LOCUS       sequence-234020-        6747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDONR for Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, 
            Stewart A, Svejstrup JQ
  TITLE     SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.
  JOURNAL   Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038.
  PUBMED    31104839
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038."
COMMENT     Sequence Label: pDONR223 SCAF8_isoC_CRISPR resistant
FEATURES             Location/Qualifiers
     source          1..6747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(270..297)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(389..475)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     524..546
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     538..555
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     539..555
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    571..670
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4628..4727)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4744..4763)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4745..4763)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4768..4784)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4768..4784)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5215..6006
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6099..6687
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6588..6607
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
      241 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
      301 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
      361 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
      421 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
      481 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
      541 aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg actgatagtg
      601 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      661 aaagcaggct tcaccatgcc gcagccgctg ctgccagcgc ttcctccttt aagcagcccg
      721 tcaggggctg gaagctgtgg accatcagga ggggagtgca gtggccgccg cctcttccgc
      781 cgccgggctc ggggcctccg cagcgacaac atggaggccg tgaagacctt caatagcgag
      841 ttgtattccc tgaatgacta taaaccaccc atttcgaaag cgaaaatgac ccaaattact
      901 aaggcagcca tcaaagctat taagttctat aaacatgtgg tacagagtgt tgagaagttt
      961 attcagaaat gtaaaccaga atacaaagta cctggacttt atgttattga ctccattgtg
     1021 cgacaatccc gacatcagtt tggtcaagaa aaggatgtgt ttgcacccag atttagtaat
     1081 aacatcatta gcactttcca gaatttatat cgttgccctg gggatgacaa gagtaaaata
     1141 gtgagagtac taaacttatg gcagaagaat aatgtattta agagtgagat tattcagccc
     1201 cttttggata tggcagccgg gattccgcct ccagttgtca cacctgtttt ggccagcact
     1261 accactgcta tgagcaatac tccaggaact ccagtcacgc cggtcacgcc tgccaatgtg
     1321 gttcagggat tgccagaccc atgggtatct cagataacaa atacagatac acttgcggct
     1381 gtagctcaga tcttgcaaag tcctcaaggc cagcagcttc aacaattaat acaaacctta
     1441 cagatacaac aacagaagcc ccagccttcc attctgcagg ccctagatgc tggtcttgtt
     1501 gttcagttgc aagctcttac ggcacaactt acagctgcag ctgcagctgc caacactctt
     1561 actcccttag aacagggagt ctcctttaac aagaagttga tggataggtt tgattttggg
     1621 gaagactctg agcatagtga agaacccaaa aaggaaattc cagcttcaca actttctcac
     1681 gtttcagaat ctgtgaacaa ttccattttt catcagatag cagaacaact acaacagcaa
     1741 aacctagaac atctcagaca gcagctcttg gagcagcaac agcctcaaaa ggccactcct
     1801 caggatagtc aggaaggaac ctttgggtca gagcattcag cgtcaccatc acaagggagt
     1861 agtcagcagc attttcttga acctgaagtc aatttggatg attccataga tattcagcaa
     1921 caggatatgg atatagatga agggcaagat ggagtggaag aggaggtctt tgaacaagaa
     1981 gctaagaaag tggcggttcg ctcaagatca agaacacatt cacgatctcg ttcaagatca
     2041 ccaagaaaac gaaggtctag gtcacggtct ggctctagaa agcgtaaaca cagaaagcga
     2101 tcacgctccc gctcaagaga aagaaagagg aaatcatcac ggtcgtattc aagtgaaagg
     2161 agagccagag aaagggagaa agaacgacag aaaaagggat tacctccaat tagatctaaa
     2221 acactaagtg tatgtagtac tactctctgg gttgggcaag tggacaagaa ggcaacacag
     2281 caagacttaa ccaacctgtt tgaagagttt ggacagattg aatccattaa tatgattcct
     2341 ccgcgaggct gtgcttatgt ctgcatggtt catcgacaag atgcatttcg agctcttcag
     2401 aaactcagtt ctggatcata taaaattggg tccaaggtca ttaagatcgc ttgggcttta
     2461 aacaaaggtg taaaaacaga atacaaacaa ttctgggatg tggatcttgg agttacatat
     2521 ataccatggg aaaaagttaa agtggatgac ttggaaggtt ttgcagaagg aggcatgatt
     2581 gatcaggaga ctgtaaatac tgagtgggaa actgtgaaaa gctcagaacc tgttaaagag
     2641 acggtccaga caactcagag cccaactcca gttgaaaagg agacagtggt cacaacccag
     2701 gcagaggttt tccctcctcc tgttgctatg ttgcagattc cagtggcgcc agccgtgcct
