LOCUS       sequence-234017-        8255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Self replication Level T acceptor vector with LacZ compatible 
            blue/white screening. Modified BioBrick vector, RSF1010-derived ori.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie 
            MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick
            AJ
  TITLE     CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria 
            Based on the Plant MoClo Syntax.
  JOURNAL   Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub
            2019 Feb 28.
  PUBMED    30819783
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.18.01401"; journalName: "Plant Physiol"; date: 
            "2019-05"; volume: "180"; issue: "1"; pages: "39-55"
COMMENT     Sequence Label: pCAT.000
FEATURES             Location/Qualifiers
     source          1..8255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    68..89
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(104..109,110..127,128..134)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    142..158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     147..169
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     166..182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     166..182
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             178..501
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    195..251
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(252..269)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(252..268)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(261..283)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(477..496)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     terminator      602..659
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(683..700)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      841..1235
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            1577..1664
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             2903..3874
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             4385..5224
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             5211..6062
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(6185..7000)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6185..7000)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6908..6927
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6992..7011
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(join(7155..7946,7947..8015))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7778..7797
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8016..8120)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8188..8206
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8210..8253)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 tgccttgtct tcgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
       61 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      121 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      181 accatgatta cgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg
      241 agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      301 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      361 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
      421 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
      481 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
      541 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgag aagacaaggg
      601 atccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
      661 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      721 ggcgagcggt atcagctcac tcaaaggcgg taatcaattg agttctttta ccctcagccg
      781 aaatgcctgc cgttgctaga cattgccagc cagtgcccgt cactcccgta ctaactgtca
      841 cgaacccctg caataactgt cacgcccccc tgcaataact gtcacgaacc cctgcaataa
      901 ctgtcacgcc cccaaacctg caaacccagc aggggcgggg gctggcgggg tgttggaaaa
      961 atccatccat gattatctaa gaataatcca ctaggcgcgg ttatcagcgc ccttgtgggg
     1021 cgctgctgcc cttgcccaat atgcccggcc agaggccgga tagctggtct attcgctgcg
     1081 ctaggctaca caccgcccca ccgctgcgcg gcagggggaa aggcgggcaa agcccgctaa
     1141 accccacacc aaaccccgca gaaatacgct ggagcgcttt tagccgcttt agcggccttt
