LOCUS       sequence-234037-        9588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9588)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 9588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_018760-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..9588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4746..4763
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4815..4870
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4869..4888)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7106..7124)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7192..7296
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7297..7365,7366..8157)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7515..7534)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8328..8916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8817..8836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9070..9087
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9204..9225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9240..9245,9246..9263,9264..9270)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9278..9294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9283..9305
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9302..9318
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9302..9318
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccaatttca
     1201 agacctgcga ccagagcagc ttctgcaccc ggcagagaca gatcaagcct ggcgaactgg
     1261 cccactggct ggaacccagc agcgtgaaga tcaaccccta cggcatgacc gccgtgatct
     1321 acaccggcga cctgaaccac agcctgagcc tggacatcac ctacaaccag cacagcatct
     1381 tccacgtgct gatcaacgag ctgaccgacg agtaccggcg gttcagagtg cctacaggcg
     1441 acgtgctgct gagcgtgcag aaagctcctg tgcggagcgt gttcgccgag gactctctgg
     1501 tgctggatgg ccagtctgga cacaaggccg tggtgtacta caaccccttc cacatcgact
     1561 ttctgctgaa cgacgtggtg gtggccagcg ccaacaagcg gagcctgctg aactttgagc
     1621 accagcggaa gaagcccatc atcagcaaga acagcgtgtc ccagaacgac aaccacaccc
     1681 tgcccagcga gaaccacgag gtggacatca gcgccgtgaa gtccagcgag gacagcagcg
     1741 gaaataccgg cgtgtccaag gacagcacca ccgacgagga caaagagaac gagaagcagg
     1801 accacgtggg caccgagcac tctgcccctg aggaacagtc ttggagcgag tacttcaaag
     1861 agcacctgga caccagaccc cacggcctga acagcatcag catggacttc gattttcaag
     1921 gcttcagcca cgtgtacggc atccccgagc acgccgatgg cttcgtgctg aagtctacca
     1981 gcgacggcga cccctaccgg ctgtacaacc tggacgtgtt cgagtacgag gtgtacaaca
     2041 agatggccct gtacggcagc gtgcccgtga tgtggggcca caaccagaac aacaccgtgg
     2101 gcgtgctgtg gctgaacccc agcgaaactt gggtggacat tgactacagc aactccaaga
     2161 actccggcat cttcagcggc ttttttagca agaagcccga gaagtcctcc gtgcagaccc
     2221 ggtggatgag cgagtctggc ctggtggatc tgtacgtgtt catgagcagc acccccagcg
     2281 acgccatgag aagctacgcc tctgtcgtgg gaaccaccaa gctgccccct ctgttcgcca
     2341 tcggctacca ccagtgccgg tggaactaca acgacgaggc cgacctgctg tccgtggata
     2401 agcacttcga tgagtacgag atgcctgtgg atgtgctgtg gctggacatc gagcacaccg
     2461 acggcaagcg gtacttcacc tgggaccgga ccaagttccc caaccccaaa gaaatggtgg
     2521 acaagctgaa cgtgaagggc cggaagctcg tgaccgtggt ggaccctcac atcaagcggg
     2581 accccaactg gcccctgttc agcaacagcc agaacaatgg catcttcgtg aaaacccggg
     2641 acggcaccga gttcgacggc tggtgttggc ctggaagcag cgcctggccc gacttcaccg
     2701 ataagtccgt gcagcagtgg tggtccaacc tgttcctgac ctacgagccc gtgtgcaagg
     2761 actccatgtt cacctggaac gacatgggcg agcccagcgt gttcaacggc cccgaagtga
     2821 ccatgcacaa ggacgccaaa cacgccggcg actgggagca cagagacatc cacaatctgt
     2881 acggcctgta cgtgcacaag tccacctggg atggcctgat gctgcggagc aacggcgtgg
     2941 aaagaccctt tgtgctgacc cgggccttct tcgtgggcag ccagcagaca gctgctgtgt
     3001 ggaccggcga caacaccgcc gattggagcc acctgaaggt gtccacatcc atgctgctgt
     3061 ctatcagcat cgtgggcatc accctgtgtg gcgccgatgt gggcggcttc ttcggcaacc
     3121 ctgatagcga gctgctgacc agatggtatc aggccgctgc ctaccagccc ttctttaggg
     3181 cccacgccca cattgacagc aagcggagag aaccctggct ggtggcctcc gagtacatcg
     3241 accccatccg gaaggccatt caggcccgct accatctgct gccctactgg tacaccctgt
     3301 ttgccagagc cgaggccaac ggccagcctg tgatggcccc tatgtggttc cacttcccca
     3361 aggacgtgaa caccttcgac ctggacgatc agtacatggt gggagaggcc gtgctcgtgc
     3421 ggcctgtgac agatcagggc gcctctagcg tgcagctgta cttccctaag ggcacctggt
     3481 atcactaccc cagcctggaa gtgtttgccg gcggacagtg gatccagtac cccgtgacca
     3541 tcaacagcat ccccgtgttc tacagaggcg gctggatcat cccccggaaa gagagaatcc
     3601 ggcggagcag ctggctgatg agaggcgacc cttatacctt cgtggtgttc ctggaccccc
     3661 aggaacctga cgccgtgggc tacatctaca tcgacgactt ccacagcacc agcaagagca
     3721 acgcccagtt cttcaagatc atctaccaga gggtggtgga tcccaccggc gctggcgtgc
     3781 acggcggcag actgagactg cagagactgc ctctgcccgg cgagactagc atcgtgctgc
     3841 ccaaggatga tgtgctgatc cccaagatcg agcgcttcgt gatcgtgggc ttcaacagcc
     3901 ctctggaacg cgtgacagtg atcgacgccc acaagcccag acggaacctg ggctttagcg
     3961 tgacccccag ctccgtgttc agcgccggct ttaagcacac cccctccatt gtggtcgtgc
     4021 ggaagcccga cctgtccctg aacgatgagt gggaggtgca cttcgtgacc ggcaaagaga
     4081 gccgggacga cctgggcgcg ccgtcgacct ccatcacggc ctataagagt gagggggagt
     4141 cagcggagtt ctccttccca ctcaaccttg gagaggaaag cctgcaggga gagttgagat
     4201 ggaaggcaga gaaggctcct tcttcccagt cctggatcac cttctcccta aagaaccaaa
     4261 aggtgtctgt gcagaagtct actagcaacc ccaagttcca gctgtccgaa acgctcccac
     4321 tcacccttca gataccccag gtctcccttc agtttgctgg ttctggcaac ctgaccctga
