LOCUS       sequence-234064-        9642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_134800-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..9642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4420..4437
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4489..4544
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4543..4562)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6780..6798)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6866..6970
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6971..7039,7040..7831)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7189..7208)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8002..8590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8491..8510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8744..8761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8878..8899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8914..8919,8920..8937,8938..8944)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8952..8968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8957..8979
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8976..8992
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8976..8992
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        9263..9642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccaagacca gccccaacgt gtccttctgc
      841 gagctgaagc agaacgagca ccaccaccag tacgacatcc tgatcaacaa caccatcatc
      901 gagctgagct gcagcttcgg caacgagtgg tacatcccca gcaccatccc cctgaacttc
      961 aagcccatcg tgatcatgga ccagattaac aacaaccaga gcaagctgct gatccagctg
     1021 cccaagaaca acaccgacaa cctgcggacc cacttccggg gcctgtacca gtgctgccag
     1081 agcagcaaca gctacaacat cagcatcatc aactggctgc ccaacgagcc cagctgctgc
     1141 ccctgctgtc cttggtacga gcaggaccag tgcctgttca agcagatcca gttcatcaac
     1201 cacagcaaca tcatcttcaa gctgaagtcc atgaagacct actgcggcag cgtgttcctg
     1261 cacatgaaca gcgtgaccat gaccaccatc cacctggaca tcatccggaa ccagcccatg
     1321 ctgatccctt gccctggcct gaacagcacc taccaggaag atagcagcct gttcagcatc
     1381 gactaccagc tgacccccca caagagcagc taccagctgt ggtatccctt caagcagttc
     1441 aagcacagca tccagtacta ctacgacaac agattcggcc tgtgcctgct gaagtcccag
     1501 ttcccccgga tcatgctgta catgctgtgc aactacaagc agatgaacga gcagcggatc
     1561 atccagccca tctggcccct gcagagcctg gtcatcagcc ccatcatcca catctacctg
     1621 cagagcatca aagagatcga gtacaccacc tacaagctca tgaatatcag cagccacagc
     1681 ttcctcaaca acatcaattg gctgatcaag tacagcaacg aggtgctgat cttccggatc
     1741 aagcagcaca ccaagctgct gcggctgatc tgcatcggca gctacaccca gaagtacatc
     1801 gtccacaagc ccatcagcaa gctgtgcttc cggtggaaga tcctgaacca ggacaacgtg
     1861 aagctgagca acatcagcga cttccaggaa cactaccaga tcgacgagca gagcccctgg
     1921 gagagaaccg agtgcctgac cacccaccag accttcaaca agttcagcat cctgtacgcc
     1981 tactctaagt acgacctgga cctgagcaag accagcaacg agacaaacag cagcgtgaac
     2041 aataccttca tcctgtgcga agtgaccacc cccgaccgga tggcccccat cagccagaga
     2101 atcatccaga tcgtgaaaac caccaaccag atcagcaaca agctgcacat ctcctaccac
     2161 tgcaactgca tggtgctggg cgacaacaag tccagcttca gcgacaacag caccctgagc
     2221 ctgctgatca agaacaactc cgtgaacctg aacatcagct tttggaccga gcacgagaca
     2281 gacaaccagc ccttctgcat cctgatgaag cacggcatgc ccaagagcga gcagaaaaca
     2341 aaagagacac agatcaaccc catccggacc aaggacacct ggaagtgctt catcaagatc
     2401 aagatgaacc tgcccaacca gaccatgacc ctgagcatcg gctacggcga gatcaagcgg
     2461 accttccaga tcttcatcgt gaagtccatc ctggaactga cccggaacgt ggaaaacctg
     2521 cagtcccaga tcacccagca gagctacctg cactgcatcc ccaacgacat ggaacggtct
     2581 atcgcccaga gattcggcta ccactacccc aaccacgtga cctggttcat cagcatggac
     2641 aaccacatcc agaactacac cacatacaac aagaccttcg agcacatctt taagctgatc
     2701 ccattctaca acgtgaccaa ctaccccatg atcgtgctga accagaccgg caacctgtgg
     2761 gagcgcccca acgagctggg ccacctgaac aacctgtacc tgaacgactg gcccaagcag
     2821 ctgtgcgtgc ggtgcagcag cgtgtacgag atcggcatcc acttcaacag ccacgagagc
     2881 tgcaccctgc tgaacgactt caacgtgatc gacatcttca acaataccgg caaccaccgg
     2941 gatctgcagt ccatccgggg caagaaacag ctgtacttcg tgatcgagct gaacaacagc
     3001 agccagttct acgaagtgaa cagccactac aacaacagcg agatcagcct ggtgcagagc
     3061 accttcatca gcctggaatg catcagaaag ctgtacacca agcccgagca gtttaagcgg
     3121 aaagagtggc ccgagctgac cattaacaat acccagaaca ataccgagga ccacctcatc
     3181 cacaacacca gctggatcct gagcccctac agcttcaaag tgaagaaagt gatccacctc
     3241 aacaagcccc tggtcatcca cctgaagtgc gcctacaaga ccgagcagta cgtgatcaag
     3301 atccacacct ttaagcagaa caagccccag atcctgaagc ccatcgagaa catccagttt
     3361 atcaagacca acctgcagca gagcaccctc ttcaactaca ccatccacac caccaacatc
     3421 accaagaaca gcattgagct gatctgccag gccatcggcc tgcccaagcc caccatccag
     3481 tggatgaagg ccatctacaa ccacaacaag agctttatca agtggttcaa cgtgcacacc
     3541 tgtagcatcg acgacaacaa ggtgcccttc aacatctcca tcaaggacga ccagcagatt
     3601 cagacctgca tctaccacct gaccacactg aaagagacac tgaaccagag ccactaccag
     3661 tgtctggcca ccaatatcgc cggccacacc ttcaaacaga tcaaactgat ctacctggac
     3721 accaccctga tgagcagcaa cgaaaccatc cagctgcaga tcatgatggg cgcgccgtcg
     3781 acctccatca cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac
     3841 cttggagagg aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc
     3901 cagtcctgga tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc
     3961 aaccccaagt tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc
     4021 cttcagtttg ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag
     4081 gaagtgaacc tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag
     4141 gtgatgggac ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc
     4201 agggtctcca ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa
