LOCUS       sequence-234046-        7992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_019030-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3150..3167
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3219..3274
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3273..3292)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5510..5528)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5596..5700
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5701..5769,5770..6561)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5919..5938)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6732..7320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7221..7240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7474..7491
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7608..7629
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7644..7649,7650..7667,7668..7674)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7682..7698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7687..7709
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7706..7722
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7706..7722
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccgataccc
     1201 ccgccaactg tacctatgag gacgcccacg gccggtggaa gttccacatc ggcgactacc
     1261 agagcaagtg ccccgagaag ctgaacagca agcagagcgt ggtcatcagc ctgctgtacc
     1321 ccgatatcgc catcgacgag ttcggcaacc ggggccactg gaccctgatc tacaaccagg
     1381 gcttcgaagt gaccatcaac caccggaagt ggctggtcat cttcgcctac aagagcaacg
     1441 gcgagttcaa ctgccacaag agcatgccca tgtggaccca cgacaccctg atccggcagt
     1501 ggaagtgctt cgtggccgag aagatcggcg tgcacgacaa gtttcacatc aacaagctgt
     1561 tcggcagcaa gagcttcggc cggaccctgt accacatcaa ccccagcttc gtggacaaga
     1621 tcaacgccca ccagaagtct tggagagccg agatctaccc cgagctgagc aagtacacca
     1681 tcgatgagct gcggaacaga gccggcggag tgaagtccat ggtcacccgg cccagcgtgc
     1741 tgaacagaaa gacccccagc aaagagctga tcagcctgac cggcaacctg cccctggaat
     1801 tcgactggac cagcccccct gacggcagca gatcccccgt gacccccatc cggaatcagg
     1861 gcatctgcgg cagctgctac gccttcgcct ctgccgccgc tctggaagcc cggatccggc
     1921 tggtgtccaa cttcagcgag cagcccatcc tgagccccca ggccgtggtg gactgcagcc
     1981 cttacagcga gggctgcaac ggcggcttcc ccttcctgat cgccgggaag tacggcgagg
     2041 acttcggctt tgtgtccgag aactgcgacc cctacaccgg cgaggacacc ggcaagtgca
     2101 ccgtgtccaa gaattgcacc cggtactaca ccaccgacta cagctacatc ggcggctact
     2161 acggcgccac caatgagaag ctgatgcagc tggaactgat cagcaacggc cccttcccag
     2221 tgggcttcga ggtgtacgag gacttccagt tctacaaaga gggcatctac caccacacca
     2281 ccgtgcagaa cgaccactac aacttcaacc ccttcgagct gaccaaccac gccgtgctgc
     2341 tcgtgggcta cggcgtggac aagctgagcg gcgagcccta ctggaaagtg aagaacagct
     2401 ggggcgtgga atggggcgag cagggctact tccggatcct gagaggcacc gacgagtgcg
     2461 gcgtggaaag cctgggcgtc agattcgacc ccgtgctggg cgcgccgtcg acctccatca
     2521 cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac cttggagagg
     2581 aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc cagtcctgga
     2641 tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt
     2701 tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc cttcagtttg
     2761 ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag gaagtgaacc
     2821 tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag gtgatgggac
     2881 ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc agggtctcca
     2941 ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga
     3001 gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa gggttgaatt
     3061 ccggcagcct gcaccacatc ctggacgccc agaagatgct gtggaaccac agggacagga
     3121 acctgcctcc tctggcccct ctgggccccc accaccatca ccaccattga gtctagacac
     3181 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     3241 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     3301 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     3361 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     3421 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     3481 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     3541 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     3601 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     3661 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     3721 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     3781 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     3841 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     3901 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     3961 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     4021 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     4081 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     4141 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     4201 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     4261 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     4321 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     4381 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     4441 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     4501 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     4561 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     4621 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     4681 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     4741 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     4801 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     4861 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     4921 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     4981 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     5041 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     5101 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     5161 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     5221 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     5281 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     5341 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     5401 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     5461 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     5521 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5581 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5641 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5701 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5761 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5821 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5881 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5941 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6001 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6061 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6121 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6181 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6241 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6301 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6361 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6421 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6481 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6541 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6601 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6661 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6721 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6781 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6841 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6901 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6961 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7021 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7081 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7141 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7201 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7261 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7321 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7381 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7441 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7501 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7561 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7621 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7681 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     7741 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     7801 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
     7861 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
     7921 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
     7981 gaccgccatg tt
//