     1201 ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca gggcctgtct cggtcgatca
     1261 ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa caaggcgcgg tcgtggtcgc
     1321 gttcaaggta cgcatccatt gccgccatga gccgatcctc cggccactcg ctgctgttca
     1381 ccttggccaa aatcatggcc cccaccagca ccttgcgcct tgtttcgttc ttgcgctctt
     1441 gctgctgttc ccttgcccgc acccgctgaa tttcggcatt gattcgcgct cgttgttctt
     1501 cgagcttggc cagccgatcc gccgccttgt tgctcccctt aaccatcttg acaccccatt
     1561 gttaatgtgc tgtctcgtag gctatcatgg aggcacagcg gcggcaatcc cgaccctact
     1621 ttgtagggga gggcgcactt accggtttct cttcgagaaa ctggcctaac ggccaccctt
     1681 cgggcggtgc gctctccgag ggccattgca tggagccgaa aagcaaaagc aacagcgagg
     1741 cagcatggcg atttatcacc ttacggcgaa aaccggcagc aggtcgggcg gccaatcggc
     1801 cagggccaag gccgactaca tccagcgcga aggcaagtat gcccgcgaca tggatgaagt
     1861 cttgcacgcc gaatccgggc acatgccgga gttcgtcgag cggcccgccg actactggga
     1921 tgctgccgac ctgtatgaac gcgccaatgg gcggctgttc aaggaggtcg aatttgccct
     1981 gccggtcgag ctgaccctcg accagcagaa ggcgctggcg tccgagttcg cccagcacct
     2041 gaccggtgcc gagcgcctgc cgtatacgct ggccatccat gccggtggcg gcgagaaccc
     2101 gcactgccac ctgatgatct ccgagcggat caatgacggc atcgagcggc ccgccgctca
     2161 gtggttcaag cggtacaacg gcaagacccc ggagaagggc ggggcacaga agaacgaagc
     2221 gctcaagccc aaggcatggc ttgagcagac ccgcgaggca tgggccgacc atgccaaccg
     2281 ggcattagag cgggctggcc acgacgcccg cattgaccac agaacacttg aggcgcaggg
     2341 catcgagcgc ctgcccggtg ttcacctggg gccgaacgtg gtggagatgg aaggccgggg
     2401 catccgcacc gaccgggcag acgtggccct gaacatcgac accgccaacg cccagatcat
     2461 cgacttacag gaataccggg aggcaataga ccatgaacgc aatcgacaga gtgaagaaat
     2521 ccagaggcat caacgagtta gcggagcaga tcgaaccgct ggcccagagc atggcgacac
     2581 tggccgacga agcccggcag gtcatgagcc agacccagca ggccagcgag gcgcaggcgg
     2641 cggagtggct gaaagcccag cgccagacag gggcggcatg ggtggagctg gccaaagagt
     2701 tgcgggaggt agccgccgag gtgagcagcg ccgcgcagag cgcccggagc gcgtcgcggg
     2761 ggtggcactg gaagctatgg ctaaccgtga tgctggcttc catgatgcct acggtggtgc
     2821 tgctgatcgc atcgttgctc ttgctcgacc tgacgccact gacaaccgag gacggctcga
     2881 tctggctgcg cttggtggcc cgatgaagaa cgacaggact ttgcaggcca taggccgaca
     2941 gctcaaggcc atgggctgtg agcgcttcga tatcggcgtc agggacgcca ccaccggcca
     3001 gatgatgaac cgggaatggt cagccgccga agtgctccag aacacgccat ggctcaagcg
     3061 gatgaatgcc cagggcaatg acgtgtatat caggcccgcc gagcaggagc ggcatggtct
     3121 ggtgctggtg gacgacctca gcgagtttga cctggatgac atgaaagccg agggccggga
     3181 gcctgccctg gtagtggaaa ccagcccgaa gaactatcag gcatgggtca aggtggccga
     3241 cgccgcaggc ggtgaacttc gggggcagat tgcccggacg ctggccagcg agtacgacgc
     3301 cgacccggcc agcgccgaca gccgccacta tggccgcttg gcgggcttca ccaaccgcaa
     3361 ggacaagcac accacccgcg ccggttatca gccgtgggtg ctgctgcgtg aatccaaggg
     3421 caagaccgcc accgctggcc cggcgctggt gcagcaggct ggccagcaga tcgagcaggc
     3481 ccagcggcag caggagaagg cccgcaggct ggccagcctc gaactgcccg agcggcagct
     3541 tagccgccac cggcgcacgg cgctggacga gtaccgcagc gagatggccg ggctggtcaa
     3601 gcgcttcggt gatgacctca gcaagtgcga ctttatcgcc gcgcagaagc tggccagccg
     3661 gggccgcagt gccgaggaaa tcggcaaggc catggccgag gccagcccag cgctggcaga
     3721 gcgcaagccc ggccacgaag cggattacat cgagcgcacc gtcagcaagg tcatgggtct
     3781 gcccagcgtc cagcttgcgc gggccgagct ggcacgggca ccggcacccc gccagcgagg
     3841 catggacagg ggcgggccag atttcagcat gtagtgcttg cgttggtact cacgcctgtt
     3901 atactatgag tactcacgca cagaaggggg ttttatggaa tacgaaaaaa gcgcttcagg
     3961 gtcggtctac ctgatcaaaa gtgacaaggg ctattggttg cccggtggct ttggttatac
     4021 gtcaaacaag gccgaggctg gccgcttttc agtcgctgat atggccagcc ttaaccttga
     4081 cggctgcacc ttgtccttgt tccgcgaaca caagcctttc ggccccggca agtttctcgg
     4141 tgactgatat gaaagaccaa aaggacaagc agaccggcga cctgctggcc agccctgacg
     4201 ctgtacgcca agcgcgatat gccgagcgca tgaaggccaa agggatgcgt cagcgcaagt
     4261 tctggctgac cgacgacgaa tacgaggcgc tgcgcgagtg cctggaagaa ctcagagcgg
     4321 cgcagggcgg gggtagtgac cccgccagcg cctaaccacc aactgcctgc aaaggaggca
     4381 atcaatggct acccataagc ctatcaatat tctggaggcg ttcgcagcag cgccgccacc
     4441 gctggactac gttttgccca acatggtggc cggtacggtc ggggcgctgg tgtcgcccgg
     4501 tggtgccggt aaatccatgc tggccctgca actggccgca cagattgcag gcgggccgga
