LOCUS       sequence-234061-        3552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part. Promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie 
            MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick
            AJ
  TITLE     CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria 
            Based on the Plant MoClo Syntax.
  JOURNAL   Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub
            2019 Feb 28.
  PUBMED    30819783
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.18.01401"; journalName: "Plant Physiol"; date: 
            "2019-05"; volume: "180"; issue: "1"; pages: "39-55"
COMMENT     Sequence Label: pC0.057
FEATURES             Location/Qualifiers
     source          1..3552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(78..172)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(328..357)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      615..646
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(745..1560)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1468..1487
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1552..1571
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(1918..2868)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2916..3138
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 ttcagactgg ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta
       61 ctgtccctag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
      121 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
      181 ctcgtaaact tggtctgaca gctctagctc cggcaaaaaa acgggcaagg tgtcaccacc
      241 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga cgtctaagaa
      301 aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag gtgagccggt
      361 ctcaggagtg ttttatcaga aaaataggtt gaatgcccct ttacctaagg cagcggagga
      421 tgtgaatgga acaatggggg caatgagatt tccataaagt actctatttt gctccttttc
      481 catcgaaaga cacggcaaca gtagaccggg gacagaccga gttgacatat caagtttttt
      541 tgatgtaaga ttattccatc aagttttttt gatgagttgt cctagagtag tggaggttac
      601 tagaatgtga gaccagtcaa aagcctccga ccggaggctt ttgactaaaa cttcccttgg
      661 ggttatcatt ggggctcact caaaggcggt aatcagataa aaaaaatcct tagctttcgc
      721 taaggatgat ttctgctagt attattagaa aaactcatcg agcatcaaat gaaactgcaa
      781 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
      841 agaaaactca ccgaggcagt tccaaagaat ggcaaggtcc tggtaacggt ctgcgattcc
      901 gacccgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
      961 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaagagct tgtgcatttc
     1021 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     1081 caaaccgtta ttcatgcgtg attgcgcctg agcaagacga aatacacgat cgctgttaaa
     1141 aggacaatta caaacaggaa tcgaatgtaa ccggcgcagg aacacggcca gcgcatcaac
     1201 aatattttca cctgaatcag gatattcttc taatacctgg aaggctgttt tcccaggaat
     1261 cgcggtggtg agtaaccacg catcatcagg agtacggata aaatgcttga tggtcgggag
     1321 aggcataaac tccgtcagcc agttgagacg gaccatctca tctgtaacat cattggcaac
     1381 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaagcgata
     1441 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     1501 gtccatgttg gagtttaagc gcggacggga gcaagacgtt tcccgttgaa tatggctcat
     1561 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     1621 tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa
     1681 tcgaactttt gctgagttga aggatcagct ctagtagtta cattgtcgat ctgttcatgg
     1741 tgaacagctt tgaatgcacc aaaaactcgt aaaagctctg atgtatctat cttttttaca
     1801 ccgttttcat ctgtgcatat ggacagtttt ccctttgata tgtaacggtg aacagttgtt
     1861 ctacttttgt ttgttagtct tgatgcttca ctgatagata caagagccat aagaacctca
     1921 gatccttccg tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca
     1981 tgagaacgaa ccattgagat catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa
     2041 actggtgagc tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttatg
     2101 taggtaggaa tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt
     2161 gttctcaagt tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt
     2221 atcagtcggg cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc
     2281 tttacttatt ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc
     2341 aagcattaac atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt
     2401 cttttgtgtt agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa
     2461 agacttaaca tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa
     2521 tatctcttca ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca
     2581 ctggaaaatc tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag
     2641 ctctctggtt gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg
     2701 agcgtattgg ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt
     2761 ggggttgagt agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata
     2821 gcgactaatc gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg
     2881 gtctaggtga ttttaatcac tataccaatt gagatgggct agtcaatgat aattacatgt
     2941 ccttttcctt tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt
     3001 aaattctgct agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat
     3061 tcaagtggtt ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc
     3121 cagccctgtg tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca
     3181 aacgctgttt gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct
     3241 cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc
     3301 gctgtctttt tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta
     3361 aatggcacta caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa
     3421 agcccgtcac gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac
     3481 tttttgctgt tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc
     3541 cgtgacaggt ca
//
