LOCUS       sequence-234029-        7981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Rivers A, Gietzen KF, Vielhaber E, Virshup DM
  TITLE     Regulation of casein kinase I epsilon and casein kinase I delta by 
            an in vivo futile phosphorylation cycle.
  JOURNAL   J Biol Chem. 1998 Jun 26. 273(26):15980-4.
  PUBMED    9632646
REFERENCE   2  (bases 1 to 7981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Jun 26. 273(26):15980-4."
COMMENT     Sequence Label: V406 4HA-CKIe(K38R)
FEATURES             Location/Qualifiers
     source          1..7981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..146
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             189..1226
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1224..1243)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1268..1316
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1970..2349
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2350..2553
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2504..2524
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2550..2574
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2607..2633
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2649..2738
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     polyA_signal    4035..4169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4059..4078)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4113..4132
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4312..4334)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4894..6683
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg
       61 gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc
      121 gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cccggggggc
      181 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
      241 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
      301 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
      361 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
      421 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
      481 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
      541 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
      601 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
      661 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
      721 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
      781 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
      841 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
      901 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
      961 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     1021 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     1081 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     1141 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg
     1201 atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     1261 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
     1321 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     1381 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     1441 agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta
     1501 gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt
     1561 gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt ggatggcctg
     1621 ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc
     1681 cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg accaattctc
     1741 atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag
     1801 aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc
     1861 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat
     1921 cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg acattgatta
     1981 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     2041 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2101 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2161 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2221 atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2281 cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2341 attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca
     2401 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     2461 caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg
     2521 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatctctaga
     2581 agctgggtac cacgcgtcat atggcttacc catacgatgt tccagattac gctagcggcc
     2641 gcatctttta cccatacgat gttcctgact atgcgggcta tccctatgac gtcccggact
     2701 atgcaggatc ctatccatat gacgttccag attacgctgc tcagtgcggc cgcgagctac
     2761 gtgtggggaa caagtaccgc ctgggacgga agatcgggag cgggtccttc ggagatatct
     2821 acctgggtgc caacatcgcc tctggtgagg aagtcgccat ccggctggag tgtgtgaaga
     2881 caaagcaccc ccagctgcac atcgagagca agttctacaa gatgatgcag ggtggcgtgg
     2941 ggatcccgtc catcaagtgg tgcggagctg agggcgacta caacgtgatg gtcatggagc
     3001 tgctggggcc tagcctcgag gacctgttca acttctgttc ccgcaaattc agcctcaaga
     3061 cggtgctgct cttggccgac cagatgatca gccgcatcga gtatatccac tccaagaact
     3121 tcatccaccg ggacgtcaag cccgacaact tcctcatggg gctggggaag aagggcaacc
     3181 tggtctacat catcgacttc ggcctggcca agaagtaccg ggacgcccgc acccaccagc
     3241 acattcccta ccgggaaaac aagaacctga ccggcacggc ccgctacgct tccatcaaca
     3301 cgcacctggg cattgagcaa agccgtcgag atgacctgga gagcctgggc tacgtgctca
     3361 tgtacttcaa cctgggctcc ctgccctggc aggggctcaa agcagccacc aagcgccaga