     4561 tctgctggag gtgggcgaac tgcccaccgg cccggtgatc tacctgcccg ccgaagatcc
     4621 gcccaccgcc attcatcacc gcctgcacgc ccttggggcg cacctcagcg ccgaggaacg
     4681 gcaagccgtg gctgacggcc tgctgatcca gccgctgatc ggcagcctgc ccaacatcat
     4741 ggccccggag tggttcgacg gcctcaagcg cgccgccgag ggccgccgcc tgatggtgct
     4801 ggacacgctg cgccggttcc acatcgagga agaaaacgcc agcggcccca tggcccaggt
     4861 catcggtcgc atggaggcca tcgccgccga taccgggtgc tctatcgtgt tcctgcacca
     4921 tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag cagcaggcca gccggggcag
     4981 ctcggtactg gtcgataaca tccgctggca gtcctacctg tcgagcatga ccagcgccga
     5041 ggccgaggaa tggggtgtgg acgacgacca gcgccggttc ttcgtccgct tcggtgtgag
     5101 caaggccaac tatggcgcac cgttcgctga tcggtggttc aggcggcatg acggcggggt
     5161 gctcaagccc gccgtgctgg agaggcagcg caagagcaag ggggtgcccc gtggtgaagc
     5221 ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc ggcgcactgt ctggcccccg
     5281 gcctgttccg tgccctcaag cggggcgagc gcaagcgcag caagctggac gtgacgtatg
     5341 actacggcga cggcaagcgg atcgagttca gcggcccgga gccgctgggc gctgatgatc
     5401 tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa tggcctagtg cttggcccgg
     5461 aacccaagac cgaaggcgga cggcagctcc ggctgttcct ggaacccaag tgggaggccg
     5521 tcaccgctga tgccatggtg gtcaaaggta gctatcgggc gctggcaaag gaaatcgggg
     5581 cagaggtcga tagtggtggg gcgctcaagc acatacagga ctgcatcgag cgcctttgga
     5641 aggtatccat catcgcccag aatggccgca agcggcaggg gtttcggctg ctgtcggagt
     5701 acgccagcga cgaggcggac gggcgcctgt acgtggccct gaaccccttg atcgcgcagg
     5761 ccgtcatggg tggcggccag catgtgcgca tcagcatgga cgaggtgcgg gcgctggaca
     5821 gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg gatcgacccc ggcaaaaccg
     5881 gcaaggcttc catagatacc ttgtgcggct atgtctggcc gtcagaggcc agtggttcga
     5941 ccatgcgcaa gcgccgccag cgggtgcgcg aggcgttgcc ggagctggtc gcgctgggct
     6001 ggacggtaac cgagttcgcg gcgggcaagt acgacatcac ccggcccaag gcggcaggct
     6061 gacccccccc actctattgt aaacaagaca tttttatctt ttatattcaa tggcttattt
     6121 tcctgctaat tggtaatacc atggtaccgc tctgccagtg ttacaaccaa ttaaccaatt
     6181 ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     6241 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     6301 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     6361 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     6421 cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag
     6481 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     6541 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     6601 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     6661 gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg
     6721 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     6781 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     6841 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     6901 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     6961 gcggcctgga gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
     7021 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
     7081 atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga
     7141 aggatcagct cgagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7201 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7261 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7321 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7381 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7441 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7501 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7561 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7621 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7681 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7741 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7801 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7861 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7921 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7981 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8041 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8101 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     8161 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
     8221 aaaaaatcct tagctttcgc taaggatgat ttctg
//