     2761 acagttagtt tagtcccacc agcatttcct gtgtcgatgc cggttcctcc tcctggattc
     2821 agtccaatcc ctccacctcc ttttttaaga gcaagtttta acccttcaca accaccacct
     2881 ggtttcatgc cgcctccagt tcccccacct gttgtgccac cccctacgat tccaccagta
     2941 gtaccaacat ctttagtgca gccgtcatta tccatgacac cggaaactgt gaaagatgtt
     3001 ggatttggta gccttgttat accaggcggt tctgttgcca gcaatcttgc tacttccgct
     3061 ctgccagctg gaaatgtttt taatgctcca actaaacagg cagagcctga agaaaaagta
     3121 cctcatctta tagaccacca gatttcttct ggtgaaaaca ccagatcagt gattccaaat
     3181 gatatttcaa gtaatgctgc aattttagga ggacagccgc caaatgtgac aagcaattct
     3241 ggaattctgg gagtccaaag accaaatgta tcaagtaatt ctgaaattct tggggtccgg
     3301 ccatctaatg tttccagtag ttctgggatt attgcagccc aaccaccaaa tattctaaat
     3361 aactctggaa tattgggaat acagccaccc agtgtgtcaa atagttctgg acttttggga
     3421 gtgctacccc caaatatacc taacaattct ggacttgtag gagtacagcc accaaatgtt
     3481 ccaaatactc ctggacttct gggaacacag ccaccagctg gacctcaaaa cttaccccct
     3541 ttaagtatcc ctaatcaaag gatgcccaca atgccaatgt tagacattcg tccgggacta
     3601 ataccacagg cacctgggcc aagattccct ttaatacagc ctggaattcc accccaacgg
     3661 ggaatcccac ccccatcggt acttgattca gctcttcatc caccaccccg tggacctttt
     3721 cctccaggag atatttttag tcaaccagaa agaccttttt tagctcctgg aagacaaagc
     3781 gtagacaatg ttactaaccc agaaaaaagg ataccacttg ggaatgataa cattcaacag
     3841 gaaggagata gagattaccg gtttcctcct atagaaacca gggaaagcat tagtagacct
     3901 ccccctgtgg atgttagaga tgtggttggg cggcctatag atccaagaga aggtcctgga
     3961 cggcctccac tagatggtag ggatcatttt ggaagacctc ctgtagatat aagagagaat
     4021 cttgtgaggc caggtataga tcatcttggt cgaagagacc actttggctt taatccagag
     4081 aagccctggg ggcatagagg agattttgat gagagagagc atcgggttct accggtctat
     4141 ggtggtccaa aaggcttaca tgaagaaaga ggtagatttc ggtctggaaa ctatcgattt
     4201 gatcctagaa gtggtccttg gaaccgagga tttggacaag aagttcacag agattttgat
     4261 gaccgcagaa gaccctggga gaggcaaagg gatagggatg acagagattt tgatttctgc
     4321 agagaaatga atggaaatcg tcttggacga gacagaattc aaaacacttg ggttccccct
     4381 cctcatgctc gggtttttga ttattttgaa ggggccactt ctcaacgaaa aggtgataat
     4441 gtgcctcagg ttaatggtga aaatacagag agacatgctc agccaccacc tataccagta
     4501 cagaatgatc ctgaacttta tgaaaaactg acatcttcaa atgaaataaa caaggagaag
     4561 agtgacacag ttgctgatat agaaagtgaa ccagtggtag aaagcacaga aactgagggg
     4621 acataaaacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     4681 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     4741 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     4801 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag
     4861 accagccagg acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca
     4921 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     4981 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     5041 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     5101 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     5161 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg
     5221 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     5281 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     5341 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     5401 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     5461 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     5521 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     5581 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     5641 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     5701 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     5761 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     5821 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     5881 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     5941 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     6001 aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     6061 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6121 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6181 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6241 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6301 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6361 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6421 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6481 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6541 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6601 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6661 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6721 gccttttgct ggccttttgc tcacatg
//