     3421 agtatgaacg gatcagcgag aagaagatgt caacgcccat cgaggtcctc tgcaaaggct
     3481 atccctccga attctcaaca tacctcaact tctgccgctc cctgcggttt gacgacaagc
     3541 ccgactactc ttacctacgt cagctcttcc gcaacctctt ccaccggcag ggcttctcct
     3601 atgactacgt ctttgactgg aacatgctga aattcggtgc agcccggaat cccgaggatg
     3661 tggaccggga gcggcgagaa cacgaacgcg aggagaggat ggggcagcta cgggggtccg
     3721 cgacccgagc cctgccccct ggcccaccca cgggggccac tgccaaccgg ctccgcagtg
     3781 ccgccgagcc cgtggcttcc acgccagcct cccgcatcca gccggctggc aatacttctc
     3841 ccagagcgat ctcgcgggtc gaccgggaga ggaaggtgag tatgaggctg cacaggggtg
     3901 cgcccgccaa cgtctcctcc tcagacctca ctgggcggca agaggtctcc cggatcccag
     3961 cctcacagac aagtgtgcca tttgaccatc tcgggaagtg aggagagccc ccattggacc
     4021 ggccggcaag gccggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     4081 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     4141 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     4201 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     4261 tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     4321 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     4381 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga ggtcgactct
     4441 agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct gccccttctt
     4501 cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac tgggccctgt
     4561 tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt agttgacatc
     4621 cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg agcagacttt
     4681 gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct gaagctctta
     4741 caccaatgct gggggacatg tacctcccag gggcccagga agactacggg aggctacacc
     4801 aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc
     4861 aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag
     4921 tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat
     4981 atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat
     5041 gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta
     5101 gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc
     5161 tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg
     5221 tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg
     5281 gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc
     5341 aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg
     5401 gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac
     5461 acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa
     5521 gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga
     5581 cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc
     5641 aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg
     5701 cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa
     5761 cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta
     5821 atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg
     5881 ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg
     5941 gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata
     6001 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcataggct
     6061 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     6121 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     6181 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     6241 atcctaatag agattagggt agtatatgct atcctaattt atatctgggt agcatatact
     6301 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc
     6361 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     6421 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     6481 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     6541 taatctgtat ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc
     6601 agtagtagag tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat
     6661 tttcgctgct tgtccttttc ctgctggttg ctcccattct taggtgaatt taaggaggcc
     6721 aggctaaagc cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat gttttccaac
     6781 gcgagaaggt gttgagcgcg gagctgagtg acgtgacaac atgggtatgc ccaattgccc
     6841 catgttggga ggacgaaaat ggtgacaaga cagatggcca gaaatacacc aacagcacgc
     6901 atgatgtcta ctggggattt attctttagt gcgggggaat acacggcttt taatacgatt
     6961 gagggcgtct cctaacaagt tacatcactc ctgcccttcc tcaccctcat ctccatcacc
     7021 tccttcatct ccgtcatctc cgtcatcacc ctccgcggca gccccttcca ccataggtgg
     7081 aaaccaggga ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc tgatattgca
     7141 ggtaggagcg ggctttgtca taacaaggtc cttaatcgca tccttcaaaa cctcagcaaa
     7201 tatatgagtt tgtaaaaaga ccatgaaata acagacaatg gactccctta gcgggccagg
     7261 ttgtgggccg ggtccagggg ccattccaaa ggggagacga ctcaatggtg taagacgaca
     7321 ttgtggaata gcaagggcag ttcctcgcct taggttgtaa agggaggtct tactacctcc
     7381 atatacgaac acaccggcga cccaagttcc ttcgtcggta gtcctttcta cgtgactcct
     7441 agccaggaga gctcttaaac cttctgcaat gttctcaaat ttcgggttgg aacctccttg
     7501 accacgatgc tttccaaacc accctccttt tttgcgcctg cctccatcac cctgaccccg
     7561 gggtccagtg cttgggcctt ctcctgggtc atctgcgggg ccctgctcta tcgctcccgg
     7621 gggcacgtca ggctcaccat ctgggccacc ttcttggtgg tattcaaaat aatcggcttc
     7681 ccctacaggg tggaaaaatg gccttctacc tggagggggc ctgcgcggtg gagacccgga
     7741 tgatgatgac tgactactgg gactcctggg cctcttttct ccacgtccac gacctctccc
     7801 cctggctctt tcacgacttc cccccctggc tctttcacgt cctctacccc ggcggcctcc
     7861 actacctcct cgaccccggc ctccactacc tcctcgaccc cggcctccac tgcctcctcg
     7921 accccggcct ccacctcctg ctcctscysc tcctgcycct cctcctgctc ctgcccctcc
     7981 t
//
