LOCUS       sequence-234050-        6906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_166340-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1684..1701
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1753..1808
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1807..1826)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4044..4062)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4130..4234
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4235..4303,4304..5095)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4453..4472)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5266..5854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5755..5774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6008..6025
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6142..6163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6178..6183,6184..6201,6202..6208)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6216..6232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6221..6243
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6240..6256
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6240..6256
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6527..6906
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccctggatt gctacagctg cctgctgtgc
      841 cccaacccct tcaaccccag cagccccctg gtgcacaact acagcaactg caagtggtgc
      901 gccaagctgg aagtgcccgg ctacagcaac cccatccggc agtgcacccc cagatgcgac
      961 ttcgagtact tcagcaagac ctaccccaag ctgcggtact actgctgcca gtacagctac
     1021 tgcaacgagt ctggcgcgcc gtcgacctcc atcacggcct ataagagtga gggggagtca
     1081 gcggagttct ccttcccact caaccttgga gaggaaagcc tgcagggaga gttgagatgg
     1141 aaggcagaga aggctccttc ttcccagtcc tggatcacct tctccctaaa gaaccaaaag
     1201 gtgtctgtgc agaagtctac tagcaacccc aagttccagc tgtccgaaac gctcccactc
     1261 acccttcaga taccccaggt ctcccttcag tttgctggtt ctggcaacct gaccctgact
     1321 ctggacagag ggatactgta tcaggaagtg aacctggtgg tgatgaaagt gactcagccc
     1381 gacagcaaca ctttgacctg tgaggtgatg ggacccacct cacccaagat gagactgatc
     1441 ttgaagcagg agaatcagga ggccagggtc tccaggcagg agaaagtgat tcaagtgcag
     1501 gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag gtgaagaggt caagatggac
     1561 tccaagatcc aggttttatc caaagggttg aattccggca gcctgcacca catcctggac
     1621 gcccagaaga tgctgtggaa ccacagggac aggaacctgc ctcctctggc ccctctgggc
     1681 ccccaccacc atcaccacca ttgagtctag acacgtgatt aattaaggat cccccgacct
     1741 cgacctctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     1801 gtctctcact cggaaggaca tatgggaggg caaatcattt ggtcgagatc cctcggagat
     1861 ctctagctag aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc
     1921 cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg
     1981 ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag
     2041 ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag
     2101 cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga
     2161 agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag
     2221 gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg
     2281 gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc
     2341 ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg
     2401 ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc
     2461 caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa
     2521 ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa
     2581 tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa
     2641 cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa
     2701 tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc
     2761 ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa
     2821 tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta
     2881 tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca
     2941 gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg
     3001 gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc
     3061 tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg
     3121 agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg
     3181 tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa
     3241 aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg
     3301 cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag
     3361 ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg
     3421 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     3481 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     3541 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     3601 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     3661 catatgctat cctaatagag attagggtag tatatgctat cctaatttat atctgggtag
     3721 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     3781 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     3841 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     3901 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     3961 tgctatccta atctgtatcc gggtagcata tgctatcctc atgataagct gtcaaacatg
     4021 agaattttct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4081 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4141 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4201 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4261 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4321 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4381 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4441 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     4501 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4561 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4621 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4681 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4741 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     4801 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4861 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4921 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4981 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5041 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5101 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5161 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5221 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5281 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5341 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5401 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5461 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5521 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5581 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5641 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5701 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5761 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5821 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5881 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5941 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6001 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     6061 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     6121 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     6181 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     6241 aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacc aattctcatg
     6301 tttgacagct tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac
     6361 tggtaggtat ggaagatcta tacattgaat caatattggc aattagccat attagtcatt
     6421 ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat
     6481 aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttgaca ttgattattg
     6541 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     6601 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     6661 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     6721 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     6781 ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     6841 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     6901 accatg
//
