LOCUS       sequence-234022-        6612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDONR for Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, 
            Stewart A, Svejstrup JQ
  TITLE     SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.
  JOURNAL   Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038.
  PUBMED    31104839
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038."
COMMENT     Sequence Label: pDONR223 SCAF8_isoD_CRISPR_resistant
FEATURES             Location/Qualifiers
     source          1..6612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4491..4590)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4607..4626)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4608..4626)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4631..4647)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4631..4647)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5078..5869
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5962..6550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6451..6470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc accatggagg ccgtgaagac cttcaatagc gagttgtatt ccctgaatga
      721 ctataaacca cccatttcga aagcgaaaat gacccaaatt actaaggcag ccatcaaagc
      781 tattaagttc tataaacatg tggtacagag tgttgagaag tttattcaga aatgtaaacc
      841 agaatacaaa gtacctggac tttatgttat tgactccatt gtgcgacaat cccgacatca
      901 gtttggtcaa gaaaaggatg tgtttgcacc cagatttagt aataacatca ttagcacttt
      961 ccagaattta tatcgttgcc ctggggatga caagagtaaa atagtgagag tactaaactt
     1021 atggcagaag aataatgtat ttaagagtga gattattcag ccccttttgg atatggcagc
     1081 cgggattccg cctccagttg tcacacctgt tttggccagc actaccactg ctatgagcaa
     1141 tactccagga actccagtca cgccggtcac gcctgccaat gtggttcagg gattgccaga
     1201 cccatgggta tctcagataa caaatacaga tacacttgcg gctgtagctc agatcttgca
     1261 aagtcctcaa ggccagcagc ttcaacaatt aatacaaacc ttacagatac aacaacagaa
     1321 gccccagcct tccattctgc aggccctaga tgctggtctt gttgttcagt tgcaagctct
     1381 tacggcacaa cttacagctg cagctgcagc tgccaacact cttactccct tagaacaggg
     1441 agtctccttt aacaagaagt tgatggatag gtttgatttt ggggaagact ctgagcatag
     1501 tgaagaaccc aaaaaggaaa ttccagcttc acaactttct cacgtttcag aatctgtgaa
     1561 caattccatt tttcatcaga tagcagaaca actacaacag caaaacctag aacatctcag
     1621 acagcagctc ttggagcagc aacagcctca aaaggccact cctcaggata gtcaggaagg
     1681 aacctttggg tcagagcatt cagcgtcacc atcacaaggg agtagtcagc agcattttct
     1741 tgaacctgaa gtcaatttgg atgattccat agatattcag caacaggata tggatataga
     1801 tgaagggcaa gatggagtgg aagaggaggt ctttgaacaa gaagctaaga aagtggcggt
     1861 tcgctcaaga tcaagaacac attcacgatc tcgttcaaga tcaccaagaa aacgaaggtc
     1921 taggtcacgg tctggctcta gaaagcgtaa acacagaaag cgatcacgct cccgctcaag
     1981 agaaagaaag aggaaatcat cacggtcgta ttcaagtgaa aggagagcca gagaaaggga
     2041 gaaagaacga cagaaaaagg gattacctcc aattagatct aaaacactaa gtgtatgtag
     2101 tactactctc tgggttgggc aagtggacaa gaaggcaaca cagcaagact taaccaacct
     2161 gtttgaagag tttggacaga ttgaatccat taatatgatt cctccgcgag gctgtgctta
     2221 tgtctgcatg gttcatcgac aagatgcatt tcgagctctt cagaaactca gttctggatc
     2281 atataaaatt gggtccaagg tcattaagat cgcttgggct ttaaacaaag gtgtaaaaac
     2341 agaatacaaa caattctggg atgtggatct tggagttaca tatataccat gggaaaaagt
     2401 taaagtggat gacttggaag gttttgcaga aggaggcatg attgatcagg agactgtaaa
     2461 tactgagtgg gaaactgtga aaagctcaga acctgttaaa gagacggtcc agacaactca
     2521 gagcccaact ccagttgaaa aggagacagt ggtcacaacc caggcagagg ttttccctcc
     2581 tcctgttgct atgttgcaga ttccagtggc gccagccgtg cctacagtta gtttagtccc
     2641 accagcattt cctgtgtcga tgccggttcc tcctcctgga ttcagtccaa tccctccacc
     2701 tcctttttta agagcaagtt ttaacccttc acaaccacca cctggtttca tgccgcctcc
     2761 agttccccca cctgttgtgc caccccctac gattccacca gtagtaccaa catctttagt