     4261 tgtctactga gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa
     4321 gggttgaatt ccggcagcct gcaccacatc ctggacgccc agaagatgct gtggaaccac
     4381 agggacagga acctgcctcc tctggcccct ctgggccccc accaccatca ccaccattga
     4441 gtctagacac gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat
     4501 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     4561 ggagggcaaa tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg
     4621 ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg
     4681 taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg
     4741 ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg
     4801 cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc
     4861 aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga
     4921 catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg
     4981 gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg
     5041 cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca
     5101 gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg
     5161 gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt
     5221 atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg
     5281 ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc
     5341 ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc
     5401 gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg
     5461 cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta
     5521 ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta
     5581 aagactggat gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca
     5641 atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt
     5701 tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac
     5761 tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac
     5821 aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca
     5881 cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac
     5941 cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa
     6001 tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac
     6061 aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga
     6121 ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct
     6181 ggatagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     6241 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     6301 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     6361 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta
     6421 gggtagtata tgctatccta atttatatct gggtagcata tactacccaa atatctggat
     6481 agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     6541 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     6601 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     6661 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     6721 agcatatgct atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg
     6781 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     6841 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6901 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6961 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7021 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7081 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7141 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7201 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7261 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7321 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7381 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7441 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7501 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7561 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7621 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7681 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7741 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7801 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7861 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7921 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7981 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8041 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8101 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8161 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8221 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8281 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8341 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8401 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8461 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8521 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8581 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8641 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8701 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8761 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8821 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     8881 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     8941 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     9001 cgccaagctc tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
     9061 cgggcaacgt tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca
     9121 ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt
     9181 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
     9241 tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
     9301 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     9361 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     9421 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     9481 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
     9541 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
     9601 acttggcagt acatctacgt attagtcatc gctattacca tg
//
