LOCUS       sequence-234054-       10347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  construction intermediate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan 
            YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, 
            Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
  TITLE     Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
  JOURNAL   Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z.
  PUBMED    30617347
REFERENCE   2  (bases 1 to 10347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z."
COMMENT     Sequence Label: pJMP1290
FEATURES             Location/Qualifiers
     source          1..10347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             10..99
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     oriT            1269..1377
                     /note="incP origin of transfer"
     rep_origin      1646..2034
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(join(2335..3126,3127..3195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2958..2977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3196..3300)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      4220..4314
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      4417..4503
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    4554..4601
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(4669..4905)
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     promoter        complement(join(5231..5236,5237..5253,5254..5259))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    5350..5397
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(join(5793..5799,5800..5816,5817..5822))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             join(6244..6270,6271..6273,6274..8760,8761..8763,
                     8764..10347)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gcggccgcgg agcagaaact catctctgaa gaagatctgg aacaaaagtt gatttcagaa
       61 gaagatctgg aacagaagct catctctgag gaagatctgt aataaggcgc gcctccttaa
      121 tgggacttgc agcctcggta ccaaattcca gaaaagacac ccgaaagggt gttttttcgt
      181 tttggtccca cagaatgagc atcatggctc tagtcgaccg ggtaggggca tagcttagat
      241 aattggaaaa gaggaaaaaa gcttaatctt ttttcgaagg ttaagctttt tcttttattt
      301 ataaaaagtg aactaactat cagaaagaaa ttatattaaa ttttattttt ttgtttaaaa
      361 agtagattat ataaaggcaa gctaggtggg ggaaaatatg tttaaaaaag aaaaagtcac
      421 agaatacatt tggactatac taataccaac aatcatcact tttatcatta gttgggttgg
      481 gtcttattac aatggtactt cgacagttag tattggacaa cctacaaaag tttccggtca
      541 gtatatcacg ccaataaata taagtcccta tcatgatatt aaggaattaa gaataacttt
      601 tccgcaaaaa ctagatgtaa aacaaattag ttcaaatgag cctataaatg taaaatcaga
      661 taagaacaat ataggagttg aaagtaattc cacttttgag attgcgaaaa tcgttgaaaa
      721 taatagcgtt cagttgctaa ttacaacaca aaaaaagtta aacgataagg aaattagaat
      781 tgataaaaat ggaaataaca tttctgtaaa ttatgaatct cagattgtta atcctgcaaa
      841 aaaacaatta atcaatctta taattacgtc atctatttat tttataatgc ttaatatact
      901 agcattgatt atgaacaaaa gatgggataa gtattatgca aaaatgaaaa atgaaatcaa
      961 agaatttgag gataatgcaa aagatcttga taaaaaatca aagaagaaaa gcgaggaatt
     1021 atcggagctg cgaaagacct tgaaccaagc gtttgaggaa actgatagga taaaatatca
     1081 tgagaagaaa aaacaaatcc tcctcttagc taagttaaac gattataaaa aagaactaac
     1141 cttttggaga aatacaataa gaaaagttct ttatgaactt cctgatggag ataaaaaagc
     1201 agataaacta atagggacag ttacatcatc tttaaaaacg tacggtacag tcgaaaagta
     1261 aagcttatcg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
     1321 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
     1381 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
     1441 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
     1501 gcagcggaaa gtatacctta aggaatcccc tgataacgca ggaaagaaca tgtgagcaaa
     1561 aggccagcaa aaggccagga accgtaaaaa ggccgcactc cctgcctctg tcatcacgat
     1621 actgtgatgc catggctaat tcccatgtca gccgttaagt gttcctgtgt cactcaaaat
     1681 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac
     1741 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
     1801 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
     1861 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
     1921 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga
     1981 aacatgagag cttagtacgt actatcaaca ggttgaactg ctgatcttca gatcctctac
     2041 gccggacgca tcgtggccgg atcttgcggc cgcaaaaatt aaaaatgaag ttttaaatca
