LOCUS       sequence-23402-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Kaykas A, Yang-Snyder J, Heroux M, Shah KV, Bouvier M, Moon RT
  TITLE     Mutant Frizzled 4 associated with vitreoretinopathy traps wild-type 
            Frizzled in the endoplasmic reticulum by oligomerization.
  JOURNAL   Nat Cell Biol. 2004 Jan . 6(1):52-8.
  PUBMED    14688793
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2004 Jan . 6(1):52-8."
COMMENT     Sequence Label: XE239 Fz1-ER
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           106..145
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     sig_peptide     182..259
                     /codon_start=1
                     /product="human growth hormone signal sequence"
                     /label=hGH signal sequence
                     /translation="MATGSRTSLLLAFGLLCLPWLQEGSA"
     CDS             266..586
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /label=DmrD
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 gggctttagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgggggatct tggtggcgtg
      121 aaactcccgc acctcttcgg ccagcgaatt cagggtcctg tggacagctc acctagctgc
      181 aatggctaca ggctcccgga cgtccctgct cctggctttt ggcctgctct gcctgccctg
      241 gcttcaagag ggcagtgcct ctagaggagt gcaggtggag actatctccc caggagacgg
      301 gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga
      361 tggaaagaaa atggattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa
      421 gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc
      481 caaactgact atatctccag attatgccta tggtgccact gggcacccag gcatcatccc
      541 accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaaacta gaggagtgca
      601 ggtggagact atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt
      661 ggtgcactac accgggatgc ttgaagatgg aaagaaaatg gattcctccc gggacagaaa
      721 caagcccttt aagtttatgc taggcaagca ggaagtgatc cnaagctggg aaaaagggtt
      781 gcccagatga gtgtgggtca aaa
//
