LOCUS       sequence-234056-        7227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_054070-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2385..2402
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2454..2509
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2508..2527)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4745..4763)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4831..4935
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4936..5004,5005..5796)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5154..5173)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5967..6555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6456..6475
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6709..6726
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6843..6864
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6879..6884,6885..6902,6903..6909)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6917..6933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6922..6944
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6941..6957
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6941..6957
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccgatgtgg
     1201 tgcagaacgt ggacgtgaac ggcagccacc ccagcgtgtc cctgctgagc gccagcaacc
     1261 tgagcagccc caagaacgag cccagcgcca accagaccac ccaggccaag tggcagtacc
     1321 tgtgccccta catgaagtac aagtgcagcg agctgcccgg ctactgcctg atctgcaact
     1381 tcaacaccag ctgcgtgtac ggcaccgtgg tcaacgtgac ctgcttcccc agacccggcg
     1441 tgtactgcaa ggtgcccagc agcatcaccc aggaaaatca gaccagccag accaacagca
     1501 gcgagttcag ctaccccatg agcgaccaga ccaactacat catccagaaa cccatcgtgt
     1561 gccggttctg ccaccagctg gacgacaccg agatcgtgtg catcggccgg accaactgcc
     1621 ggcagcccaa cgtgcccaga agcttctacg agacaaagtg cgagcccttc ccccacatcc
     1681 tgtgcctggg cagacgggtg ttccggcgga tggtgccctg cgactggtcc tctggcgcgc
     1741 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     1801 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     1861 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     1921 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     1981 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     2041 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     2101 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     2161 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     2221 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     2281 ccaaagggtt gaattccggc agcctgcacc acatcctgga cgcccagaag atgctgtgga
     2341 accacaggga caggaacctg cctcctctgg cccctctggg cccccaccac catcaccacc
     2401 attgagtcta gacacgtgat taattaagga tcccccgacc tcgacctctg gctaataaag
     2461 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     2521 atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat
     2581 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     2641 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     2701 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     2761 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     2821 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     2881 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     2941 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     3001 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     3061 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     3121 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     3181 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     3241 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     3301 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     3361 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     3421 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     3481 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     3541 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     3601 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     3661 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     3721 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     3781 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     3841 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     3901 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     3961 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     4021 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     4081 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     4141 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     4201 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     4261 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4321 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     4381 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     4441 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4501 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4561 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     4621 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     4681 cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga
     4741 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4801 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4861 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4921 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4981 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5041 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5101 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5161 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     5221 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5281 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5341 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5401 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5461 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5521 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5581 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5641 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5701 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5761 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5821 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5881 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5941 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6001 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6061 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6121 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6181 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6241 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6301 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6361 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6421 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6481 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6541 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6601 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6661 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6721 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6781 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     6841 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     6901 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     6961 gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc
     7021 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     7081 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     7141 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     7201 gctcatgtcc aatatgaccg ccatgtt
//