     2101 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2161 ccaataactg cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2221 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2281 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     2341 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     2401 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     2461 tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     2521 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     2581 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     2641 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     2701 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     2761 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     2821 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     2881 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     2941 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3001 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     3061 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     3121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     3181 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     3241 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     3301 cacatttccc cgaaaagtgc cacctgacgt cttctaacta cttattttaa agcagtctgg
     3361 attgtttggg taattcataa aaaaataaaa gaaagaagga ggaatagagt ttttcttttt
     3421 tttgtttgca atgttactgt caagtcgcaa aagaattgct atgaccgaaa aaaaaccgca
     3481 aaggaataga tataaggtac ctttttgcaa ttcatctttg taaaataaag gttattctga
     3541 cataatacaa ttaatgtaaa aattcgcaca attttatgta aggatggggg aattttcttg
     3601 cggggtgttt tcttagataa agataaaatt ccgtacgact tagtcacgaa aaagttaaat
     3661 gaatggtata catcaataaa aaatgatcaa gttgagcaag ccgagattat aaaaacagaa
     3721 gtagagaaag aattgttaaa catggaagaa aatcaagatg ccctgttata ttatcaacta
     3781 ttagaattta gacatgagat aatgctgagt tatatgaaat ctaaggaaat agaagatctc
     3841 aataatgctt atgagactat aaaagaaatt gagaagcaag ggcaattaac tggcatgttg
     3901 gaatactatt tttacttttt taagggtatg tacgagttta ggcgtaaaga attaatttca
     3961 gcgataagtg cttatcgaat agctgaatca aagttgtcag aagttgagga tgaaatagag
     4021 aaagcagagt tttttttcaa agtgtcctat gtatattatt atatgaaaca aacatacttc
     4081 tccatgaatt atgcaaatcg tgcactcaaa atatttagag agtatgaaga atatgctgtc
     4141 cagactgtgc gttgtcaatt tattgtagca ggaaacttga tcgagtgaat cgataagcta
     4201 gcttaattag ctgagcttgg actcctgttg atagatccag taatgacctc agaactccat
     4261 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc
     4321 tagactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt
     4381 aaacgcctgg ggtaatgact ctctagcttg aggcatcaaa taaaacgaaa ggctcagtcg
     4441 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     4501 aatccgccgc taggagcttg cggcccggac gatgagctcg aattggggat cttgaagtac
     4561 ctattccgaa gttcctattc tctagaaagt ataggaactt cagagcgctt ttgaagctga
     4621 tgctcgagtg cttaaaaact tactcaatgg aataattcta gataattctt aggccacacg
     4681 ttcaagtgca gccacaggat aaatttgcac tgagcctggg tgggattcgg actcgaccgc
     4741 atagccttca ggagtgagtt ttgtgcaata ccaaccgacg acttgaccct gccaagcggc
     4801 accagatttc ttgcgtacgc gatcccctaa gccaaaggtg gcactcaggg gaagcgcaaa
     4861 ctgccctgca acgggagcgt tggcttcatc gctactttga cccatgtcga atccttcttg
     4921 tgaatctatt atggcgacaa gcaaatcgag ctctgactgc ctaccccaca acaactatca
     4981 gaaagcacca gcacaacggc tgcctaactt tgttttaggg cgactgccct gctgcgtaac
     5041 atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga
     5101 tgcccgaggc atagactgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc
     5161 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta
     5221 cttgcattac agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg aattatccat
     5281 tgctgttgac aaagggaatc aggctcgaga gttcaacaga cagccttcgt tcttatggcc
     5341 gttcaggagg aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcttg
     5401 agaagagaaa agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta gtggacaaga
     5461 ccggcggtct taagtttttt ggctgaaacg aatgacgaaa aggctgtctg agcaaatcca
     5521 ggaggtcgtt taaaccgtat tagcccggta ttttggaaat agcggaagca ctgacttttg
     5581 ttatcaataa aaaaggcccc ccgttaggga ggccttattg ttcgtctact cggaagagcg
     5641 agagacaaca gaacggtcag ccacatgaat tcaaaaaaaa agcaccgact cggtgccact
     5701 ttttcaagtt gataacggac tagccttatt taaacttgct atgctgtttc cagcatagct
     5761 cttaaacaga ccgctaaact gaaagttcca cacattatac gagccggatg attaattgtc
     5821 aacagctcat ttcagaatat ttgccagaac cggaattctt tcagctcagt cgataggtag
     5881 taggcaagag tagtcgcacc tttggtcgaa aaaaaaagcc cgcactgtca ggtgcgggct
     5941 tttttctgtg tttccccaaa agtaaaaacc cgccgaagcg ggtttttacg taaaacaggt
     6001 gaaactgacc agacgagaag gctttggagg tgataatggg gctcaaggac ccgggaggca
     6061 gataggtagg catggccccc attttcaata caagcaacgc atgagaaagc ccccggaaga
     6121 tcaccttccg ggggcttttt tattgcgctc cggcaattaa aaaagcggct aaccacgccg
     6181 ctttttttac gtctgcagag ctcataggca agcgaatcgt gatgcctctt agccagtact
     6241 agtatggata agaaatactc aataggctta gctatcggca caaatagcgt cggatgggcg
     6301 gtgatcactg atgaatataa ggttccgtct aaaaagttca aggttctggg aaatacagac
     6361 cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg
     6421 gaagcgactc gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt
     6481 tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat
     6541 cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt
     6601 ggaaatatag tagatgaagt tgcttatcat gagaaatatc caactatcta tcatctgcga
     6661 aaaaaattgg tagattctac tgataaagcg gatttgcgct taatctattt ggccttagcg
     6721 catatgatta agtttcgtgg tcattttttg attgagggag atttaaatcc tgataatagt
     6781 gatgtggaca aactatttat ccagttggta caaacctaca atcaattatt tgaagaaaac
     6841 cctattaacg caagtggagt agatgctaaa gcgattcttt ctgcacgatt gagtaaatca
     6901 agacgattag aaaatctcat tgctcagctc cccggtgaga agaaaaatgg cttatttggg
     6961 aatctcattg ctttgtcatt gggtttgacc cctaatttta aatcaaattt tgatttggca
     7021 gaagatgcta aattacagct ttcaaaagat acttacgatg atgatttaga taatttattg
     7081 gcgcaaattg gagatcaata tgctgatttg tttttggcag ctaagaattt atcagatgct
     7141 attttacttt cagatatcct aagagtaaat actgaaataa ctaaggctcc cctatcagct
     7201 tcaatgatta aacgctacga tgaacatcat caagacttga ctcttttaaa agctttagtt
     7261 cgacaacaac ttccagaaaa gtataaagaa atcttttttg atcaatcaaa aaacggatat
     7321 gcaggttata ttgatggggg agctagccaa gaagaatttt ataaatttat caaaccaatt
     7381 ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac taaatcgtga agatttgctg
     7441 cgcaagcaac ggacctttga caacggctct attccccatc aaattcactt gggtgagctg
     7501 catgctattt tgagaagaca agaagacttt tatccatttt taaaagacaa tcgtgagaag
     7561 attgaaaaaa tcttgacttt tcgaatccct tattatgttg gtccattggc gcgtggcaat
     7621 agtcgttttg catggatgac tcggaagtct gaagaaacaa ttaccccatg gaattttgaa
     7681 gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg aacgcatgac aaactttgat
     7741 aaaaatcttc caaatgaaaa agtactacca aaacatagtt tgctttatga gtattttacg
     7801 gtttataacg aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa accagcattt
     7861 ctttcaggtg aacagaagaa agccattgtt gatttactct tcaaaacaaa tcgaaaagta
     7921 accgttaagc aattaaaaga agattatttc aaaaaaatag aatgttttga tagtgttgaa
     7981 atttcaggag ttgaagatag atttaatgct tcattaggta cctaccatga tttgctaaaa
     8041 attattaaag ataaagattt tttggataat gaagaaaatg aagatatctt agaggatatt
     8101 gttttaacat tgaccttatt tgaagatagg gagatgattg aggaaagact taaaacatat
     8161 gctcacctct ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg
     8221 ggacgtttgt ctcgaaaatt gattaatggt attagggata agcaatctgg caaaacaata
     8281 ttagattttt tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct gatccatgat
     8341 gatagtttga catttaaaga agacattcaa aaagcacaag tgtctggaca aggcgatagt
     8401 ttacatgaac atattgcaaa tttagctggt agccctgcta ttaaaaaagg tattttacag
     8461 actgtaaaag ttgttgatga attggtcaaa gtaatggggc ggcataagcc agaaaatatc
     8521 gttattgaaa tggcacgtga aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag
     8581 cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa gtcagattct taaagagcat
     8641 cctgttgaaa atactcaatt gcaaaatgaa aagctctatc tctattatct ccaaaatgga
     8701 agagacatgt atgtggacca agaattagat attaatcgtt taagtgatta tgatgtcgat
     8761 gccattgttc cacaaagttt ccttaaagac gattcaatag acaataaggt cttaacgcgt
     8821 tctgataaaa atcgtggtaa atcggataac gttccaagtg aagaagtagt caaaaagatg
     8881 aaaaactatt ggagacaact tctaaacgcc aagttaatca ctcaacgtaa gtttgataat
     8941 ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata aagctggttt tatcaaacgc
     9001 caattggttg aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg
     9061 aatactaaat acgatgaaaa tgataaactt attcgagagg ttaaagtgat taccttaaaa
     9121 tctaaattag tttctgactt ccgaaaagat ttccaattct ataaagtacg tgagattaac
     9181 aattaccatc atgcccatga tgcgtatcta aatgccgtcg ttggaactgc tttgattaag
     9241 aaatatccaa aacttgaatc ggagtttgtc tatggtgatt ataaagttta tgatgttcgt
     9301 aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac
     9361 tctaatatca tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa
     9421 cgccctctaa tcgaaactaa tggggaaact ggagaaattg tctgggataa agggcgagat
     9481 tttgccacag tgcgcaaagt attgtccatg ccccaagtca atattgtcaa gaaaacagaa
     9541 gtacagacag gcggattctc caaggagtca attttaccaa aaagaaattc ggacaagctt
     9601 attgctcgta aaaaagactg ggatccaaaa aaatatggtg gttttgatag tccaacggta
     9661 gcttattcag tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc
     9721 gttaaagagt tactagggat cacaattatg gaaagaagtt cctttgaaaa aaatccgatt
     9781 gactttttag aagctaaagg atataaggaa gttaaaaaag acttaatcat taaactacct
     9841 aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa
     9901 ttacaaaaag gaaatgagct ggctctgcca agcaaatatg tgaatttttt atatttagct
     9961 agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg
    10021 gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt
    10081 gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac
    10141 aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac gaatcttgga
    10201 gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata tacgtctaca
    10261 aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc
    10321 attgatttga gtcagctagg aggtgac
//
