LOCUS       sequence-234025-        9848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible GFP (N-terminal tag) SCAF8 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, 
            Stewart A, Svejstrup JQ
  TITLE     SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.
  JOURNAL   Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038.
  PUBMED    31104839
REFERENCE   2  (bases 1 to 9848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038."
COMMENT     Sequence Label: pFRT/TO/GFP-SCAF8 (CRISPR_resistant)
FEATURES             Location/Qualifiers
     source          1..9848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..42
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(86..105)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        277..656
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        657..860
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     811..831
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    862..880
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    883..901
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     911..935
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1032..1034,1035..1037,1038..1748)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1032..1034,1035..1037,1038..1748)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1077..1098)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1338..1357)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1685..1706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1755..1779
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5602..5626)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5679..5720
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5800..5817)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5806..6030
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6163..6182)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6314..6361
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6369..7391
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7521..7642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7558..7577)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7612..7631
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7691..7707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7691..7707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7704..7726)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7715..7731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7739..7745,7746..7763,7764..7769))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7784..7805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7922..7939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8093..8681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8173..8192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8852..9643,9644..9712))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9475..9494
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9713..9817)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cggacggatc gggagatctc
       61 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
      121 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
      181 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
      241 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      301 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      361 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      421 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      481 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      541 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      601 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      661 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      721 gtctccaccc cattgacgtc aatgggagtt tgttttggaa ccaaaatcaa cgggactttc
      781 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      841 aggtctatat aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag
      901 atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
      961 ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta
     1021 agcttggtac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1081 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1141 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1201 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     1261 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1321 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1381 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1441 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     1501 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     1561 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1621 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1681 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1741 tgtacaagaa gcttacaagt ttgtacaaaa aagcaggctt caccatggag gccgtgaaga
     1801 ccttcaatag cgagttgtat tccctgaatg actataaacc acccatttcg aaagcgaaaa
     1861 tgacccaaat tactaaggca gccatcaaag ctattaagtt ctataaacat gtggtacaga
     1921 gtgttgagaa gtttattcag aaatgtaaac cagaatacaa agtacctgga ctttatgtta
     1981 ttgactccat tgtgcgacaa tcccgacatc agtttggtca agaaaaggat gtgtttgcac
     2041 ccagatttag taataacatc attagcactt tccagaattt atatcgttgc cctggggatg
     2101 acaagagtaa aatagtgaga gtactaaact tatggcagaa gaataatgta tttaagagtg
     2161 agattattca gccccttttg gatatggcag ccgggattcc gcctccagtt gtcacacctg
     2221 ttttggccag cactaccact gctatgagca atactccagg aactccagtc acgccggtca
     2281 cgcctgccaa tgtggttcag ggattgccag acccatgggt atctcagata acaaatacag
     2341 atacacttgc ggctgtagct cagatcttgc aaagtcctca aggccagcag cttcaacaat
     2401 taatacaaac cttacagata caacaacaga agccccagcc ttccattctg caggccctag
     2461 atgctggtct tgttgttcag ttgcaagctc ttacggcaca acttacagct gcagctgcag
     2521 ctgccaacac tcttactccc ttagaacagg gagtctcctt taacaagaag ttgatggata
     2581 ggtttgattt tggggaagac tctgagcata gtgaagaacc caaaaaggaa attccagctt
     2641 cacaactttc tcacgtttca gaatctgtga acaattccat ttttcatcag atagcagaac
     2701 aactacaaca gcaaaaccta gaacatctca gacagcagct cttggagcag caacagcctc
     2761 aaaaggccac tcctcaggat agtcaggaag gaacctttgg gtcagagcat tcagcgtcac
     2821 catcacaagg gagtagtcag cagcattttc ttgaacctga agtcaatttg gatgattcca
     2881 tagatattca gcaacaggat atggatatag atgaagggca agatggagtg gaagaggagg
     2941 tctttgaaca agaagctaag aaagtggcgg ttcgctcaag atcaagaaca cattcacgat
     3001 ctcgttcaag atcaccaaga aaacgaaggt ctaggtcacg gtctggctct agaaagcgta
     3061 aacacagaaa gcgatcacgc tcccgctcaa gagaaagaaa gaggaaatca tcacggtcgt
     3121 attcaagtga aaggagagcc agagaaaggg agaaagaacg acagaaaaag ggattacctc
     3181 caattagatc taaaacacta agtgtatgta gtactactct ctgggttggg caagtggaca
     3241 agaaggcaac acagcaagac ttaaccaacc tgtttgaaga gtttggacag attgaatcca
     3301 ttaatatgat tcctccgcga ggctgtgctt atgtctgcat ggttcatcga caagatgcat
     3361 ttcgagctct tcagaaactc agttctggat catataaaat tgggtccaag gtcattaaga
     3421 tcgcttgggc tttaaacaaa ggtgtaaaaa cagaatacaa acaattctgg gatgtggatc