     4381 ctctggacag agggatactg tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc
     4441 ccgacagcaa cactttgacc tgtgaggtga tgggacccac ctcacccaag atgagactga
     4501 tcttgaagca ggagaatcag gaggccaggg tctccaggca ggagaaagtg attcaagtgc
     4561 aggcccctga agcaggggtg tggcaatgtc tactgagtga aggtgaagag gtcaagatgg
     4621 actccaagat ccaggtttta tccaaagggt tgaattccgg cagcctgcac cacatcctgg
     4681 acgcccagaa gatgctgtgg aaccacaggg acaggaacct gcctcctctg gcccctctgg
     4741 gcccccacca ccatcaccac cattgagtct agacacgtga ttaattaagg atcccccgac
     4801 ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     4861 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag
     4921 atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     4981 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     5041 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     5101 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     5161 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     5221 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     5281 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     5341 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     5401 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     5461 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     5521 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     5581 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     5641 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     5701 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     5761 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     5821 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     5881 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     5941 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     6001 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     6061 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     6121 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     6181 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     6241 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     6301 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     6361 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     6421 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     6481 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     6541 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     6601 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     6661 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     6721 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     6781 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     6841 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     6901 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     6961 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     7021 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca
     7081 tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     7141 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     7201 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7261 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7321 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7381 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7441 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7501 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     7561 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7621 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7681 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7741 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7801 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     7861 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7921 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7981 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8041 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8101 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8161 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8221 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8281 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8341 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8401 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8461 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8521 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8581 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8641 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8701 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8761 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8821 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8881 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8941 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     9001 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     9061 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     9121 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     9181 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     9241 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     9301 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca
     9361 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
     9421 actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc atattagtca
     9481 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
     9541 ataatatgta catttatatt ggctcatgtc caatatgacc gccatgtt
//
