LOCUS       sequence-234040-       11379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 11379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_135210-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..11379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6157..6174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6226..6281
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6280..6299)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8517..8535)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8603..8707
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8708..8776,8777..9568)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8926..8945)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9739..10327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10228..10247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10481..10498
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10615..10636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10651..10656,10657..10674,10675..10681)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10689..10705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10694..10716
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10713..10729
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10713..10729
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        11000..11379
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccattcagc agcaccagca gcagcagaaa
      841 aaccagaacc agaagatcga ccacaagctg aagcggaaca acctgatgaa caagaccagc
      901 cagctgagcc ggttcaacca tcagcagcac agctaccacg tgcagcccaa gatgatgaac
      961 gtgaacctga acgagagcga cgagatgaag aacgccacca aggacgtgat caacctgatc
     1021 accggcctga agaacaacaa caatgacgac gacgatgaga caagcctgat cgacgaagag
     1081 gaagatatgg aactcatcga cgagctgaaa gagtacatgc ccaacgtgtg cttcgagaca
     1141 aaccccaagc cccggcagta ccggcagcgg atccagatcc cctacaccaa gctgaccaga
     1201 gtgtgggagc ccaactgcga gacagagggc aactggtgca tcaagtacaa agagcagacc
     1261 cactacgccg tgcactacaa gaccgtgttc aagaccgccc ccaccaagca gctgcggtgc
     1321 tgccctggct tcaccagaca gcctggctac gagggctgcg tgcccgtggc cagcgactgc
     1381 aacgagctga acaactactg ccagaacgac tgcggccaga tccccggcag ccggaactgc
     1441 agatgccccc ctggctactt cctggccccc gacaagcggc ggtgcctgga catcgacgag
     1501 tgcacctacg acagaggcgg ctgcatgtac ttttgcgaga acctgcctgg cggctacaga
     1561 tgcgcctgcc ccagcgatat ggtgctggcc cctgataaga tcagctgcat cccattcttc
     1621 aacccctgcg agcacccccc caacggcgac tgcgagcaca tctgtaccag cctgcccggc
     1681 taccggtaca tgtgcagctg caagccaggc taccacctgg ccgagaacaa gaagcggtgc
     1741 ctgcccgaca acccctgcca gatcaacaat ggcggctgcc agcacgagtg ccacaacgtg
     1801 aacggcgtgg tgcactgcag ctgccacgag ggctaccagc tggtgcctca cgacagcaag
     1861 aagtgcaccg atatcaacga gtgcagcatg aacaacggcg ggtgtcagca gctgtgccgg
     1921 aacgtggacg gcagctacag ctgcgactgc aatcccggct ggcagatcgg caacgacggc
     1981 aagagctgct accaggtgtc cgacccctgc cgcttcgaca acggcggctg cacccacatc
     2041 tgcagcagaa ccgatagcgg caccgccgag tgcagctgct cccccggata cgatctgcag
     2101 gccgacggca gaacctgcag cgaccgggat gagtgcgtga tcggcgaggc caagtgctac
     2161 ggccaggccg tgatctgcat caacgagccc ggcacataca gatgcgagtg ccagaccggc
     2221 ttccggatca gccccgatgg ccacagctgc caggatatcg atgagtgcct ggacgacaat
     2281 ggcggatgcg agttccagtg cgtgaacacc atcggcggcc accggtgcct gtgcgacttc
     2341 ggcgacgaac tgaaccccga cggccggacc tgctcccggt ctagcagcta cgtgcgggac
     2401 gacagcagca ccctgttcac ccctaccaag cagaaccgga agcagctgaa catgaagccc
     2461 acccagcgga tcgtgatcgg ggacgtgcac cagttcagca agccccggac cagcttcgac
     2521 agccggggct ctctgtgcct gcctggcaga ttcggacacg actgcagctt cacctgtagc
     2581 cactgcgaga acggcggcag atgcaaccgg gacctgaccg gctgcgagtg ctccagcggc
     2641 tacaagggca tcatctgcga ggaaaagtgc cccaagggcc tgtggggcgt ggactgcaga
     2701 cagtactgcg actgcgccaa cgacgtggac tgcgactccg tgaccggcgt gtgccagtgc
     2761 cctctgggca gacacggcac cagatgcgag ctgtctggct gccaggccgg cttttggggc
     2821 cctcagtgcg acagactgtg ccccaaccac tgcgccacca accagtgcga tagagagaag
     2881 ggccactgta cctgcagccc cggcacctac ggcagccact gccacctgag ctgccccgag
     2941 aatacctggg gcgagctgtg cagcctgaag tgccctgccg agtgtctgga cagcagacgg
     3001 cacaccaagg gctgcgaccc tcagaccggc gagtgtatct gcaagcccgg ataccagccc
     3061 cccgactgca tcctgccctg ccctcccagc tacttcggct tcaactgcaa gatgacctgc
     3121 ttcgactgcc agaatggctg tgaccctgtg accggccggt gcctggtgga atgccctcct
     3181 ggcaagcacg gcctgacctg cgagcaggac tgccctccag gctactgggg caagcagtgc
     3241 tcccggcact gtagatgcgg ctacgacgag aagggcaaca tccggacctg cgaccccacc