     3481 ttggagttac atatatacca tgggaaaaag ttaaagtgga tgacttggaa ggttttgcag
     3541 aaggaggcat gattgatcag gagactgtaa atactgagtg ggaaactgtg aaaagctcag
     3601 aacctgttaa agagacggtc cagacaactc agagcccaac tccagttgaa aaggagacag
     3661 tggtcacaac ccaggcagag gttttccctc ctcctgttgc tatgttgcag attccagtgg
     3721 cgccagccgt gcctacagtt agtttagtcc caccagcatt tcctgtgtcg atgccggttc
     3781 ctcctcctgg attcagtcca atccctccac ctcctttttt aagagcaagt tttaaccctt
     3841 cacaaccacc acctggtttc atgccgcctc cagttccccc acctgttgtg ccacccccta
     3901 cgattccacc agtagtacca acatctttag tgcagccgtc attatccatg acaccggaaa
     3961 ctgtgaaaga tgttggattt ggtagccttg ttataccagg cggttctgtt gccagcaatc
     4021 ttgctacttc cgctctgcca gctggaaatg tttttaatgc tccaactaaa caggcagagc
     4081 ctgaagaaaa agtacctcat cttatagacc accagatttc ttctggtgaa aacaccagat
     4141 cagtgattcc aaatgatatt tcaagtaatg ctgcaatttt aggaggacag ccgccaaatg
     4201 tgacaagcaa ttctggaatt ctgggagtcc aaagaccaaa tgtatcaagt aattctgaaa
     4261 ttcttggggt ccggccatct aatgtttcca gtagttctgg gattattgca gcccaaccac
     4321 caaatattct aaataactct ggaatattgg gaatacagcc acccagtgtg tcaaatagtt
     4381 ctggactttt gggagtgcta cccccaaata tacctaacaa ttctggactt gtaggagtac
     4441 agccaccaaa tgttccaaat actcctggac ttctgggaac acagccacca gctggacctc
     4501 aaaacttacc ccctttaagt atccctaatc aaaggatgcc cacaatgcca atgttagaca
     4561 ttcgtccggg actaatacca caggcacctg ggccaagatt ccctttaata cagcctggaa
     4621 ttccacccca acggggaatc ccacccccat cggtacttga ttcagctctt catccaccac
     4681 cccgtggacc ttttcctcca ggagatattt ttagtcaacc agaaagacct tttttagctc
     4741 ctggaagaca aagcgtagac aatgttacta acccagaaaa aaggatacca cttgggaatg
     4801 ataacattca acaggaagga gatagagatt accggtttcc tcctatagaa accagggaaa
     4861 gcattagtag acctccccct gtggatgtta gagatgtggt tgggcggcct atagatccaa
     4921 gagaaggtcc tggacggcct ccactagatg gtagggatca ttttggaaga cctcctgtag
     4981 atataagaga gaatcttgtg aggccaggta tagatcatct tggtcgaaga gaccactttg
     5041 gctttaatcc agagaagccc tgggggcata gaggagattt tgatgagaga gagcatcggg
     5101 ttctaccggt ctatggtggt ccaaaaggct tacatgaaga aagaggtaga tttcggtctg
     5161 gaaactatcg atttgatcct agaagtggtc cttggaaccg aggatttgga caagaagttc
     5221 acagagattt tgatgaccgc agaagaccct gggagaggca aagggatagg gatgacagag
     5281 attttgattt ctgcagagaa atgaatggaa atcgtcttgg acgagacaga attcaaaaca
     5341 cttgggttcc ccctcctcat gctcgggttt ttgattattt tgaaggggcc acttctcaac
     5401 gaaaaggtga taatgtgcct caggttaatg gtgaaaatac agagagacat gctcagccac
     5461 cacctatacc agtacagaat gatcctgaac tttatgaaaa actgacatct tcaaatgaaa
     5521 taaacaagga gaagagtgac acagttgctg atatagaaag tgaaccagtg gtagaaagca
     5581 cagaaactga ggggacataa aacccagctt tcttgtacaa agtggttgat atccagcaca
     5641 gtggcggccg ctcaagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     5701 tcctcggtct cgattctacg cgtaccggat ccgcggccgc atagataact gatccagtgt
     5761 gcctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     5821 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     5881 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     5941 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6001 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6061 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6121 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6181 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6241 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6301 gttcacgtac ctagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
     6361 ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     6421 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     6481 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     6541 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     6601 acattgggga gttcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     6661 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     6721 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     6781 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     6841 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     6901 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     6961 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     7021 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     7081 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     7141 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     7201 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     7261 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     7321 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     7381 caaaggaata gcacgtacta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     7441 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     7501 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     7561 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7621 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     7681 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7741 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7801 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7861 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7921 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7981 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8041 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8101 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8161 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8221 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8281 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8341 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8401 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8461 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8521 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8581 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8641 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8701 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8761 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8821 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8881 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8941 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9001 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9061 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9121 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9181 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9241 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9301 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9361 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9421 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9481 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9541 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9601 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9661 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9721 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9781 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9841 ctgacgtc
//