     2821 gcagccgtca ttatccatga caccggaaac tgtgaaagat gttggatttg gtagccttgt
     2881 tataccaggc ggttctgttg ccagcaatct tgctacttcc gctctgccag ctggaaatgt
     2941 ttttaatgct ccaactaaac aggcagagcc tgaagaaaaa gtacctcatc ttatagacca
     3001 ccagatttct tctggtgaaa acaccagatc agtgattcca aatgatattt caagtaatgc
     3061 tgcaatttta ggaggacagc cgccaaatgt gacaagcaat tctggaattc tgggagtcca
     3121 aagaccaaat gtatcaagta attctgaaat tcttggggtc cggccatcta atgtttccag
     3181 tagttctggg attattgcag cccaaccacc aaatattcta aataactctg gaatattggg
     3241 aatacagcca cccagtgtgt caaatagttc tggacttttg ggagtgctac ccccaaatat
     3301 acctaacaat tctggacttg taggagtaca gccaccaaat gttccaaata ctcctggact
     3361 tctgggaaca cagccaccag ctggacctca aaacttaccc cctttaagta tccctaatca
     3421 aaggatgccc acaatgccaa tgttagacat tcgtccggga ctaataccac aggcacctgg
     3481 gccaagattc cctttaatac agcctggaat tccaccccaa cggggaatcc cacccccatc
     3541 ggtacttgat tcagctcttc atccaccacc ccgtggacct tttcctccag gagatatttt
     3601 tagtcaacca gaaagacctt ttttagctcc tggaagacaa agcgtagaca atgttactaa
     3661 cccagaaaaa aggataccac ttgggaatga taacattcaa caggaaggag atagagatta
     3721 ccggtttcct cctatagaaa ccagggaaag cattagtaga cctccccctg tggatgttag
     3781 agatgtggtt gggcggccta tagatccaag agaaggtcct ggacggcctc cactagatgg
     3841 tagggatcat tttggaagac ctcctgtaga tataagagag aatcttgtga ggccaggtat
     3901 agatcatctt ggtcgaagag accactttgg ctttaatcca gagaagccct gggggcatag
     3961 aggagatttt gatgagagag agcatcgggt tctaccggtc tatggtggtc caaaaggctt
     4021 acatgaagaa agaggtagat ttcggtctgg aaactatcga tttgatccta gaagtggtcc
     4081 ttggaaccga ggatttggac aagaagttca cagagatttt gatgaccgca gaagaccctg
     4141 ggagaggcaa agggataggg atgacagaga ttttgatttc tgcagagaaa tgaatggaaa
     4201 tcgtcttgga cgagacagaa ttcaaaacac ttgggttccc cctcctcatg ctcgggtttt
     4261 tgattatttt gaaggggcca cttctcaacg aaaaggtgat aatgtgcctc aggttaatgg
     4321 tgaaaataca gagagacatg ctcagccacc acctatacca gtacagaatg atcctgaact
     4381 ttatgaaaaa ctgacatctt caaatgaaat aaacaaggag aagagtgaca cagttgctga
     4441 tatagaaagt gaaccagtgg tagaaagcac agaaactgag gggacataaa acccagcttt
     4501 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     4561 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     4621 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     4681 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa
     4741 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     4801 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     4861 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     4921 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     4981 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     5041 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
     5101 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     5161 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     5221 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     5281 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     5341 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     5401 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     5461 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     5521 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     5581 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     5641 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     5701 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     5761 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     5821 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     5881 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     5941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
     6361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6601 tgctcacatg tt
//