     3301 agcggcacct gttcttgcaa ggccggctac agaggcaaga actgcgacga gccctgcgag
     3361 atcggcacat acggccccaa ctgccggctg aagtgccagt gcggcatcag caacatcggc
     3421 tgcgatcccg tgacaggact gtgtcagtgc cccaacggca tcgtgggcca caagtgctcc
     3481 atgccctgcc cacccggcag atggggcaag tcctgcaccg agatctgcga ttgcgagaac
     3541 agcgtggaat gccacaagaa caccggcctg tgcatctgct tccccggctt catcggcaac
     3601 aagtgcgacc agaagtgcga gaaggggaag tacggcctga actgcaccga aacctgcgcc
     3661 tgcgtgcacg gcacctgtga ccacagatct ggcgcctgcg agtgcgagcc tggctggcgg
     3721 aacatgctgt gcaacaagcc ctgcgcccct ggcttctacg gcgccgattg ccagcacaga
     3781 tgcctgtgca ccaacggcgc cgagtgcgac ccagtgaccg gggactgcga gtgcaacgag
     3841 gggtggtacg gccctgcctg cgaccagcct tgtagcgacg gcacctatgg caagaattgc
     3901 gtgaagaagt gcaactgcgc cgacgtgccc ggcgtgtcct gcgacaaagt gaccggaaga
     3961 tgcatctgtc cccctggccg gaccggcaat gagtgcagcg agctgtgtcc cagcggcttc
     4021 tggggcgagg gctgctccag aacctgtgcc tgtctgagag gcgccacatg cgaccctatc
     4081 accggcaagt gccggtgcag cagcgggtgg tatggcgagg cctgcgagct gccttgtcag
     4141 agcggccact ggggcgagtc ctgtagcgtg ccctgtcagt gcagcgccaa caccagagat
     4201 cccgagggcg gcgagtgcga tcggttcaca ggcgagtgca gatgtcccca gggcttcacc
     4261 ggccccagat gccaggacaa gtgcccccca ggcagatacg gcagcgattg caagctgagc
     4321 tgtccctgcc agaaacctgg cagcagatgc gatcctgtga ctggcgcctg tgaatgtccc
     4381 cccggatggt tcggcgagac atgcgagagg ccttgcctgc tgggcttcta tggcaccaac
     4441 tgcaagcagc ggtgcagctg ccctagcggc cagccttgtc accacgagac aggcctgtgc
     4501 tcctgccccg ctggcaaata cggcccacag tgcgaggata cctgccccga gggaagatgg
     4561 ggcgaggact gtcagcacat ttgcgattgc cacctcaaaa acaacaacat gctctgcgat
     4621 cccatccacg gcgtgtgcta ctgccggcct ggatggctgg gccccaattg catcagccgg
     4681 tgcccagccg gctactttgg aatcaactgt cagcatgagt gcaagtgcaa gaacaatggc
     4741 gtgtgtcacc ccgacaacgg cacatgtatc tgccaggctg gctactatgg cgagcggtgc
     4801 cagctgaact gtcctcccgg cttttacggc ctgcagtgca agcagaagtg cggctgtatc
     4861 cacggggagg cctgcaacga gatcaccggg gagtgctact gctccgccgg ctaccacggc
     4921 aagaagtgtg aacagacctg cctgccaggg acctacggcc cccagtgtag cctgatctgc
     4981 cccgtgtgcc acacccggat cgccgactcc aacaccctgg acagcctgct gctgagcgag
     5041 acaagctcta tccccgacaa caccttcctg agcagccagt accacagcga agtgatgaag
     5101 aacaccaagg tggccatcct gcggcggctg aaccagaatc agaacaaccc tacctgtcac
     5161 ccccagaccg gacagtgccc ctgtcctcct ggcagcgagg gcaaggattg cagcaccccc
     5221 tgccccaagg accgcttcgg ccctgattgc gcccagttcg tgctggtggt catcctgggg
     5281 aagtgcgtgc acaagctggt caacatcgtg aagtccgtga tcagccggct ggacagcgtg
     5341 tacgcccact gggacatcat ggaaaagcac gccgccgtgt acgtgcacgc cgtgaacatg
     5401 ggcctggtgg tgcccaactt cgccagagtg aaaaccatgc agttcgtgca gctgatcctg
     5461 ggcaccgtga tcgtgtgcct ggacctgctg gatctgaccg tgcagggcgc gccgtcgacc
     5521 tccatcacgg cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt
     5581 ggagaggaaa gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag
     5641 tcctggatca ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac
     5701 cccaagttcc agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt
     5761 cagtttgctg gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa
     5821 gtgaacctgg tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg
     5881 atgggaccca cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg
     5941 gtctccaggc aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt
     6001 ctactgagtg aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg
     6061 ttgaattccg gcagcctgca ccacatcctg gacgcccaga agatgctgtg gaaccacagg
     6121 gacaggaacc tgcctcctct ggcccctctg ggcccccacc accatcacca ccattgagtc
     6181 tagacacgtg attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta
     6241 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     6301 gggcaaatca tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc
     6361 cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa
     6421 tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg
     6481 gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac
     6541 atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
     6601 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
     6661 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
     6721 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
     6781 ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac
     6841 taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt
     6901 agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt
     6961 tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg
     7021 aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc
     7081 gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa
     7141 aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat
     7201 tgtgctatga caccaatata accctcacaa accccttggg caataaatac tagtgtagga
     7261 atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag
     7321 actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata
     7381 tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt
     7441 tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg
     7501 ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa
     7561 gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac
     7621 gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc
     7681 gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac
     7741 ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa
     7801 ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt
     7861 cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga
     7921 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     7981 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     8041 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     8101 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg
     8161 tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc
     8221 atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc
     8281 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     8341 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     8401 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     8461 atatgctatc ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct
     8521 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     8581 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8641 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8701 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8761 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8821 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8881 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8941 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     9001 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     9061 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     9121 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     9181 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9241 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9301 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9361 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9421 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9481 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9541 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9601 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9661 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9721 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9781 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9841 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9901 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9961 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    10021 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    10081 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    10141 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    10201 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    10261 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    10321 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    10381 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    10441 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    10501 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    10561 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
    10621 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
    10681 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
    10741 caagctctag ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg
    10801 gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg
    10861 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
    10921 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
    10981 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
    11041 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
    11101 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
    11161 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
    11221 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
    11281 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
    11341 tggcagtaca tctacgtatt agtcatcgct attaccatg
//
