cgcgcgctgg
      781 gtgttcccgc ctagtgacac tgggcccgcg attccttgga gcgggttgat gacgtcagcg
      841 ttctgcaggg gcggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      901 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      961 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1021 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1081 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1141 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1201 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1261 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1321 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1381 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1441 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1501 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1561 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     1621 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1681 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     1741 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     1801 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1861 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1921 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1981 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2041 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2101 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2161 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2221 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2281 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2341 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2401 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2461 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2521 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2581 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2641 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2701 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2761 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2821 gagtcagtga gcgaggaagc ggaagagcgc cc
//
